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Mitochondrial and nuclear myxothiazol resistance in Saccharomyces cerevisiae

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Summary

Mitochondrial and nuclear mutants resistant to myxothiazol were isolated and characterized. The mitochondrial mutants could be assigned to two loci, myx1 and myx2, by allelism tests. The two loci map in the box region, the split gene coding for apocytochrome b. Locus myx1 maps in the first exon (box4/5) whereas myx2 maps in the last exon (box6). The nuclear mutants could be divided into three groups: two groups of recessive mutations and one of dominant mutations. Respiration of isolated mitochondria from mitochondrial mutants is resistant to myxothiazol. These studies support the conclusion that myxothiazol is an inhibitor of the respiratory chain of yeast mitochondria. The site of action of myxothiazol is mitochondrial cytochrome b.

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Abbreviations

box :

mosaic gene coding for apocytochrome b

cyt b:

cytochrome b

MIC:

minimum inhibitory concentration

MNNG:

N-methyl-N'-nitro-N-nitrosoguanidine

Myx R/Myx S :

allelte forms of a locus conferring myxothiazol resistance

myx1, myx2 :

mitochondrial loci conferring myxothiazol resistance

rho +/rho − :

grande/cytoplasmic petite

rho 0 :

cytoplasmic petite that is deleted of all mitochondrial DNA

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Authors and Affiliations

  1. Abteilung Mikrobiologie, Gesellschaft für Biotechnologische Forschung, Mascheroder Weg 1, D-3300, Braunschweig, Federal Republic of Germany

    Georg Thierbach

  2. Fakultät für Biologie, Universität Bielefeld, Postfach 8640, D-4800, Bielefeld 1, Federal Republic of Germany

    Georg Michaelis

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  1. Georg Thierbach
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  2. Georg Michaelis
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Communicated by W. Gajewski

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Thierbach, G., Michaelis, G. Mitochondrial and nuclear myxothiazol resistance in Saccharomyces cerevisiae . Mol Gen Genet 186, 501–506 (1982). https://doi.org/10.1007/BF00337956

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