Summary
A dnaW mutant, isolated on the basis of inability to effect conjugal DNA transfer at high temperature, has been shown by complementation and enzyme assay to be defective in the adk (adenylate kinase; EC 2.7.4.3) locus. The adk mutant, known to have reduced ATP concentration at the nonpermissive temperature (Cousin and Belaich 1966), was used to demonstrate a donor energy requirement for stable aggregate formation and for chromosome transfer in conjugation.
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Communicated by G. O'Donovan
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Henson, J.M., Blinkowa, A. & Walker, J.R. The Escherichia coli dnaW mutation is an allele of the adk gene. Mol Gen Genet 186, 488–492 (1982). https://doi.org/10.1007/BF00337953
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DOI: https://doi.org/10.1007/BF00337953