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cdc7-1 A temperature sensitive cell-cycle mutant which interferes with induced mutagenesis in Saccharomyces cerevisiae

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Summary

The mutant cdc7-1 is shown here to block UV induced reversion of six different auxotrophic mutations and forward mutations at several genes concerned with adenine biosynthesis in Saccharomyces cerevisiae. Chemical mutagenesis is also drastically reduced. In its effect on mutagenesis cdc7-1 resembles rad6-1. However, in contrast to rad6-1, cdc7-1 does not affect sporulation or mitotic recombination neither is it sensitive to the antifolate drug trimethoprim. It appears to fall in the same epistatic group as rad6-1. Possible explanations for its action are briefly considered.

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References

  • Game JC, Cox BS (1973) Synergistic interactions between RAD mutations in yeast. Mutat Res 20:35–44

    Google Scholar 

  • Hartwell LH (1971) Genetic control of the cell division cycle in yeast. II. Genes controlling DNA replication and its initiation. J Mol Biol 59:183–194

    Google Scholar 

  • Hartwell LH (1973) Three additional genes required for deoxyribonucleic acid synthesis in Saccharomyces cerevisiae. J Bacteriol 115:966–974

    Google Scholar 

  • James AP, Kilbey BJ, Prefontaine GJ (1978) The timing of UV mutagenesis in yeast: continuing mutation in an excision defective rad1-1 strain. Mol Gen Genet 165:207–212

    Google Scholar 

  • Kilbey BJ, Brychcy T, Nasim A (1978) The initiation of UV mutagenesis in Saccharomyces cerevisiae. Nature (London) 274:889–891

    Google Scholar 

  • Lawrence CW (1982) Mutagenesis in Saccharomyces cerevisiae. Adv Genet (in press)

  • Lawrence CW, Christensen R (1976) UV mutagenesis in radiation-sensitive strains of yeast. Genetics 82:207–232

    Google Scholar 

  • Lawrence CW, Christensen RB (1979) Metabolic suppressors of trimethoprim and ultraviolet light sensitivities in Saccharomyces cerevisiae rad 6 mutants. J Bacteriol 139:866–676

    Google Scholar 

  • Lawrence CW, Stewart JW, Sherman F, Thomas FLX (1970) Mutagenesis in ultraviolet-sensitive mutants of yeast. Genetics 64:536–537

    Google Scholar 

  • Prakash L, Hinkle D, Prakash S (1979) Decreased UV mutagenesis in cdc8, a DNA replication mutant of Saccharomyces cerevisiae. Mol Gen Genet 172:249–258

    Google Scholar 

  • Witkin EM (1976) Ultraviolet mutagenesis and inducible DNA repair in Escherichia coli. Bacteriol Rev 40:869–907

    Google Scholar 

  • Zimmerman FK, Kern R, Rasenberger H (1975) A yeast strain for simultaneous detection of induced mitotic gene conversion and reverse mutation. Mutat Res 28:381–388

    Google Scholar 

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Authors and Affiliations

  1. Department of Genetics, University of Edinburgh, West Mains Road, EH9 3 JN, Edinburgh, Scotland

    G. D. E. Njagi & B. J. Kilbey

Authors
  1. G. D. E. Njagi
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  2. B. J. Kilbey
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Communicated by Ch. Auerbach

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Njagi, G.D.E., Kilbey, B.J. cdc7-1 A temperature sensitive cell-cycle mutant which interferes with induced mutagenesis in Saccharomyces cerevisiae . Mol Gen Genet 186, 478–481 (1982). https://doi.org/10.1007/BF00337951

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  • DOI: https://doi.org/10.1007/BF00337951

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