Summary
A sensitive radioimmuno assay (RIA) method for detection of the UV photoproduct, thymine dimers \(\left( {\overline {TT} } \right)\) has been developed. The limit of detection of this method is 6×10-14 mol or 15 pg thymine dimer. It is highly specific: A structurally similar compound such as uridine dimer \(\left( {\overline {UU} } \right)\) interferes with the detection of thymine dimers only when it is 53,000-fold or more in molar excess. Since this RIA method does not require the use of labeled DNA, it represents a considerable improvement for repair studies with radiation-sensitive cells.
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Klocker, H., Auer, B., Burtscher, H.J. et al. A sensitive radioimmuno assay for thymine dimers. Mol Gen Genet 186, 475–477 (1982). https://doi.org/10.1007/BF00337950
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DOI: https://doi.org/10.1007/BF00337950