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Regional replication of the bacterial chromosome induced by derepression of prophage lambda

II. Direction and Origin

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Summary

We have demonstrated previously by DNA-DNA hybridization that induction of λ phage with wild type O and P genes results in an increase of bacterial DNA in the chromosomal region adjacent to the left of the prophage, that is a segment between gal and att λ (gal DNA) (Imae and Fukasawa, 1970). Evidence is presented in this report that such an increase of bacterial DNA is also seen in the region to the right of the prophage; a segment between bio and att λ (bio DNA). We postulate therefore that the bidirectional replication of λ DNA extends beyond the prophage and copies the neighboring host DNA until the prophage is excised. The model is verified by making use of excision-defective λ phages. The synthesis of gal DNA (or bio DNA) slows down to a halt within 40 min after the induction in the normal lysogens. The results are attributed to the prophage excision: (1) In lysogens for λint, synthesis of the bacterial DNA continues for longer times. (2) The synthesis of the bacterial DNA slows down to a halt in lysogens for λxis or λb2 as in the control. However λ DNA synthesis also slows down in parallel so that the amount of the bacterial DNA relative to that of λ DNA synthesized by a given time stays constant from 20 min to 80 min. During that time the relative amount of the bacterial DNA rapidly decreases in the normal lysogen.

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References

  • Adhya, S., Echols, H.: Glucose effect and the galactose enzymes of Escherichia coli. Correlation between glucose inhibition of induction and inducer transport. J. Bact. 92, 601–608 (1966)

    Google Scholar 

  • Brooks, K.: Studies in the physiological genetics of some suppressor-sensitive mutants of bacteriophage λ. Virology 26, 489–499 (1965)

    Google Scholar 

  • Buttin, G.: Mécanismes Régulateurs dans la Biosynthèse des Enzymes du Métabolisme du Galactose chez Escherichia coli K12. I. La Biosynthèse Induite de la Galactokinase et l'Induction Simultanée de la Séquence Enzymatique. J. molec. Biol. 7, 164–182 (1963a)

    Google Scholar 

  • Buttin, G.: Mécanismes Régulateurs dans la Biosynthèse des Enzymes du Métabolisme du Galactose chez Escherichia coli K12. II. Le Déterminisme Génétique de la Régulation. J. molec. Biol. 7, 183–205 (1963b)

    Google Scholar 

  • Buttin, G.: Mécanismes Régulateurs dans la Biosynthèse des Enzymes du Métabolisme du Galactose chez Escherichia coli K12. III. L'«Effect de Dérépression» provoqué par le Développement du phage λ. J. molec. Biol. 7, 610–631 (1963c)

    Google Scholar 

  • Buttin, G., Jacob, F., Monod, J.: Synthèse Constitutive de Galactokinase en Conséquence au Développement des Bactériophages λ chez Escherichia coli K12. C.R. Acad. Sci. (Paris) 250, 2471–2473 (1960)

    Google Scholar 

  • Campbell, A.: Sensitive mutants of bacteriophage λ. Virology 14, 22–32 (1961)

    Google Scholar 

  • Denhardt, D.T.: A membrane filter technique for the detection of complementary DNA. Biochem. biophys. Res. Commun. 23, 641–646 (1966)

    Google Scholar 

  • Dove, W.F.: Action of the lambda chromosome. I. Control of functions late in bacteriophage development. J. molec. Biol. 19, 187–201 (1966)

    Google Scholar 

  • Freifelder, D., Kirschner, I., Goldstein, R., Baran, N.: Physical study of prophage excision and curing of λ prophage from lysogenic Escherichia coli. J. molec. Biol. 74, 703–720 (1973)

    Google Scholar 

  • Gottesman, M.E., Yarmolinsky, M.B.: Integration-negative mutants of bacteriophage lambda. J. molec. Biol. 31, 487–505 (1968a)

    Google Scholar 

  • Gottesman, M.E., Yarmolinsky, M.B.: The integration and excision of the bacteriophage lambda genome. Cold Spr. Harb. Symp. quant. Biol. 33, 735–747 (1968b)

    Google Scholar 

  • Guarneros, G., Echols, H.: New mutants of bacteriophage λ with a specific defect in excision from the host chromosome. J. molec. Biol. 47, 565–574 (1970)

    Google Scholar 

  • Hershey, A.D.: Glossary. In: The bacteriophage lambda (Hershey, A.D., ed.), pp. 773–777. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory 1971

    Google Scholar 

  • Hirai, K., Fukasawa, T.: Phage-controlled replication of bacterial chromosomes following derepression of prophage λ. Jap. J. Genet. 46, 416 [abstract, in Japanese] (1971)

    Google Scholar 

  • Hirai, K., Fukasawa, T.: Lambda phage-directed replication of host bacterial chromosome. Genetics 74, s115 (abstract) (1973a)

  • Hirai, K., Fukasawa, T.: Lambdoid phage-induced replication of bacterial chromosome. Seikagaku 45, 472 [abstract, in Japanese] (1973b)

    Google Scholar 

  • Imae, Y., Fukasawa, T.: On the mechanism of derepression of host galactose operon following induction of bacteriophage λ in Escherichia coli strain K12. Biochem. biophys. Res. Commun. 28, 38–43 (1967)

    Google Scholar 

  • Imae, Y., Fukasawa, T.: Regional replication of the bacterial chromosome induced by derepression of prophage lambda. J. molec. Biol. 54, 585–597 (1970)

    Google Scholar 

  • Joyner, A., Isaacs, L.N., Echols, H., Sly, W.S.: DNA replication and messenger RNA production after induction of wild-type λ bacteriophage and λ mutants. J. molec. Biol. 19, 174–186 (1966)

    Google Scholar 

  • Klein, A., Bonhoeffer, F.: DNA replication. Ann. Rev. Biochem. 41, 301–332 (1972)

    Google Scholar 

  • Krell, K., Gottesman, M.E., Parks, J.S., Eisenberg, M.A.: Escape synthesis of the biotin operon in induced λb2 lysogens. J. molec. Biol. 68, 69–82 (1972)

    Google Scholar 

  • Mizuuchi, K., Fukasawa, T.: Chromosome mobilization in rec-merodiploids of Escherichia coli K12 following infection with bacteriophage λ. Virology 39, 467–481 (1969)

    Google Scholar 

  • Ogawa, T., Tomizawa, J.: Replication of bacteriophage DNA. I. Replication of DNA of lambda phage defective in early functions. J. molec. Biol. 38, 217–225 (1968)

    Google Scholar 

  • Packman, S., Sly, W.S.: Constitutive λ DNA replication by λc17, a regulatory mutant related to virulence. Virology 34, 778–789 (1968)

    Google Scholar 

  • Schnös, M., Inman, R.B.: Position of branch points in replicating λ DNA. J. molec. Biol. 51, 61–73 (1970)

    Google Scholar 

  • Seadler, H., Gullon, A., Fiethen, L., Starlinger, P.: Negative control of the galactose operon in E. coli. Molec. gen. Genet. 102, 79–88 (1968)

    Google Scholar 

  • Shulman, M., Gottesman, M.: Lambda att 2: A transducing phage capable of intramolecular int-xis promoted recombination. In: The bacteriophage lambda (Hershey, A.D., ed.), pp. 477–487. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory 1971

    Google Scholar 

  • Skalka, A., Butler, B., Echols, H.: Genetic control of transcription during development of phage λ. Proc. nat. Acad. Sci. (Wash.) 58, 576–583 (1967)

    Google Scholar 

  • Stevens, W.F., Adhya, S., Szybalski, W.: Origin and bidirectional orientation of DNA replication in coliphage lambda. In: The bacteriophage lambda (Hershey, A.D., ed.), pp. 515–533. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory 1971

    Google Scholar 

  • Szybalski, E.H., Szybalski, W.: Physical mapping of the att-N region of coliphage lambda: Apparent oversaturation of coding capacity in the gam-ral segment. Biochimie (Paris) 56, 1497–1503 (1974)

    Google Scholar 

  • Taylor, A.L.: Current linkage map of Escherichia coli. Bact. Rev. 34, 155–175 (1970)

    Google Scholar 

  • Tomizawa, J., Ogawa, T.: Replication of phage λ DNA. Cold Spr. Harb. Symp. Quant. Biol. 33, 533–551 (1968)

    Google Scholar 

  • Yamagishi, H., Skalka, A.: Distribution of nucleotides in Escherichia coli DNA from λ transducing phages. J. molec. Biol. 58, 417–430 (1971)

    Google Scholar 

  • Yarmolinsky, M.B.: Influence of phages on the synthesis of host enzymes of bacteria. In: Viruses, nucleic acids, and cancer, pp. 155–172. Baltimore: Williams & Wilkins Co. 1963

    Google Scholar 

  • Yarmolinsky, M.B., Wiesmeyer, H.: Regulation by coliphage lambda of the expression of the capacity to synthesize a sequence of host enzymes. Proc. nat. Acad. Sci. (Wash.) 46, 1626–1645 (1960)

    Google Scholar 

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Author notes

  1. Kazuko Hirai

    Present address: Department of Nutritional Biochemistry, Osaka City University, Sumiyoshi, Osaka, Japan

  2. Toshio Fukasawa

    Present address: Research Unit of Molecular Genetics, Department of Microbiology, Keto University School of Medicine, Shinjuku, Tokyo, Japan

Authors and Affiliations

  1. Institute for Protein Research, Osaka University, Suita, Osaka, Japan

    Kazuko Hirai & Toshio Fukasawa

Authors
  1. Kazuko Hirai
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  2. Toshio Fukasawa
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Additional information

Communicated by W. Arber

The first article of this series is in J. molec. Biol. 54, 585 (1970).

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Hirai, K., Fukasawa, T. Regional replication of the bacterial chromosome induced by derepression of prophage lambda. Molec. gen. Genet. 147, 71–78 (1976). https://doi.org/10.1007/BF00337938

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  • DOI: https://doi.org/10.1007/BF00337938

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