Molecular and General Genetics MGG

, Volume 189, Issue 2, pp 282–288 | Cite as

Tn2610, a transposon involved in the spread of the carbenicillin-hydrolyzing β-lactamase gene

  • Tomoko Yamamoto
  • Masato Watanabe
  • Kayoko Matsumoto
  • Tetsuo Sawai


We have found a new transposon, Tn2610, on pCS200 in clinical isolates of Escherichia coli, which encodes the carbenicillin-hydrolyzing β-lactamase gene in combination with the resistance determinants to streptomycin and sulfonamide. Tn2610 has a molecular size of 24 kilobase pairs and is flanked by long inverted repeat sequences of 3 kilobase pairs in length. Genetical and physical analyses indicate that Tn2610 is a single transposable unit encoding the multiple resistance determinants and that is different from any previously described transposon. The characteristic DNA structure observed in various complex resistance transposons involved in the transposition of the carbenicillin-hydrolyzing β-lactamase gene is discussed.


Sulfonamide Escherichia Coli Streptomycin Repeat Sequence Clinical Isolate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Alton NK, Vapnek D (1979) Nucleotide sequence analysis of the chloramphenicol resistance transposon Tn9. Nature 282:864–869Google Scholar
  2. Andreoli P, Overbeeke N, Veltkam E, van Embden J, Nijkamp H (1978) Genetic map of the bacteriocinogenic plasmid CLODF13 derived by insertion of the transposon Tn901. Mol Gen Genet 160:1–11Google Scholar
  3. Benedict M, Fennewald M, Shapiro J (1977) Transposition of betalactamase locus from RP1 into Pseudomonas putida derivative plasmid. J Bacteriol 129:809–814Google Scholar
  4. Bennett PM, Richmond MH (1976) The translocation of discrete piece of DNA carrying an amp gene between replicons in Escherichia coli. J Bacteriol 126:1–6Google Scholar
  5. Chang ACY, Cohen SN (1978) Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J Bacteriol 134:1141–1156Google Scholar
  6. Cohen SN (1976) Transposable genetic elements and plasmid evolution. Nature 263:731–738Google Scholar
  7. Datta N, Hedges RW (1972) Trimethoprim resistance conferred by W plasmid in Enterobacteriacea. J Gen Microbiol 72:349–355Google Scholar
  8. Foster TJ, Davis MA, Roberts DE, Takeshita K, Kleckner N (1981) Genetic organization of transposon Tn10. Cell 23:201–213Google Scholar
  9. Hedges RW, Jacob AE (1974) Transposition of ampicillin resistance from RP4 to other replicons. Mol Gen Genet 132:31–40Google Scholar
  10. Hedges RW, Matthew M (1979) Acquisition by Escherichia coli of plasmid-borne β-lactamases normally confined to Pseudomonas spp. Plasmid 2:269–278Google Scholar
  11. Heffron F, Bedinger P, Chanmpoux JJ, Falkow S (1977) Deletions affecting the transposition of an antibiotic resistance gene. Proc Natl Acad Sci USA 69:702–706Google Scholar
  12. Heffron F, Sublett R, Hedges RW, Jacob A, Falkow S (1975) Origin of the TEM beta-lactamase gene found on plasmids. J Bacteriol 122:250–256Google Scholar
  13. Katsu K, Inoue M, Mitsuhasi S (1982) Transposition of the carbenicillin-hydrolyzing beta-lactamase gene. J Bacteriol 150: 483–489Google Scholar
  14. Kleckner N (1981) Transposable elements in prokaryotes. Ann Rev Genet 15:341–404Google Scholar
  15. Kopecko DJ (1980) Specialized genetic recombination systems in bacteria: Their involvement in gene expression and evolution. In: Hahn F (ed), Progress in molecular and subcellular biology. Springer-Verlag, Berlin, Heidelberg, New York, pp 135–234Google Scholar
  16. Matthew M (1979) Plasmid-mediated β-lactamases of Gram-negative bacteria: properties and distribution. J Antimicrobial Chemother 5:349–358Google Scholar
  17. Medeiros AA, Heges RW, Jacoby GA (1982) Spread of “Pseudomonas-specific” β-lactamase to plasmids of enterobacteria. J Bacteriol 149:700–707Google Scholar
  18. Nishimura Y, Caro L, Berg CM, Hirota Y (1971) Chromosome replication in Escherichia coli IV Control of chromosome replication and all division by an integrated episome. J Mol Biol 55:441–456Google Scholar
  19. Perret CJ (1954) Iodometric assay of penicillinase. Nature 174:1012–1013Google Scholar
  20. Richmond MH, Bennett PM, Choi CL, Brown N, Brunton J, Grinsted J, Wallace L (1980) The genetic basis of the spread of β-lactamase synthesis among plasmid-carrying bacteria. Phil Trans R Soc Lond B 289:349–359Google Scholar
  21. Rothstein SJ, Jorgensen RA, Postle K, Rezinikoff WS (1980) The inverted repeats of Tn5 are functionally different. Cell 19:795–805Google Scholar
  22. Tanaka M, Harafuji H, Yamamoto T (1982) A gene and its product required for transposition of resistance transposon Tn2603. J Bacteriol 151:723–728Google Scholar
  23. Watanabe T, Furuse C, Sakaizumi S (1968) Transduction of various R factors by phage P1 in Escherichia coli and by phage P22 in Salmonella typhimurium. J Bacteriol 9:1791–1795Google Scholar
  24. Yamada Y, Calame KL, Grindley N, Nakada D (1979) Location of an ampicillin resistance transposon Tn1701 in a group of small, non-transferring plasmids. J Bacteriol 137:990–999Google Scholar
  25. Yamamoto T, Katoh R, Shimazu A, Yamagishi S (1980) Gene expression of ampicillin resistance transposon, Tn2601 and Tn2602. Microbiol Immunol 24:479–494Google Scholar
  26. Yamamoto T, Tanaka M, Baba R, Yamagishi S (1981a) Physical and functional mapping of Tn2603, a transposon encoding ampicillin, streptomycin, sulfonamide, and mercury resistance. Mol Gen Genet 181:464–469Google Scholar
  27. Yamamoto T, Tanaka M, Nohara C, Fukunaga Y, Yamagishi S (1981b) Transposition of oxacillin-hydrolyzing penicillinase gene. J Bacteriol 145:808–813Google Scholar
  28. Yun T, Vapnek D (1977) Structure and location of antibiotic resistance determinants in bacteriophage P1Cm and P7 (ϕamp). In: Bukhari A, Shapiro JA, Adhya S (eds) DNA insertion elements, plasmids, and episomes. Cold Spring Harbor Press, Cold Spring Harbor NY, pp 229–234Google Scholar

Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • Tomoko Yamamoto
    • 1
  • Masato Watanabe
    • 1
  • Kayoko Matsumoto
    • 1
  • Tetsuo Sawai
    • 1
  1. 1.Division of Microbial Chemistry, Faculty of Pharmaceutical SciencesChiba UniversityChibaJapan

Personalised recommendations