Summary
Drosophila melanogaster flies were transformed with a yp1-Adh fusion gene with 890 bp of yp1 5′ flanking sequence. In an Adh - background these flies show a stage, tissue and sex-specific pattern of alcohol dehydrogenase (ADH) activity characteristic of yolk protein genes. ADH activity is not present in dsx D/dsx pseudomales indicating that this fragment contains sites where the dsx gene product exerts its effect. Transformed male flies do not exhibit ADH activity when injected with 20-hydroxyecdysone while synthesis of native yolk proteins is induced. Thus the hormone inducibility and sex regulation have been separated in this construct.
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Shirras, A.D., Bownes, M. Separate DNA sequences are required for normal female and ecdysone-induced male expression of Drosophila melanogaster yolk protein 1. Mol Gen Genet 210, 153–155 (1987). https://doi.org/10.1007/BF00337772
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DOI: https://doi.org/10.1007/BF00337772