Summary
Somatic hybridization of rice (Oryza sativa L.) and barnyard grass (Echinochloa oryzicola), a close relative of barnyard millet, was attempted using electrofusion and a new culture method developed for rice protoplasts (Kyozuka et al. 1987) to incorporate some of the agronomically important characters of the latter species into rice. Selection of hybrids was based on inactivation of rice protoplasts by iodoacetamide and the inability of barnyard grass protoplasts to divide. A total of 166 calli were identified as hybrids by isozyme and chromosome analyses. Hybrid calli were highly morphogenic, and 44 shoots were obtained. Most of them, however, were abnormal, and nine grew to plantlets whose morphology was distinct from that of either parent. Our study clearly demonstrates the totipotency of protoplasts in graminaceous monocots.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Abdullah R, Cocking EC, Thompson JA (1986) Efficient plant regeneration from rice protoplasts through somatic embryogenesis. Bio/Technology 4:1087–1090
Belliard GF, Vedel F, Pelletier G (1979) Mitochondrial recombination in cytoplasmic hybrids of Nicotiana tabacum by protoplast fusion. Nature 281:401–403
Boeshore M, Lifshitz I, Hanson MR, Izhar S (1983) Novel composition of mitochondrial genomes in Petunia somatic hybrids derived from cytoplasmic male sterile and fertile plants. Mol Gen Genet 190:459–467
Chen TM, Brown RH, Black CC (1970) CO2 compensation concentration, rate of photosynthesis, and carbonic anhydrase activity of plants. Weed Sci 18:399–403
Chu CC, Wang CC, Sun CS, Hsu C, Yin KC, Chu CY, Bi FY (1975) Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Sci Sin [B] 18:659–668
Coulibaly MY, Demarly Y (1986) Regeneration of plantlets from protoplasts of rice, Oryza sativa L. Z Pflanzenzucht 96:79–81
Fujimura T, Sakurai M, Akagi H, Negishi T, Hirose A (1985) Regeneration of rice plants from protoplasts. Plant Tissue Culture Lett 2:74–75
Gleba YY, Hoffmann F (1980) “Arabidobrassica”: A novel plant obtained by protoplast fusion. Planta 149:112–117
Kuroiwa T, Kawano S, Nishibayashi S, Sato C (1982) Epifluorescent microscopic evidence for maternal inheritance of chloroplast DNA. Nature 298:481–483
Kyozuka J, Hayashi Y, Shimamoto K (1987) High frequency plant regeneration from rice protoplasts by novel nurse culture methods. Mol Gen Genet 206:408–413
Medgesy P, Fejes E, Maliga P (1985) Interspecific chloroplast recombination in a Nicotiana somatic hybrid. Proc Natl Acad Sci USA 82:6960–6964
Melchers G, Sacristán MD, Holder AA (1978) Somatic hybrid plants of potato and tomato regenerated from fused protoplasts. Carlsberg Res Commun 43:203–218
Menczel L, Wolfe K (1984) High frequency of fusion induced in freely suspended protoplast mixtures by polyethylene glycol and dimethylsulfoxide at high pH. Plant Cell Rep 3:196–198
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Ozias-Akins P, Ferl RJ, Vasil IK (1986) Somatic hybridization in the gramineae: Pennisetum americanum (L.) K. Schum. (pearl millet) + Panicum maximum Jacq. (Guinea grass). Mol Gen Genet 203:365–370
Tabaeizadeh Z, Ferl RJ, Vasil IK (1986) Somatic hybridization in the Gramineae. Saccharum officinarum L. (Sugarcane)+ Pennisetum americanum (L.) K. Schum. (Pearl millet). Proc Natl Acad Sci USA 83:5616–5619
Takahashi A, Sakuragi Y, Kamada H, Ishizuka K (1984) Plant regeneration through somatic embryogenesis in barnyardgrass, Ecinochloa oryzicola Vasing. Plant Sci Lett 36:161–163
Tateoka T (1957) Miscellaneous papers on the phylogeny of Poaceae (10). Proposition of a new phylogenic system of Poaceae. J Japan Bot 32:275–287
Terada R, Yamashita Y, Nishibayashi S, Shimamoto K (1987) Somatic hybrids between Brassica oleracea and B. campestris: selection by the use of iodoacetamide inactivation and regeneration ability. Theor Appl Genet 73:379–384
Toriyama K, Hinata K, Sasaki T (1986) Haploid and diploid plant regeneration from protoplasts of anther callus in rice. Theor Appl Genet 73:16–19
Vallejos CE (1983) Enzyme activity staining. In: Tanksley SD, Orton TJ (eds) Isozymes in plant genetics and breeding. Part A. Elsevier Science Publishers, Amsterdam, pp 469–516
Yamada Y, Yang ZQ, Tang DT (1986) Plant regeneration from protoplast-derived callus of rice (Oryza sativa L.). Plant Cell Rep 4:85–88
Author information
Authors and Affiliations
Additional information
Communicated by G. Melchers
Rights and permissions
About this article
Cite this article
Terada, R., Kyozuka, J., Nishibayashi, S. et al. Plantlet regeneration from somatic hybrids of rice (Oryza sativa L.) and barnyard grass (Echinochloa oryzicola Vasing). Mol Gen Genet 210, 39–43 (1987). https://doi.org/10.1007/BF00337756
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00337756