Summary
The cell division cycle gene CDC25 was replaced by various disrupted and deleted mutant copies. Mutants disrupted at a central position of the gene, or lacking 532 residues within the amono-terminal half of the gene product grow normally in glucose, but not in acetate media, and they fail to sporulate as homozygous diploids. Disruptions or deletions within the carboxy-terminal half are lethal, except for the deletion of the 38 carboxy-terminal residues, which are required for sporulation but not for growth in glucose or acetate media. It is concluded that distinct domains of the CDC25 gene product are involved in the control of mitosis and/or meiosis.
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Broek D, Toda T, Michaeli T, Levi L, Birchmeier C, Zoller M, Powers S, Wigler M (1987) The S. cerevisiae CDC25 gene product regulates the RAS/adenylate cyclase pathway. Cell 48:789–799
Camonis JH, Kalékine M, Gondré B, Garreau H, Boy-Marcotte E, Jacquet M (1986) Characterization, cloning and sequence analysis of the CDC25 gene which controls the cyclic AMP level of Saccharomyces cerevisiae. EMBO J 5:375–380
Cannon JF, Tatchell K (1987) Characterization of Saccharomyces cerevisiae genes encoding subunits of cyclic AMP-dependent protein kinase. Mol Cell Biol 7:2653–2663
Carle GF, Olson MV (1984) Separation of chrosomal DNA molecules from yeast by orthogonal-field-alternation gel electrophoresis. Nucleic Acids Res 12:5647–5664
Daniel J, Simchen G (1986) Clones from two different genomic regions complement the cdc25 start mutation of Saccharomyces cerevisiae. Curr Genet 10:643–646
Davis CG, Elhammer A, Russell DW, Schneider WJ, Kornfeld S, Brown MS, Goldstein JL (1986) Deletion of clustered Olinked carbohydrates does not impair function of low-density lipoprotein receptor in transfected fibroblasts. J Biol Chem 261:2828–2838
Hartwell LH (1974) Saccharomyces cerevisiae cell cycle. Bacteriol Rev 38:164–198
Kataoka T, Powers S, McGill C, Fasano O, Strathern J, Broach J, Wigler M (1984) Genetic analysis of yeast RAS1 and RAS2 genes. Cell 37:437–445
Küntzel H, Lisziewicz J, Godany A, Hostinova E, Förster HH, Trauzold M, Sternbach H (1987) Control of the cell cycle start by protein kinase genes in Saccharomyces cerevisiae. In: Zelinka L, Balan J (eds) Metabolism and enzymology of nucleic acids including gene manipulations. Slovak Academy of Sciences, Bratislava, pp 199–203
Kyte J, Doolittle RF (1982) A simple method for displaying the hydrophathic character of a protein. J Mol Biol 157:105–132
Lisziewicz J, Godany A, Förster HH, Küntzel H (1987) Isolation and nucleotide sequence of a Saccharomyces cerevisiae protein kinase gene suppressing the cell cycle start mutation cdc25. J Biol Chem 262:2549–2553
Madaule P, Axel R, Myers AM (1987) Characterization of two members of the rho gene family from the yeast Saccharomyces cerevisiae. Proc Natl Acad Sci USA 84:779–783
Marshall MS, Gibbs JB, Scolnick EM, Sigal IS (1987) Regulatory function of the Saccharomyces cerevisiae RAS C-terminus. Mol Cell Biol 7:2309–2315
Martegani E, Baroni MD, Frascotti G, Alberghina L (1986) Molecular cloning and transcriptional analysis of the start gene CDC25 of Saccharomyces cerevisiae. EMBO J 5:2363–2369
Matsumoto K, Uno I, Ishikawa T (1983) Initiation of meiosis in yeast mutants defective in adenylate cyclase and cyclic AMP-dependent protein kinase. Cell 32:417–423
Nikawa J, Cameron S, Toda T, Ferguson KM, Wigler M (1987) Rigorous feedback control of cAMP levels in Saccharomyces cerevisiae. Genes and Development 1:931–937
Pohlig G, Holzer H (1985) Phosphorylation and inactivation of yeast fructose-1,6-bisphosphatase by cyclic AMP-dependent protein kinase from yeast. J Biol Chem 20:13818–13823
Robinson LC, Gibbs JB, Marshall MS, Sigal IS, Tatchell K (1987) CDC25: a component of the RAS-adenylate cyclase pathway in Saccharomyces cerevisiae. Science 235:1218–1221
Schmitt HD, Wagner P, Pfaff E, Gallwitz D (1986) The ras-related YPT1 gene product in yeast: a GTP-binding protein that might be involved in microtubule organization. Cell 47:401–412
Segev N, Botstein D (1987) The ras-like yeast YPT1 gene is itself essential for growth, sporulation, and starvation response. Mol Cell Biol 7:2367–2377
Sherman F, Fink GR, Hicks JB (1982) Methods in yeast genetics, laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
Shilo V, Simchen G, Shilo B (1978) Initiation of meiosis in cell cycle initiation mutants of Saccharomyces cerevisiae. Exp Cell Res 112:241–248
Struhl K, Stinchcombe DT, Scherer S, Davis RW (1979) Highfrequency transformation of yeast: autonomous replication of hybrid DNA. Proc Natl Acad Sci USA 76:1035–1039
Tatchell K, Chaleff D, Defeo-Jonas D, Scolnick E (1984) Requirement of either of a pair of ras related genes of Saccharomyces cerevisiae for spore viability. Nature 309:523–527
Tatchell K, Robinson LC, Breitenbach M (1985) RAS2 of Saccharomyces cerevisiae is required for gluconeogenetic growth and proper response to nutrient limitation. Proc Natl Acad Sci USA 82:3785–3789
Toda T, Uno I, Ishikawa T, Powers S, Kataoka T, Broek D, Cameron S Broach J, Matsumoto K, Wigler M (1985) In yeast, RAS proteins are controlling elements of adenylate cyclase. Cell 40:27–36
Toda T, Cameron S, Sass P, Zoller M, Wigler M (1987a) Three different genes in S. cerevisiae encode the catalytic subunits of the cAMP-dependent protein kinase. Cell 50:277–287
Toda T, Cameron S, Sass P, Zoller M, Scott JD, McMullen B, Hurwith M, Krebs EG, Wigler M (1987b) Cloning and characterization of BCY1, a locus encoding a regulatory subunit of the cyclic AMP-dependent protein kinase in Saccharomyces cerevisiae. Mol Cell Biol 7:1371–1377
Uno I, Matsumoto K, Adachi K, Ishikawa T (1983) Genetic and biochemical evidence that trehalase is a substrate of cAMP-dependent protein kinase in yeast. J Biol Chem 258:10867–10872
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Communicated by C.P. Hollenberg
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Munder, T., Mink, M. & Küntzel, H. Domains of the Saccharomyces cerevisiae CDC25 gene controlling mitosis and meiosis. Mol Gen Genet 214, 271–277 (1988). https://doi.org/10.1007/BF00337721
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DOI: https://doi.org/10.1007/BF00337721