, Volume 100, Issue 1, pp 37–47

The puff-specific Drosophila protein Bj6, encoded by the gene no-on transient A, shows homology to RNA-binding proteins

  • Hans von Besser
  • Petra Schnabel
  • Claudia Wieland
  • Elke Fritz
  • Ralf Stanewsky
  • Harald Saumweber
Chromosoma Focus


The DNA coding for the puff-specific protein Bj6 has been isolated by expression cloning. The gene is localized in 14C1,2 on the X chromosome and is expressed ubiquitously during embryonic development with prominent expression during the first 12 h of embryogenesis. cDNA and genomic clones have been sequenced and show a single open-reading frame of 2.1 kb length, coding for a Mr=77000 basic protein. In the aminoterminal half of the protein we detect stretches of repeated amino acids, centrally a region with homology to RNA-binding proteins containing the RNP 1 and RNP 2 consensus motif of RNA binding proteins, and the carboxyterminal part is rich in charged amino acids. The Bj6 protein is a product of the gene no-on transient A, a gene required for normal vision and courtship behaviour in Drosophila.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. BandziulisRJ, SwansonMS, DreyfussG (1989) RNA-binding proteins as developmental regulators. Gen Dev 3: 431–437Google Scholar
  2. BialojanS, FalkenburgD, Renkawitz-PohlR (1984) Characterization and developmental expression of β tubulin genes in Drosophila melanogaster. EMBO J 3: 2543–2548Google Scholar
  3. BirnstielML, BusslingerM, StrubK (1985) Transcription termination and 3′-processing: the end is in site! Cell 41: 349–359Google Scholar
  4. BrethnachR, ChambonP (1981) Organization and expression of eukaryotic split genes coding for proteins. Ann Rev Biochem 50: 349–383Google Scholar
  5. Campos-OrtegaJA, HartensteinV (1985) The embryonic development of Drosophila melanogaster. Springer, Berlin Heidelberg New York TokyoGoogle Scholar
  6. ChirgwinJM, PrzybylaAE, Mac DonaldRJ, RutterWJ (1979) Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18: 5294–5299Google Scholar
  7. FeinbergAP, VogelsteinB (1984) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 137: 266–267Google Scholar
  8. Frasch M (1985) Charakterisierung chromatinassoziierter Kernproteine von Drosophila melanogaster mit Hilfe monoklonaler Antikörper. Dissertation, University of TübingenGoogle Scholar
  9. FraschM, SaumweberH (1989) Two proteins from Drosophila nuclei are bound to chromatin and are detected in a series of puffs on polytene chromosomes. Chromosoma 97: 272–281Google Scholar
  10. HeisenbergM (1971) Isolation of mutants lacking the optomotor response Dros Inf Serv 46: 68Google Scholar
  11. HeisenbergM (1972) Comparative behavioral studies on two visual mutants of Drosophila. J Comp Physiol 80: 119–136Google Scholar
  12. HeisenbergM, GötzKG (1975) The use of mutations for the partial degradation of vision in Drosophila melanogaster. J Comp Physiol 98: 217–241Google Scholar
  13. HottaY, BenzerS (1970) Genetic dissection of the Drosophila nervous system by means of mosaics. Proc Natl Acad Sci USA 67: 1156–1163Google Scholar
  14. InghamPW, HowardKR, Ish-HorowiczD (1985) Transcription pattern of the Drosophila segmentation gene hairy. Nature 318: 439–444Google Scholar
  15. JonesKR, RubinGM (1990) Molecular analysis of no-on-transient A, a gene required for normal vision in Drosophila. Neuron 4: 711–723Google Scholar
  16. JowettT (1986) Preparation of nucleic acids. In: RobertsDR (ed) Drosophila—a practical approach. IRL Press, Oxford Washington, pp 275–277Google Scholar
  17. KulkarniSJ, SteinlaufAF, HallJC (1988) The dissonance mutant of courtship song in Drosophila melanogaster: isolation, behavior and cytogenetics. Genetics 118: 267–285Google Scholar
  18. Langer-SaferPR, LevineM, WardDC (1982) Immunological method for mapping genes on Drosophila polytene chromosomes. Proc Natl Acad Sci USA 79: 4381–4385Google Scholar
  19. LipmanDJ, PearsonWR (1985) Rapid and sensitive protein similarity searches. Science 227: 1435–1441Google Scholar
  20. ManiatisT, FritschEF, SambrookJ (1982) Molecular cloning—a laboratory manual. Cold Spring Harbor Laboratory, New YorkGoogle Scholar
  21. McKnightSL, KingsburyR (1982) Transcriptional control signals of a eukaryotic protein-coding gene. Science 217: 316–324Google Scholar
  22. MountSM (1982) A catalogue of splice junction sequences. Nucl Acids Res 10: 459–472Google Scholar
  23. PirottaV, HadfieldC, PretoriusGHK (1983) Microdissection and cloning of the white locus and the 3B1-3C2 region of the Drosophila X chromosome. EMBO J 2: 927–934Google Scholar
  24. PooleSJ, KauvarLM, DreesB, KornbergT (1985) The engrailed Locus of Drosophila: structural analysis of an embryonic transcript. Cell 40: 37–43Google Scholar
  25. ReedKC, MannDA (1985) Rapid transfer of DNA from agarose gels to nylon membranes. Nuclei Acids Res 13: 7207–7221Google Scholar
  26. RemautE, TsaoH, FiersW (1983) Improved plasmidvectors with thermoinducible expression and temperature—regulated runaway replication. Gene 22: 103–113Google Scholar
  27. RenanMJ (1987) Conserved 12-bp element downstream from mRNA polyadenylation sites. Gene 60: 245–254Google Scholar
  28. RisauW, SymmonsP, SaumweberH, FraschM (1983) Nonpackaging and packaging proteins of hnRNA in Drosophila melanogaster. Cell 33: 529–541Google Scholar
  29. SachsAB, BondWM, KornbergR (1986) A single gene from yeast for both nuclear and cytoplasmic polyadenylate-binding protein: domain structure and expression. Cell 45: 827–835Google Scholar
  30. SambrookJ, FritschEF, ManiatisT (1989) Molecular cloning—a laboratory manual. Cold Spring Harbor Laboratory, New YorkGoogle Scholar
  31. SangerF, CoulsonAR, BarellBG, SmithAJ, RoeBA (1980) Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing. J Mol Biol 143: 161–178Google Scholar
  32. SaumweberH, SymmonsP, KabischR, WillH, BonhoefferF (1980) Monoclonal antibodies against chromosomal proteins of Drosophila melanogaster. Chromosoma 80: 253–275Google Scholar
  33. SaumweberH, FraschM, KorgeG (1990) Two puff-specific proteins bind within the 2.5 kb upstream region of the Drosophila melanogaster Sgs-4 gene. Chromosoma 99: 52–60Google Scholar
  34. SchaletAP (1986) Distribution of and complementation relationships between spontaneous X-linked recessive lethal mutations recovered from crossing long-term laboratory stocks of Drosophila melanogaster. Mut Res 163: 115–144Google Scholar
  35. SchlichtHJ, KuhnC, GuhrB, MattalianoRJ, SchallerH (1987) Biochemical and immunological characterization of the duck hepatitis B virus envelope protein. J Virol 61: 2280–2285Google Scholar
  36. Shippen-LentzD, BlackburnEH (1990) Functional evidence for an RNA template in telomerase. Science 247: 546–552Google Scholar
  37. StrebelK, BeckE, StrohmaierK, SchallerH (1986) Characterization of foot-and mouth disease virus gene products with antisera against bacterially synthesized fusion proteins. J Virol 57: 983–991Google Scholar
  38. SurdejP, GotC, MiassodR (1989) Developmental expression pattern of a 800 kb DNA continuum cloned from Drosophila X-chromosome 14B–15B-region. Biol Cell 68: 105–118Google Scholar
  39. Symmons P (1984) A methodological investigation of production, purification and assay of monoclonal and polyclonal antibodies and their utilisation for quantitative, comparative and functional analysis of componenets of the eucaryotic nucleus. PhD thesis, Eberhard Karls Universität, TübingenGoogle Scholar
  40. WhitfieldW, MillarS, SaumweberH, FraschM, GloverD (1988) Cloning of a gene encoding an antigen assiciated with the centrosome in Drosophila. J Cell Sci 89: 467–480Google Scholar
  41. YoungRA, DavisRW (1983) Efficient isolation of genes by using antibody probes. Proc Natl Acad Sci USA 80: 1194–1198Google Scholar
  42. Yanisch-PerronC, VieiraJ, MessingJ (1984) Improved M13 phage cloning and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33: 103–119Google Scholar

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Hans von Besser
    • 1
  • Petra Schnabel
    • 1
  • Claudia Wieland
    • 1
  • Elke Fritz
    • 1
  • Ralf Stanewsky
    • 1
  • Harald Saumweber
    • 1
  1. 1.Institut für Entwicklungsphysiologie Universität zu KölnKöln 41Federal Republic of Germany

Personalised recommendations