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Localization of acetylated histone H4 in the macronucleus of Euplotes

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Abstract

Antisera specific to acetylated and unacetylated N-terminal domains of histone H4 were employed to map the spatial distribution of exposed antigenic determinants in the macronucleus of Euplotes eurystomus. Sites of putative transcription-related acetylation appear to reside in the regions between condensed chromatin granules and disappear during chromatin restructuring in the forward zone of replication bands. Coincident with synthesis of newly replicated chromatin, the specific antisera reveal a resumption of exposed sites of H4 acetylation in the rear zone of replication bands.

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Authors and Affiliations

  1. The University of Tennessee-Oak Ridge Graduate School of Biomedical Sciences and the Biology Division, Oak Ridge National Laboratory, P.O. Box 2009, 37831-8077, Oak Ridge, TN, USA

    Donald E. Olins, Ada L. Olins & Adria Herrmann

  2. Department of Biology, Syracuse University, 13244-1220, Syracuse, NY, USA

    Rueyling Lin & C. David Allis

  3. Max Planck Institute for Biophysical Chemistry, Postfach 2841, W-3400, Göttingen, Federal Republic of Germany

    Michel Robert-Nicoud

  4. Erfmg Cermo, Université J. Fourier, BP 53x, F-38041, Grenoble, France

    Michel Robert-Nicoud

Authors
  1. Donald E. Olins
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  2. Ada L. Olins
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  3. Adria Herrmann
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  4. Rueyling Lin
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  5. C. David Allis
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  6. Michel Robert-Nicoud
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by L. Manuelidis

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Olins, D.E., Olins, A.L., Herrmann, A. et al. Localization of acetylated histone H4 in the macronucleus of Euplotes . Chromosoma 100, 377–385 (1991). https://doi.org/10.1007/BF00337516

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  • DOI: https://doi.org/10.1007/BF00337516

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