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Genetic analysis of an alteration of ribosomal protein S20 in revertants of an alanyl-tRNA-synthetase mutant of Escherichia coli

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Summary

The genetic location has been determined of two mutations which suppress the temperature-sensitive phenotype of an alanyl-tRNA-synthetase mutant of Escherichia coli and which are correlated with alterations of the ribosomal protein S20. Both mutations map at the same chromosomal site; the gene order relative to other markers of the Escherichia coli map is thr-sup-pyrA-araC-leu.

Replacement of the suppressor allele by the wild-type allele via P1 transduction results in the appearance of the wild-type S20 protein; concomitantly suppression of temperature-sensitivity is released.

Strains of Escherichia coli were contructed which are partially diploid for the region of the chromosome containing the suppressor allele. Investigation of these strains revealed that the wild-type suppressor is dominant as judged by the activity to suppress the alaS mutation since the partial diploids are no longer able to suppress the alaS-3 mutation. Investigation of the ribosomal protein pattern of these partial diploids by means of two-dimensional polyacrylamide gel electrophoresis did not reveal two distinct spots characteristic for the normal and the altered forms of S20; rather, an elongated spot was observed trailing from the wild-type S20 position towards the anode.

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Authors and Affiliations

  1. Lehrstuhl für Mikrobiologie im Fachbereich Biologie der Universität Regensburg, Regensburg, Germany

    A. Böck, D. Ruffler & W. Piepersberg

  2. Max-Planck-Institut für Molekulare Genetik, Berlin-Dahlem

    H. G. Wittmann

Authors
  1. A. Böck
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  2. D. Ruffler
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  3. W. Piepersberg
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  4. H. G. Wittmann
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Communicated by E. Bautz

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Böck, A., Ruffler, D., Piepersberg, W. et al. Genetic analysis of an alteration of ribosomal protein S20 in revertants of an alanyl-tRNA-synthetase mutant of Escherichia coli . Molec. gen. Genet. 134, 325–332 (1974). https://doi.org/10.1007/BF00337467

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  • DOI: https://doi.org/10.1007/BF00337467

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