Summary
Pot experiments with oats were carried out to study the effect of Azospirillum brasilense Sp 7 and Azotobacter chroococcum 94K on the yield of plants, the N content of soil and the 14N balance. The plants were grown on gray forest soil under irrigation with deionized water and application of 15N-labelled fertilizer at a rate of 4 mg N 100 g-1 soil. Inoculation of plants with Azospirillum spp. and Azotobacter spp. failed to increase the plant yield. However, the increase in total N in the soil at the end of the experiment and the positive 14N balance in the soil-plant system due to increased nitrogenase activity in the rhizosphere were statistically significant. The amount of N accumulated in the soil was comparable with the rate of N applied as fertilizer.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Albrecht SL, Okon Y, Lonnquist J, Burris RH (1981) Nitrogen fixation by corn-Azospirillum associations in a temperate climate. Crop Sci 21:301–306
App AA, Watanabe I, Alexander M, Ventura W, Daez C, Santiago T, De Datta SK (1980) Nonsymbiotic nitrogen fixation associated with the rice plant in flooded soils. Soil Sci 130:283–289
Azam F, MalikKA, Sajjad MI (1986) Uptake by wheat plant and turnover within soil fractions of residual N from leguminous plant material and inorganic fertilizer. Plant and Soil 95:97–108
Barber LE, Tjepkema JD, Russell SA, Evans HJ (1976) Acetylene reduction (nitrogen fixation) associated with corn inoculated with Spirillum. Appl Environ Microbiol 32:108–113
Barber LE, Russell SA, Evans HJ (1979) Inoculation of millet with Azospirillum. Plant and Soil 52:49–57
Boddey RM, Döbereiner J (1982) Association of Azospirillum and other diazotrophs with tropical Gramineae. In: Trans 12th Int Congr Soil Sci, New Delhi, vol 1, pp 28–47
Boddey RM, Baldani VLD, Baldani JI, Döbereiner J (1986) Effect of inoculation of Azospirillum ssp on nitrogen accumulation by field-grown wheat. Plant and Soil 95:109–121
Chebotar VK (1985) Responsiveness of different varieties of sorghum to inoculation with root nitrogen fixers. Bull All-Union Sci Res Inst Agric Microbiol 41:6–9
Chistyakova IK, Miller YuM, Kalininskaya TA (1982) Application of 15N to study nitrogen transformation in the soil-plant system. In: Ecological aspects of application of agrochemicals (fertilizers). ON-TI NCBI, Pushchino, USSR, pp 43–44
Dart PJ, Wani SP (1982) Non-symbiotic nitrogen fixation and soil fertility. In: Trans 12th Int Congr Soil Sci, New Delhi. Symp pap 1, pp 3–27
Kapulnik Y, Okon Y, Henis Y (1987) Yield response of spring wheat cultivars (Triticum aestivum and T. turgidum) to inoculation with Azospirillum brasilense under field conditions. Biol Fertil Soils 4:27–35
Kler DS, Chahal VPS (1986) Growth and yield of barley (Hordeum vulgare L.) as affected by Azotobacter chroococcum. Trans Indian Soc Desert Technol Univ Cent Desert Stud 11:33–36
Kozhemyakov AP (1986) The use of 15N isotope technique to study associative nitrogen fixation. In: Minejev VG (ed) Abstracts of the All-Union Conference in the Leningrad State University (USSR), 2, p 56
Kralova M, Kubat J (1976) Tracer studies on nitrogen mineralization-immobilization relationship. Newslett Appl Nucl Methods Biol Agric 7:17–18
Kudeyarov VN (1972) To the method of determination of total nitrogen in soils and plants. Agrokhimiya (USSR) 11:121–125
Lima E, Boddey RM, Döbereiner J (1987) Quantification of biological nitrogen fixation associated with sugar cane using a 15N aided nitrogen balance. Soil Biol Biochem 19:165–170
Lukin SA, Kozhevin PA, Zvyagintsev DG (1987) Azospirillum and associative nitrogen fixation by non-legumes in agriculture. Agric Biol (USSR) 1:51–58
Mamchenkov IP, Tashcheva RM (1972) The effect of organic substances on transformation of nitrogen compounds in soil. In: Mamchenkov IP (ed) Organic manures. Moscow, pp 309–326
Martinez-Toledo MV, Gonzalez-Lopez J, de la Rubia T, Moreno J, Ramos-Cormenzana A (1988) Grain yield response of Zea mays (hybrid AE 703) to Azotobacter chroococcum H23. Biol Fertil Soils 6:352–353
Mishustin EN, Shilnikova VK (1968) Biological fixation of atmospheric nitrogen. Nauka Press, Moscow
Okon Y (1984) The physiology of azospirillum in relation to its utilization as inoculum for promoting growth of plants In: Ludden PW, Burris JE (eds) Proc 14th Steenbock Symposium on Nitrogen fixation. USA. Elsevier Science Publishing, New York Amsterdam Oxford, pp 165–174
Rubenchik LI (1960) Azotobacter and its use in agriculture. Academy of Sciences of Ukrainian SSR Publishers, Kiev
Shabaev VP (1986) The effect of cropping and fertilizer nitrogen rates on nitrogen balance in soil. Plant and Soil 91:249–256
Singh AL, Singh PK (1987) Nitrogen fixation and balance studies of rice soil. Biol Fertil Soils 4:15–19
Sirota LB (1988) The effect of root diazotrophs on the yield and nitrogen uptake by rice at application of different rates of 15NH4 15NO3. In: Gamsikov GP (ed) The application of 15N in agrochemical investigations. Nauka Press, Novosibirsk, pp 127–129
Smith RL, Schank SC, Milam JR, Baltensperger AA (1984) Responses of Sorghum and Pennisetum species to the N2-fixing bacterium Azospirillum brasilense. Appl Microbiol 47:1331–1336
Trolldenier G (1987) Estimation of associative nitrogen fixation in relation to water regime and plant nutrition in a long-term pot experiment with rice (Oryza sativa L.). Biol Fertil Soils 5:133–140
Umarov MM (1976) Acetylene technique for the determination of nitrogen fixation in soil microbiological research. Pochvovedenie (USSR) 11:119–123
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Shabaev, V.P., Smolin, V.Y. & Strekozova, V.I. The effect of Azospirillum brasilense Sp7 and Azotobacter chroococcum on nitrogen balance in soil under cropping with oats (Avena sativa L.). Biol Fert Soils 10, 290–292 (1991). https://doi.org/10.1007/BF00337381
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00337381