Summary
Fetal Pancreas cells of man, whether in “self-aggregation” or “rotation-mediated-aggregation”, form spontaneously histotypical aggregates containing A- and B-cells. It is possible by means of an immunological method to determine “insulin” (=IRI=immuno-reactive-insulin) in the nutrient medium of these 6 to 17 days old tissue aggregates.
Zusammenfassung
Fetale menschliche Pankreaszellen bilden sowohl spontan auf zuerst gewachsenem Zellrasen („self-aggregation“) als auch in Rollröhrchen (48 rph) („rotation-mediated-aggregation“) histotypische Aggregate, die A- und B-Zellen enthalten. In der Nährlösung dieser 6–17 Tage lang gewachsenen Gewebestückchen konnte, „Insulin“ (=IRI=immunoreactive-insulin) nachgewiesen werden.
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Hilwig, I., Schuster, S. Über das Wachstum der Pankreaszellen von Säugetieren als Monolayer Cultures. Z. Zellforsch. 103, 282–290 (1970). https://doi.org/10.1007/BF00337318
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DOI: https://doi.org/10.1007/BF00337318