Summary
In healing skin wounds of rat skin an initial disappearance of mast cells occurs. This is followed by a reappearance and degranulation of mast cells at the wound margins between the third and seventh post operative day. By fourteen days the mast cell distribution around the scar tissue resembles that of normal skin, and a few mast cells are present in the fibrous tissue.
The presence of mast cells exhibiting a pale metachromasia and fewer granules is noted. These cells lay under the stratum germinativum of the epidermis in normal skin, and similar cells are present below areas of epithelial regeneration in the healing wounds.
It is suggested that the mast cell is playing an essential part in wound healing, and that the subepithelial forms may be involved in providing a stimulus for cell division.
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References
Asboe-Hansen, G.: Hormonal effects on connective tissue. Physiol. Rev. 38, 446–462 (1958).
—: Endocrine control of connective tissue. Inflammation and diseases of connective tissue (L. C. Mills, and J. H. Moyer ed.), p. 38–43. Philadelphia: W. B. Saunders Co 1961.
Benditt, E. P., and D. Lagunoff: The mast cell: its structure and function. Progr. Allergy 8, 195–223 (1964).
Bridges, J. B., T. R. Johnson, and H. W. Whitting: Peritoneal adhesion formation. Acta anat. (Basel) (in press).
-, H. W. Whitting: Unpublished results.
Broderson, J.: Die Zellen des Unterhautbindegewebes der weißen Maus. Z. Zellforsch. 14, 60–72 (1928).
Crabb, E. D., and M. A. Kelsall: Relation of cortisone-induced lymphopenia to transplanted sarcoma in hamsters. J. nat. Cancer Inst. 12, 91–97 (1951).
Duff, G. L.: Diffuse collagen diseases: morphological correlation. Canad. med. Ass. J. 58, 317–325 (1948).
Hadfield, G.: The slough covering the surface of an experimental skin wound. Brit. J. Surg. 50, 649–661 (1963a).
—: The earlier phases of definitive healing in an experimental skin wound. Brit. J. Surg. 50, 751–764 (1963b).
—: The tissue of origin of fibroblasts of granulation tissue. Brit. J. Surg. 50, 870–881 (1963c).
Higginbotham, R. D.: Round table discussion. Ann. N. Y. Acad. Sci. 103, 468–469 (1963).
—, T. F. Dougherty, and W. S. S. Jee: Fate of shed mast cell granules. Proc. Soc. exp. Biol. (N. Y.) 92, 256–261 (1956).
Kahlson, G., K. Nilsson, and B. Zederfeld: Wound healing as dependent on the rate of histamine formation. Lancet 1960 II, 230–234.
Kapeller-Adler, R.: Histamine (G. E. W. Wolstenholm and C. M. O'connor, ed.), p. 356–380. Boston: Little, Brown & Co. 1956.
Kelsall, M. A.: Trephocytic activity of lymphocytes, plasmacytes and mast cells in relation to the metabolism of connective tissue. Inflammation and diseases of connective tissue (L. C. Mills and J. H. Moyer, ed.), p. 20–27. Philadelphia: W. B. Saunders Co. 1961.
—, E. D. Crabb: Lymphocytes and plasmacytes in nucleoprotein metabolism. Ann. N. Y. Acad. Sci. 72, 293–337 (1958).
Macleod, C. M.: Cellular and humoral aspects of hypertensive states (H. S. Lawrence, ed.), p. 615–627. New York: Paul B. Hoeber, Inc. 1959.
Maximow, A.: Über entzündliche Bindegewebsneubildung bei der weißen Ratte und die dabei auftretenden Veränderungen der Mastzellen und Fettzellen. Beitr. path. Anat. 35, 93–126 (1904).
Numers, C. Von: The role of vitamin C in mucopolysaccharide metabolism of skin. Ann. Med. exp. Fenn. 31, 398–408 (1953).
Padawer, J.: A stain for mast cells and its application in various vertebrates and a mastocytoma. J. Histochem. Cytochem 7, 352–353 (1959).
Percival, G. H., A. M. Drennan, and T. C. Dodds: Atlas of histopathology of the skin. Baltimore: Williams & Wilkins Co. 1947.
Riley, J. F.: Pharmacology and functions of the mast cells. Pharmacol. Rev. 7, 267–277 (1955).
—: The mast cells. Edinburgh: E. S. Livingstone, Ltd. 1959.
- Histamine and heparin in mast cells: why both ? Lancet 1962 II, 40–41.
—: Functional significance of histamine and heparin in tissue mast cells. Ann. N. Y. Acad. Sci. 103, 151–161 (1963).
—, and G. B. West: Tissue mast cells. Studies with a histamine liberator of low toxicity (compound 48/80). J. Path. Bact. 69, 269–282 (1955).
Sandberg, N.: Time relationship between administration of cortisone and wound healing in rats. Acta chir. scand. (in press).
-, and C. Steinhardt: On the effect of cortisone on the histamine formation of skin wounds in the rat. Acta chir. scand. (in press).
Schayer, R. N.: Histamine (G. E. W. Wolstenholm and C. M. O'Connor, ed.), p. 183–188 Boston: Little, Brown & Co. 1956.
Simpson, W. L.: Distribution of mast cells as a function of age and exposure to carcinogenic agents. Ann. N. Y. Acad. Sci. 103, 4–18 (1963).
Wichmann, B. E.: The mast cell count during the process of wound healing. (Experimental investigation in rats.) Acta path. microbiol. scand., Suppl. 108, 7–32 (1955).
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Supported by American Cancer Society Grant No IN-60.
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Miller, L., Whitting, H.W. Mast cells and wound healing of the skin in the rat. Zeitschrift für Zellforschung 65, 597–606 (1965). https://doi.org/10.1007/BF00337070
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DOI: https://doi.org/10.1007/BF00337070