Abstract
We have cloned and sequenced an 8.9-kb mitochondrial-DNA fragment from rapeseed (Brassica napus L.). The nucleotide sequence indicates a gene cluster that encodes four ribosomal proteins (S3, L16, L5, S14), two tRNA genes (trnD, trnK), and the 5′ region of the cob gene. The arrangement of these seven genes is trnD-trnK-rps3-rpl16-rpl5-rps14-cob. The rps3 and rpl16 frames overlap by 131 bp. The rpl5 and rps14 genes are separated by a 4-bp spacer. A 1474-basepair intron is located in the rps3 gene. The tRNAAsp gene (trnD) is very similar to the corresponding gene from chloroplasts (cp-like-tRNAAsp). Gene-specific probes for each ribosomal protein gene, and for the cp-like-trnD, trnK and cob genes, hybridized to a common pre-mRNA of an estimated size of 10 kilobases, indicating that these seven genes may be expressed as a single transcription unit. The rps3-rpl16-rpl5-rps14 region of B. napus mtDNA may function as a ribosomal operon, similar to the S10 and SPC operons of Escherichia coli and to the ribosomal protein operon of the chloroplast genome from Euglena gracilis.
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Anderson S, Bankier AT, Barrell BG, De Bruijn MH, Coulson AR, Drouin J, Eperon IC, Nierlich DP, Roe BA, Sanger F, Schreier PH, Smith AJH, Staden R, Young IG (1981) Nature 290:457–465
Binder S, Sehuster W, Grienenberger JM, Weil JH, Brennicke A (1990) Curr Genet 17:353–358
Bland MM, Livings CS III, Matzinger DF (1986) Mol Gen Genet 204:8–16
Bonen L (1987) Nucleic Acids Res 15:10393–10404
Butow RA, Perman PS, Grossman LI (1985) Science 28:1496–1501
Cerretti DP, Dean D, Davis GR, Bedwell DM, Nomura M (1983) Nucleic Acids Res 11:2599–2616
Christopher Da, Hallick RB (1990) Plant Cell 2:659–671
Conklin PL, Hanson MR (1991) Nucleic Acids Res 19:2701–2705
Erickson L, Beversdorf WD, Pauls KP (1985) Curr Genet 9:679–682
Gualberto JM, Wintz H, Weil JH, Grienenberger JM (1988) Mol Gen Genet 215:118–127
Hiratsuka J, Shimada H, Whittier R, Ishibashi T, Sakamoto M, Mori M, Kondo C, Hnji Y, Sun CR, Meng BY, Li YO, Kanno A, Nishizawa Y, Hirai A, Shinozaki K, Sugiura M (1989) Mol Gen Genet 217:185–194
Hunt MD, Newton KL (1991) EMBO J 10:1045–1052
Joyce PBM, Gray MW (1989a) Nucleic Acids Res 17:5461–5476
Joyce PBM, Gray MW (1989b) Biochim Biophys Acta 1008:355–256
Joyce PBM, Spencer DF, Bonen L, Gray MW (1988) Plant Mol Biol 10:251–262
Lonsdale DM (1989) The plant mitochondrial genome. In: Stump PK, Conn EE (eds) The biochemistry of plants: a comprehensive treatise vol 15. Academic Press, New York, pp 229–295
Makaroff CA, Palmer JD (1987) Nucleic Acids Res 15:5141–5156
Marechal-Drouard L, Weil JH, Guillemaut P (1988) Nucleic Acids Res 16:4777–4788
Marechal-Drouard L, Guillemaut P, Cosset A, Arbogast M, Weber F, Weil JH, Dietrich A (1990) Nucleic Acids Res 18:3689–3696
Michel F, Dujon B (1983) EMBO J 2:33–38
Michel F, Umesono K, Ozeki H (1989) Gene 82:5–30
Newton KJ (1988) Annu Rev Plant Physiol Plant Mol Biol 39:503–532
Oda K, Yamato K, Ohta E, Nakamura Y, Takemura M, Nozato N, Akashi K, Kanegae T, Ogura Y, Kohchi T, Ohyama K (1992) J Mol Biol 223:1–7
Ohyama K, Fukuzawa H, Kochi T, Shira H, Sano T, Umesono K, Shiki Y, Takeuchi M, Chang Z, Aota S, Inokuchi H, Ozeki H (1986) Nature 322:572–574
Post LE, Nomura M (1980) J Biol Chem 255:4660–4666
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual 2nd Edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Sangare A, Lonsdale D, Weil JH, Grienenberger JM (1989) Curr Genet 16:195–201
Sanger F, Nicklen S, Coulson AR (1977) Proc Natl Acad Sci USA 74:5463–5467
Schuster W, Brennicke A (1987) EMBO J 6:2857–2863
Schuster W, Brennicke A (1991) Nucleic Acids Res 19:6923–6928
Schuster W, Unseld M, Wissinger B, Brennicke A (1990a) Nucleic Acids Res 18:229–233
Schuster W, Wissinger B, Unseld M, Brennicke A (1990b) EMBO J 9:263–269
Shinozaki K, Ohme M, Tanaka M, Wakasugi T, Hayashida N, Matsubayashi T, Zaita N, Chunwongse J, Obokata J, Yamaguchi-Shinozaki K, Ohto C, Torazawa K, Meng BY, Sugita M, Deno H, Kamogashira T, Yamada K, Kusuda J, Takaiwa F, Kato A, Tohdoh N, Shimada H, Sugiura M (1986) EMBO J 5:2043–2049
Sprinzl M, Hartmann T, Meissner F, Moll J, Vorderwülbecke T (1987) Nucleic Acids Res 15:53–188
Stern DB, Newton KJ (1986) Methods Enzymol 118:488–496
Suzuki T, Kazama S, Hirai A, Akihama T, Kadowaki K (1991) Curr Genet 20:331–337
Takemura M, Oda K, Yamato K, Ohta E, Nakamura Y, Nozato N, Akashi K, Ohyama K (1992) Nucleic Acids Res 20:3199–3205
Wahleithner JA, Wolstenholme DR (1988) Nucleic Acids Res 16:6897–6913
Wissinger B, Hiesel R, Schuster W, Brennicke A (1988) Mol Gen Genet 212:56–65
Wissinger B, Schuster W, Brennicke A (1990) Mol Gen Genet 224:389–395
Wissinger B, Brennicke A, Schuster W (1992) Trends Genet 8:322–328
Ye F, Albaum M, Markmann-Mulisch U, Abel WO (1993) BioTechniques 14:9–10
Zurawski G, Zurawski SM (1985) Nucleic Acids Res 13:4521–4526
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Ye, F., Bernhardt, J. & Abel, W.O. Genes for ribosomal proteins S3, L16, L5 and S14 are clustered in the mitochondrial genome of Brassica napus L.. Curr Genet 24, 323–329 (1993). https://doi.org/10.1007/BF00336784
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DOI: https://doi.org/10.1007/BF00336784