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Comparison of intermittent injection of nondepolarizing solution with a single flush of UW solution for donor heart preservation

  • Original Articles
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Transplant International

Abstract

Isolated canine hearts were preserved for 6 h at 5°C followed by normothermic reperfusion for 2 h. The dogs were divided into two groups of nine hearts each; group 1 received a nondepolarizing preservation solution in multidose, and group 2 received a single flush of University of Wisconsin (UW) solution. Serum MB-CK and mitochondrial aspartate aminotransferase (m-AAT) concentrations and calcium overload during reperfusion were lower in group 1 than in group 2. At the end of reperfusion, myocardial ATP and total adenine nucleotide concentrations were higher and mitochondrial morphology appeared more intact in group 1 than in group 2. Left ventricular diatolic function was preserved better in group 1 than in group 2. These results suggest that in 6-h heart preservation, a nondepolarizing solution applied in multidose fashion protects the myocardium from the deleterious effects of hypothermia and cardioplegia better than a single flush of UW solution.

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Authors and Affiliations

  1. Department of Thoracic-Cardiovascular Surgery, Tokyo Medical and Dental University, School of Medicine, 1-5-45, Yushima, 113, Bunkyo-ku, Tokyo, Japan

    Hisashi Miyamoto, Makoto Sunamori & Akio Suzuki

Authors
  1. Hisashi Miyamoto
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  2. Makoto Sunamori
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  3. Akio Suzuki
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Miyamoto, H., Sunamori, M. & Suzuki, A. Comparison of intermittent injection of nondepolarizing solution with a single flush of UW solution for donor heart preservation. Transplant Int 6, 63–68 (1993). https://doi.org/10.1007/BF00336646

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  • DOI: https://doi.org/10.1007/BF00336646

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