Summary
Photodynamic inactivation with methylene blue and light (MB+Li) of maximally sensitized phages T4 and T4rII nearly followed single-hit kinetics. Not so mutation induction, which in one phage (T4rII N17) tested showed multi-hit kinetics. This difference and the fact that T4rII phages with a GC base pair at the site of the rII-mutation showed no enhanced backmutation when compared to rII phages with an AT base pair or to “sign” mutants suggests that there is no guanine specificity for MB+Li-induced mutation in phage T4.
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Brendel, M. Induction of mutation in phage T4 by extracellular treatment with methylene blue and visible light. Molec. Gen. Genetics 101, 111–115 (1968). https://doi.org/10.1007/BF00336575
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DOI: https://doi.org/10.1007/BF00336575