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Irradiation of cells in tissue culture

VII. Studies on the susceptibility to bluetongue virus on radiation induced giant cells in vitro

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Summary

The susceptibility of giant cells which had been induced by 1000 to 4000 r of gamma irradiation from a Cobalt60 source was studied in comparison to unirradiated cultures. Three human lines were utilized: a synovial cell (McCoy), an amnion (Fernandes) and a fetal lung (Nakanishi). In the amnion line a complete cytopathogenic effect was observed in the giant cells at the beginning of the third day after inoculation as compared to a similar effect produced on the fifth for the corresponding unirradiated control elements. A similar difference of two days was noted for the synovial cells. The virus titers of the amnion line were similar while the synovial cells produced a difference of approximately one logarithm for the virus obtained from the giant elements. The peak for the viral titer was reached earlier with the use of the irradiated cells.

After treatment with 2000 r of gamma irradiation, the fetal lung strain which had not been found susceptible to bluetongue virus showed a complete cytopathogenic effect four days following virus inoculation.

Giant cells were observed with phase contrast microscopy and following staining in order to study the lesions caused by the virus. Time-lapse phase contrast cinematography was also employed in the study of the giant cell-virus system. No specific lesions were observed.

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Bibliography

  • Auxier, J. A.: A high level Co60 irradiation facility. School of Aviation Medicine, U.S.A.F., Report No 55–40, 1957.

  • Dunnebacke, T. H., and M. B. Reaume: Correlation of the yield of poliovirus with the size of isolated tissue culture cells. Virology 6, 8–13 (1958).

    Google Scholar 

  • Eagle, H.: Propagation in a fluid medium of a human epidermoid carcinoma, strain KB. Proc. Soc. exp. Biol. (N. Y.) 89, 362–364 (1955).

    Google Scholar 

  • Earle, W. R., and F. Highhouse: Culture flasks for use with plane surface substrate tissue cultures. J. nat. Cancer Inst. 14, 841–851 (1954).

    Google Scholar 

  • Fernandes, M. V.: The development of a human amnion strain of cells. Tex. Rep. Biol. Med. 16, 48–58 (1958).

    Google Scholar 

  • : Isolation and propagation of bluetongue virus in tissue culture. Amer. J. vet. Res. 20, 398–408 (1959).

    Google Scholar 

  • Nakanishi, Y. H.: Personal communication 1958.

  • Orr, M. F., M. H. Hardy, and C. M. Pomerat: W. Jacobson'S method for staining whole tissue cultures differentially for Ribo- and Desoxyribonucleoproteins. Anat. Rec. 106, 143–144 (1950).

    Google Scholar 

  • Pomerat, C. M., M. V. Fernandes, Y. H. Nakanishi, and S. P. Kent: Irradiation of Cells in Tissue Culture, V. The effect of gamma irradiation from a Cobalt60 source on human amnion cells in vitro. Z. Zellforsch. 48, 1–9 (1958).

    Google Scholar 

  • Pomerat, C. M., L. Hayflick, M. V. Fernandes, and S. P. Kent: Irradiation of Cells in Tissue Culture. III. Measurement of injury by pH changes and cytopathology in test tube cultures. Z. Zellforsch. 47, 245–250 (1958).

    Google Scholar 

  • Pomerat, C. M., S. P. Kent, and L. C. Logie: Irradiation of Cells in Tissue Culture I. Giant cell induction in strain cultures versus elements from primary expiants. Z. Zellforsch. 47, 158–174 (1957).

    Google Scholar 

  • Puck, T. T.: Quantitative measurement of virus action by cell plating techniques. In: Perspectives in Virology (Morris Pollard, editor). New York: John Wiley & Sons 1959.

    Google Scholar 

  • Reed, L. J., and H. Muench: Simple method of estimating 50 per cent endpoints. Amer. J. Hyg. 27, 493–497 (1938).

    Google Scholar 

  • Rose, G. G.: A separable and multipurpose tissue culture chamber. Tex. Rep. Biol. Med. 12, 1074–1083 (1954).

    Google Scholar 

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Authors and Affiliations

  1. Tissue Culture Laboratory, Department of Anatomy, The University of Texas, Medical Branch, Galveston, Texas

    Mario V. Fernandes

  2. Laboratorio Nacional de Investigação Veteriária, Lisbon, Portugal

    Mario V. Fernandes

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  1. Mario V. Fernandes
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This research was supported by funds provided under Contract AF 41(657)-198 with the School of Aviation Medicine, USAF, Randolph Air Force Base, Texas.

Fellow of the Instituto de Alta Cultura and the Fundaçaõ Calouste Gulbenkian of Lisbon, Portugal. Permanent address: Laboratorio Nacional de Investigação Veterinária, Lisbon, Portugal.

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Fernandes, M.V. Irradiation of cells in tissue culture. Zeitschrift für Zellforschung 50, 433–443 (1959). https://doi.org/10.1007/BF00336567

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  • DOI: https://doi.org/10.1007/BF00336567

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