Summary
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1.
The findings of other investigators with respect to the perineurium being an efficient diffusion barrier are confirmed. This also holds true for a protein of small molecular weight and size.
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2.
By means of endoneurial or epineurial application the substance never reaches the third perineurial cell layer. Pinocytotic vesicles account for the transport of peroxidase through the cytoplasm of the cells.
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3.
The perineural cell membranes and the interconnecting tight junctions form a barrier for horseradish peroxidase, as do the nerve fibers.
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4.
In myelinated fibers the barring site appears to be localized in the lamellae and in the first part of the outer mesaxon. In non-myelinated fibers evidence was found that the axolemma prevents the peroxidase from penetrating into the axon.
Zusammenfassung
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1.
Die bisher erhobenen Befunde über das Perineurium als Diffusionsbarriere werden auch für ein Protein von kleinem Molekulargewicht und geringer Molekülgröße bestätigt.
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2.
Bei epineuraler wie endoneuraler Applikation dringt die Peroxdase nie bis zur 3. Perineuralzellschicht vor. Dabei transportieren Pinozytosevesikel die Substanz durch das Zytoplasma der Zellen.
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3.
Die Diffusion wird durch die Zellmembran der Perineuralzellen und die engen Verbindungen (tight junctions) der Kontaktstellen verhindert.
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4.
Es besteht ferner auch eine Barrierenfunktion gegenüber der Peroxydase an den Nervenfasern: Bei markhaltigen Fasern scheint sie in den Marklamellen und im Beginn des mesaxonalen Spaltes bei den marklosen im Axolemm zu liegen.
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Literatur
Becker, N. H., Almazon, R.: Evidence for the functional polarization of micropinozytotic vesicles in the rat chorioid plexus. J. Histochem. Cytochem. 16, 278–280 (1968).
—, Hirano, A., Zimmermann, H. M.: Observations of the distribution of exogenous peroxidase in the rat cerebrum. J. Neurophath. exp. Neurol. 27, 439–452 (1968).
—, Novikoff, A. B., Zimmermann, H. M.: Fine structure observations of the uptake of intravenously injected peroxidase by the rat chorioid plexus. J. Histochem. Cytochem. 15, 160–165 (1968).
Burkel, W. E.: The histological fine structure of perineurium. Anat. Rec. 158, 177–189 (1967).
Causey, C., Palmer, E.: The epineural sheath of a nerve as a barrier to the diffusion of phosphations. J. Anat. (Lond.) 87, 30–36 (1953).
Crescitelli, F.: Nerve sheath as a barrier to the action of certain substances. Amer. J. Physiol. 166, 229–240 (1951).
Emiroglu, F.: The permeability of the peripheral nerve sheath in frogs. Arch. int. Physiol. 63, 161–180 (1955).
Farquhar, M. G., Palade, G. E.: Cell junctions in amphibian skin. J. Cell Biol. 26, 263 (1965).
Feng, T. P., Gerard, R. W.: Mechanism of nerve asphyxiation: With a note on the nerve sheath as a diffusion barrier. Proc. Soc. exp. Biol. (N. Y.) 27, 1073–1076 (1930).
—, Liu, Y. M.: The connective tissue sheath of the nerve as an effective diffusion barrier. J. cell comp. Pysiol. 34, 1–6 (1949).
Gamble, H. L.: Comparative electron-microscopic observations on the connective tissues of a peripheral nerve and a spinal nerve root in the rat. J. Anat. (Lond.) 98, 17–25 (1964).
Graham, R. C., Karnovsky, M. J.: The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: Ultrastructural cytochemistry by a new technique. J. Histochem. Cytochem. 14, 291–302 (1966).
Haftek, D., Thomas, P. K.: Electron-microscope observations in the effects of localized crush injuries on the connective tissues of peripheral nerve. J. Anat. (Lond.) 103, 233–243 (1968).
Hirano, A., Becker, N. H., Zimmermann, H. M.: Pathological alterations in the cerebral endothelial cell barrier to peroxidase. Arch. Neurol. (Chic.) 20, 300–308 (1969).
Karnovsky, J. M.: A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. J. Cell Biol. 27, 137A (1965).
—: The ultrastructural basis of capillary permeability studied with peroxidase as a tracer. J. Cell Biol. 35, 213–236 (1967).
Key, A., Retzius, G.: Studien in der Anatomie des Nervensystems und des Bindegewebes, Bd. 2, S. 102–112. Stockholm 1876.
Krnjevic, D.: Some observations on perfused frog sciatic nerve. J. Physiol. (Lond.) 123, 338 (1954).
Landon, D. N.: Electron microscopy of muscle spindles. Control and innervation of skeletal muscle. A symposium at Queen's College, Dundee, p. 96–110, Sept. 1965.
Lehmann, H. J.: The epineurium as a diffusion barrier. Nature (Lond.) 172, 1045–1046 (1953).
—: Über Struktur und Funktion der perineuralen Diffusionsbarriere. Z. Zellforsch. 46, 232–241 (1957).
Lorente de Nó, R.: The ineffectiveness of the connective tissue sheath of nerve as a diffusion barrier. J. cell. comp. Physiol. 35, 195–240 (1950).
Malaty, H. A., Bourne, G. H.: Histochemistry of succinic dehydrogenase. Nature (Lond.) 171, 295–297 (1953).
Matin, K. H.: Untersuchungen über die perineurale Diffusionsbarriere am gefriergetrockneten Nerven. Z. Zellforsch. 64, 404–428 (1964).
McCabe, J. S., Low, F. N.: The subarachnoid angle: An area of transition in peripheral nerve. Anat. Rec. 164, 15–33 (1969).
O'Daly, Imaeda, T.: Electron microscopic study of Wallerian degeneration in cutaneous nerves caused by mechanical injury. Lab. Invest. 17, 744–766 (1967).
Reese, T. S., Karnovsky M.: Fine structural localization of a blood-brain barrier to exogenous peroxidase. J. Cell Biol. 34, 207–217 (1967).
Reiss, J.: Prinzipien der histochemischen Lokalisation von Cytochromoxydase. Mikroskopie 22, 1–10 (1967).
Robin, C.: Mémoire sur le périnèvre, espèce nouvelle d'élément anatomique qui entre dans la composition du tissues des nerfs. C. R. Soc Biol. (Paris), Ie sér. 1, 99 (1854).
Röhlich, P., Weiss, M.: Studies on the histology and permeability of the peripheral nervous barrier. Acta morph. (Basel) 5, 335–347 (1955).
Shanta, T. R., Bourne, G. H.: The perineural epithelium: A metabolically active, continuous protoplasmic cell barrier surrounding peripheral nerve fasciculi. J. Anat. (Lond.) 96, 527–537 (1962).
—: The perineural epithelium: Nature and significance. Nature (Lond.) 199, 577–579 (1963).
—: The perineural epithelium — A new concept: In: The structure and function (G. H. Bourne, ed.) vol. 1, Struct. I, p. 379–459. New York and London: Academic Press 1968.
Straus, W.: Segregation of an intravenously injected protein by “droplets” of the cells of rat kidney. J. biophys. biochem. Cytol. 3, 1037 (1957).
—: Colorimetric analysis with N, N-Dimethyl-p-phenylenediamine of the uptake of intravenously injected horseradish peroxidase by various tissues of the rat. J. Cytol. 4, 541–550 (1958).
Sunderland, S: The connective tissues of peripheral nerves. Brain 88, 841–854 (1965).
—, Bradley, K. C.: The perineurium of peripheral nerves. Anat Rec. 113, 125–142 (1952).
Thomas, E.: Die genaue Lokalisation von Dehydrogenasen im Nervensystem. IV. internationaler Kongreß für Neuropathologie vom 4.-8. Sept. 1961. München. H. Jacob Editor. Vol. 1, p. 102–104. Stuttgart: Thieme 1962.
—: Histochemie and Histopathochemie des peripheren Nervensystems bei Verletzungen und Tumoren. Veröffentlichungen aus der morphologischen Pathologie, Heft 18, Stuttgart: Gustav Fischer 1968.
—, Pearse, A. G. E.: The fine localization of dehydrogenases in the nerves system. Histochemie 2, 266–282 (1961).
Thomas, P. K.: The connective tissue of peripheral nerve. An electron microscopic study. J. Anat. 97, 35–44 (1963).
—, Jones, D. G.: The cellular response to nerve injury 2. Regeneration of the perineurium after nerve section. J. Anat. 101, 45–55 (1967).
Venable, J. H., Coggeshall, R.: A simplified lead citrate stain for use in electron microscopy. J. Cell biol. 25, 407–408 (1965).
Waggener, J. D., Bunn, S. M., Beggs, J.: The diffusion of ferritin within peripheral nerve sheath: An electron microscopy study. J. Neuropath. 24, 430–443 (1965).
Zacks, S.: Uptake of exogenous horseradish peroxidase by coated vesicles in mouse neuromuscular junctions. J. Histochem. Cytochem. 17, 161–170 (1969).
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Die elektronenmikroskopischen Untersuchungen wurden im elektronenmikroskopischen Laboratorium bei Herrn Dr. Schröder durchgeführt, dem ich die Anregung zur Verwendung der Peroxydase verdanke. Den techn. Assistentinnen Frl. Frenk, Frau Schilling und Frl. Eckstein danke ich für ihre technische Unterstützung.
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Flemm, H. Das Perineurium als Diffusionsbarriere gegenüber Peroxydase bei epi- und endoneuraler Applikation. Z. Zellforsch. 108, 431–445 (1970). https://doi.org/10.1007/BF00336529
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DOI: https://doi.org/10.1007/BF00336529