Summary
The effect of 0,4 μg of actinomycin per ml medium on DNA synthesis in synchronous cultures of Tetrahymena pyriformis strain HSM was studied. Synchronous cultures were obtained by selecting cells from a stock culture which were all in the same division phase In this concentration actinomycin inhibits cell division but permits the normal doubling of DNA and furthermore another period of macronuclear DNA synthesis. In this additional DNA production phase the rate and the synchrony of DNA synthesis is reduced as revealed by autoradiography. The production of additional DNA was demonstrated by photometric determination of Feulgen stainable material. These findings indicate that the onset of DNA synthesis is independent of a preceding cell division, of a preceding nuclear division, of the average amount of DNA present, and of the main portion of RNA synthesis.
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Literatur
Alfert, M.: A cytochemical study of oogenesis and cleavage in the mouse. J. cell. comp. Physiol. 36, 381–410 (1950).
Cameron, I. L., and G. E. Stone: Relation between the amount of DNA per cell and the duration of DNA synthesis in three strains of Tetrahymena pyriformis. Exp. Cell Res. 36, 510–514 (1964).
Cleffmann, G.: Die Schwellen der Hemmung der Nucleinsäuresynthese und der Zellteilung durch Actinomycin bei Tetrahymena pyriformis. Z. Zellforsch. 67, 343–350 (1965).
Gall, J. G.: Macronuclear duplication in the ciliated protozoan Euplotes. J. biophys. biochem. Cytol. 5, 295–308 (1959).
Kimball, R. F., and T. Barka: Quantitative cytochemical studies on Paramaecium aurelia. II. Feulgen microspectro-photometry of the macronucleus during exponential growth. Exp. Cell Res. 17, 173–182 (1959).
Lang, W., u. W. Maurer: Zur Verwendbarkeit von feulgengefärbten Schnitten für quantitative Autoradiographie mit markiertem Thymidin. Exp. Cell Res. 39, 1–9 (1965).
Maruyama, Y., and K. G. Lark: Periodic synthesis of bacterial nucleic acids in the absence of protein synthesis. Exp. Cell Res. 25, 161–169 (1961).
Maurer, W., u. E. Primbsch: Größe der Selbstabsorption bei der H3-Autoradiographie. Exp. Cell Res. 33, 8–18 (1964).
McDonald, B. B.: Quantitative aspects of deoxyribose nucleic acid (DNA) metabolism in an amicronucleate strain of Tetrahymena. Biol. Bull. 114, 71–84 (1958).
Mita, T.: Effects of actinomycin D on the RNA synthesis and synchronous cell division of Tetrahymena GL. Biochem biophys. Acta (Amst.) 103, 182–185 (1965).
Nygaard, O. F., and S. Güttes: Effects of ionizing radiations on a slime mould with synchronous mitosis. Int. J. Rad. Biol. 5, 33–44 (1962).
Okazaki, T., and R. Okazaki: Studies of deoxyribonucleic acid synthesis and cell growth in the deoxyribaside requiring bacteria Lactobacillus acidophilus. II. Deoxyribonucleic acid synthesis in relation to ribonucleic acid and protein synthesis. Biochem. biophys. Acta (Amst.) 35, 434–445 (1959).
Prescott, D. M.: Variations in the individual generation times of Tetrahymena geleii HS. Exp. Cell Res. 16, 279–284 (1959).
—: Autoradiography with liquid emulsion. In: D. M. Prescott, Methods in cell physiology, p. 365–370. New York: Academic Press 1964.
Ruthmann, A.: Autoradiographische und mikrophotometrische Untersuchungen zur DNS-Synthese von Bursaria truncatella. Arch. Protistenk. 107, 117–130 (1964).
Scherbaum, O.: The content and composition of nucleic acids in normal and synchronously dividing cultures of Tetrahymena pyriformis. Exp. Cell Res. 13, 24–30 (1957).
Shepard, D. C.: Production and limitation of excess DNA in ultraviolettirradiated Tetrahymena. J. Cell Biol. 23, 86A (1964).
Stone, G. E., and I. L. Cameron: Methods for using Tetrahymena in studies of the normal cell cycle. In: D. M. Prescott, Methods in cell physiology, p. 127–141. New York: Academic Press 1964.
Swift, H.: The constancy of desoxyribose nucleic acid in plant nuclei. Proc. nat. Acad. Sci. (Wash.) 36, 643–654 (1950).
Vendrely, R., et C. V. Vendrely: La teneur du noyau cellulaire en acide desoxyribonucléique à travers les organes, les individues et les espèces animales. Experientia (Basel) 4, 269 (1948).
Zeuthen, E.: Independent cycles of cell division and of DNA synthesis in Tetrahymena. In: R. J. C. Harris, Cell growth and cell division, p. 1–7. New York: Academic Press 1963.
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Herrn Professor Dr. R. Danneel zu seinem 65. Geburtstag.
Unterstützt durch Sachbeihilfen der Deutschen Forschungsgemeinschaft.
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Cleffmann, G. Bildung Zusätzlicher DNS nach Blockierung der Zellteilung von Tetrahymena durch Actinomycin. Zeitschrift für Zellforschung 70, 290–297 (1966). https://doi.org/10.1007/BF00336497
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DOI: https://doi.org/10.1007/BF00336497