Summary
We have sequenced the intron in the large subunit ribosomal RNA gene from the mitochondrion of Kluyveromyces lactis. It is a typical group I intron but, unlike the corresponding intron (r1) in Saccharomyces cerevisiae, it does not contain an open reading frame. This intron is widespread in the genus Kluyveromyces although intron-less strains were also found in some species of this genus. Sequences homologous to the open reading frame of the S. cerevisiae ribosomal intron were detected in some strains of K. waltii, K thermotolerans and K. africanus.
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References
Augustin S, Muller MW, Schweyen RJ (1990) Nature 343:383–386
Bos JL, Osinga KA, Van der Horst G, Hecht NB, Tabak HF, Van Ommen GJ, Borst P (1980) Cell 20:207–214
Brunner A, Coria R (1989) Yeast 5:209–218
Burke JM, Belfort M, Cech TR, Davies RW, Schweyen RJ, Shub DA, Szostak JW, Tabak HF (1987) Nucleic Acids Res 15:7217–7221
Delahodde A, Goguel V, Becam AM, Creusot F, Perea J, Banroques J, Jacq C (1989) Cell 56:431–441
De Zamaroczy M, Bernardi G (1986) Gene 47:155–177
Dujon B (1981) In Strathern JN, Jones EW, Broach JR (eds) The molecular biology of the yeast Saccharomyces. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp 505–635
Dujon B, Colleaux L, Jaquier A, Michel F, Monteilhet C (1986) In Wickner RB, Hinnebusch A, Lambowitz AM, Gunsalus IC, Haender A (eds) Extrachromosomal elements in lower eukaryotes. Plenum Press, New York, pp 5–27
Faye G, Kujawa C, Fukuhara H (1974) J Mol Biol 88:185–203
Garriga G, Lambowitz AM (1983) J Biol Chem 258:14745–14748
Green NR, Grimm MF, Goewert RR, Collins RA, Cole MD, Lambowitz AM, Heckman JE, Yin S, RajBandary UL (1981) J Biol Chem 256:2027–2034
Jacquier A, Dujon B (1983) Mol Gen Genet 192:487–499
Jacquier A, Dujon B (1985) Cell 41:383–394
Lambowitz AM (1989) Cell 56:323–326
Limieux B, Turmel M, Lemieuc C (1988) Mol Gen Genet 212:48–55
Locker J (1979) Anal Biochem 98:358–367
Macreadie IG, Scott RM, Zinn AR, Butow RA (1985) Cell 42:395–402
Mota EM, Collins RA (1988) Nature 332:654–656
Muscarella DE, Vogt VM (1989) Cell 56:443–454
Netzker R, Kochel HG, Basak N, Kuntzel H (1982) Nucleic Acids Res 10:4783–4801
Perlman PS, Butow RA (1989) Science 246:1106–1109
Quirk SM, Bell-Pedersen D, Belfort M (1989) Cell 56:455–465
Ragnini A, Fukuhara H (1988) Nucleic Acids Res 16:8433–8442
Ragnini A, Grisanti P, Rinaldi T, Frontali L, Palleschi C (1991) Curr Genet 19:169–174
Sharp PA (1985) Cell 42:397–400
Trinkl H, Wolf K (1986) Gene 45:289–297
Van der Walt JP, Johannsen E (1984) In Kreger van Rij NJW (ed) The yeast, a taxonomic study. 3rd ed. Elsevier, Amsterdam. pp. 224–251
Woodson SA, Cech TR (1989) Cell 57:335–345
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Communicated by K. Wolf
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Wilson, C., Fukuhara, H. Distribution of mitochondrial r1-type introns and the associated open reading frame in the yeast genus Kluyveromyces . Curr Genet 19, 163–167 (1991). https://doi.org/10.1007/BF00336482
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DOI: https://doi.org/10.1007/BF00336482