Summary
The diversity of Bradyrhizobium japonicum in agricultural fields has not been well characterized. Therefore a study was conducted to determine the serotypic diversity of B. japonicum both within and among six fields in the Coastal Plain and Piedmont of North Carolina where soybeans [Glycine max (L.) Merr.] are grown. Nodule samples were collected from non-inoculated standing soybean crops. Both nodules and isolates were typed by the enzyme-linked immunosorbent assay (ELISA) technique. Serotypes and their proportions varied both within and among locations. Common serotypes in order of abundance across all sites were 76, M1 (multiple reaction beyween 31 and 94), 94, 24, and 122, and together accounted for over 66% of the typable reactions. No cultivar effect on serotype distribution was observed. Unknown types ranged from 4 to 24%. Based on the total number of serotypes identified and the Shannon diversity index (H′), the mean population diversity was 0.76 for the Piedmont sites and 0.91 for the Coastal Plain sites.
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Paper no. 12315 of the Journal Series of the North Carolina Agricultural Research Service, Raleigh, NC 27695-7643. The use of trade names in this publication does not imply endorsement by the North Carolina Agricultural Research Service of the products named or criticism of similar ones not mentioned
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Mpepereki, S., Wollum, A.G. Diversity of indigenous Bradyrhizobium japonicum in North Carolina soils. Biol Fertil Soils 11, 121–127 (1991). https://doi.org/10.1007/BF00336376
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DOI: https://doi.org/10.1007/BF00336376