Abstract
The effect of Actinomycin-D was studied on chiasma frequency in Vicia faba (var. Weibull's Akerabona). A significant decrease in chiasma frequency over control was observed in all cases irrespective of the time of fixation and chemical concentration. However, the maximum decrease was found in the material fixed after a recovery period of 24 hours. The decrease in chiasma frequency showed a linear relationship with time of recovery. Besides reduction in the chiasma frequency, cells showing complete failure of homologous pairing were also observed. These findings are discussed in the light of actinomycin-D action.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Beadle, G. W.: Further studies in asynaptic maize. Cytologia (Tokyo) 4, 269–287 (1933).
Darlington, C. D.: A cytological demonstration of “genetic crossing over”. Proc. roy. Soc. B 107, 50–59 (1930).
Goldberg, I. H., M. Rabinowitz, and E. Reich: Basis of Actinomycin action. I. DNA binding and inhibition of RNA polymerase synthetic reactions by Actinomycin. Proc. nat. Acad. Sci. (Wash.) 48, 2094 (1962).
Howard-Flanders, P., and R. P. Boyce: A biochemical pathway in the repair after UV irradiation that may have steps in common with those of genetic recombination by breakage and reunion (Abstr.). Genetics 50, 256–257 (1964).
Jain, H. K., and U. Singh: Actinomycin-D induced chromosome breakage and suppression of meiosis in the locust Schistocerea gregaria. Chromosoma (Berl.) 21, 463–471 (1967).
Janssens, F. A.: La chiasmatypie dans les insects. Cellule 34, 135–359 (1924).
Kaufman, B. P., H. Gay, and M. I. McElderry: Effect of ribonuclease on crossing over. Proc. nat. Acad. Sci. (Wash.) 43, 255–261 (1957).
Kirk, J. M.: The mode of action of actinomycin-D. Biochim. biophys. Acta (Amst.) 42, 167–169 (1960).
Lawrence, C. W., and D. R. Davis: The mechanism of recombination in Chlamydomonas reinhardi. I. The influence of inhibitors of protein synthesis on intergenic recombination. Mutation Res. 4, 137–146 (1967).
Meselson, M.: On the mechanism of genetic recombination between DNA molecules. J. molec. Biol. 9, 734–745 (1964).
Rees, H.: Genotypic control of chromosome behaviour in rye. I. Inbred lines. Heredity 9, 93–116 (1955).
Sears, E. R., and M. Okamoto: Intergenomic chromosome relationships of non-homologous chromosomes in wheat. Proc. 10th Int. Congr. Genet. 2, 258–259 (1958).
Smith, B. R.: Genetic control of recombination. I. The recombination of 2 gene in Neurospora crassa.. Heredity 21, 481–498 (1966).
Suzuki, D. T.: Effects of actinomycin-D on crossing over in Drosophila melanogaster. Genetics 51, 11–21 (1965).
Sybenga, J.: The zygomere as hypothetical unit of chromosome pairing initiation. Genetica (s'Gravenhage) 37, 186–198 (1966).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sharma, R.P., Singh, D. The effect of Actinomycin-D on chiasma frequency in Vicia faba . Chromosoma 24, 309–313 (1968). https://doi.org/10.1007/BF00336198
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00336198