Summary
Data on the use of a biological containment system in microcosms are presented. The system is based on a transposon Tn5-cassette containing a fusion of the lac promoter and gef controlled by lacIQ. The suicide function was inserted into different bacterial hosts and its induction by isopropyl-β-d-thiogalactoside (IPTG) was tested in sterile and non-sterile soil microcosms. The results suggest that the killing effect of this gef-based suicide mechanism is insufficient under soil conditions.
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Henschke, R.B., Henschke, E.J. & Schmidt, F.R.J. Use of the gef gene in a biological containment system in soil microcosms. Biol Fertil Soils 11, 301–305 (1991). https://doi.org/10.1007/BF00335852
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DOI: https://doi.org/10.1007/BF00335852