Summary
NO and N2O release rates were measured in an acidic forest soil (pH 4.0) and a slightly alkaline agricultural soil (pH 7.8), which were incubated at different O2 concentrations (<0.01 – 20% O2) and at different NO concentrations (40 – 1000 ppbv NO). The system allowed the determination of simultaneously operating NO production rates and NO uptake rate constants, and the calculation of a NO compensation concentration. Both NO production and NO consumption decreased with increasing O2. NO consumption decreased to a smaller extent than NO production, so that the NO compensation concentrations also decreased. However, the NO compensation concentrations were not low enough for the soils to become a net sink for atmospheric NO. The release of N2O increased relative to NO release when the gases were allowed to accumulate instead of being flushed out. The forest soil contained only denitrifying, but not nitrifying bacteria, whereas the agricultural soil contained both. Nevertheless, NO release rates were less sensitive to O2 in the forest soil compared to the agricultural soil.
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References
Abou Seada MNI, Ottow JCG (1985) Effect of increasing oxygen concentration on total denitrification and nitrous oxide release from soil by different bacteria. Biol Fertil Soils 1:31–38
American Public Health Association (1969) Standard methods for examination of water and waste water including bottom sediments and sludge. Am Public Health Assoc, Washington DC, pp 604–609
Bazylinski DA, Blakemore RP (1983) Denitrification and assimilatory nitrate reduction in Aquaspirillum magnetotacticum. Appl Environ Microbiol 46:1118–1124
Betlach MR, Tiedje JM (1981) Kinetic explanation for accumulation of nitrite, nitric oxide, and nitrous oxide during bacterial denitrification. Appl Environ Microbiol 42:1074–1084
Cho CM, Mills JG (1979) Kinetic formulation of the denitrification process in soil. Can J Soil Sci 59:249–257
Conrad R (1991) Flux of NOx between soil and atmosphere: Importance and soil microbial metabolism. In: Soerensen J, Revsbech NP (eds) Denitrification in soil and sediment. Plenum, New York (in press)
Conrad R, Seiler W (1980) Field measurements of the loss of fertilizer nitrogen into the atmosphere as nitrous oxide. Atmos Environ 14:555–558
Crutzen PJ (1979) The role of NO and NO2 in the chemistry of the troposphere and stratosphere. Annu Rev Earth Planet Sci 7:443–472
Firestone MK, Davidson EA (1989) Microbiological basis of NO and N2O production and consumption. In: Andreae MO, Schimel DS (eds) Exchange of trace gases between terrestrial ecosystems and the atmosphere. Dahlem Konferenzen. Wiley, Chichester, pp 7–21
Focht DD, Verstraete W (1977) Biochemical ecology of nitrification and denitrification. Adv Microb Ecol 1:135–214
Gök M, Ottow JCG (1988) Effect of cellulose and straw incorporation in soil on total denitrification and nitrogen immobilization at initially aerobic and permanent anaerobic conditions. Biol Fertil Soils 5:317–322
Johansson C, Galbally IE (1984) Production of nitric oxide in loam under aerobic and anearobic conditions. Appl Environ Microbiol 47:1284–1289
Kroeckel L, Stolp H (1985) Influence of oxygen on denitrification and aerobic respiration in soil. Biol Fertil Soils 1:189–193
Kuenen JG, Robertson LA (1988) Ecology of nitrification and denitrification. In: Cole JA, Ferguson S (eds) The nitrogen and sulphur cycles. Cambridge University Press, Cambridge, pp 161–218
Nägele W, Conrad R (1990) Influence of soil pH on the denitrifier populations and their potential to reduce nitrate to NO and N2O. FEMS Microbiol Ecol 74:49–57
Ottow JCG, Fabig W (1985) Influence of oxygen aeration on denitrification and redox level in different bacterial batch cultures. In: Caldwell DE, Brierley JA, Brierley CL (eds) Planetary ecology. Van Nostrand Reinhold, New York, pp 427–440
Parkin TB (1987) Soil microsites as a source of denitrification variability. Soil Sci Soc Am J 51:1194–1199
Parkin TB, Tiedje JM (1984) Application of a soil core method to investigate the effect of oxygen concentration on denitrification. Soil Biol Biochem 16:331–334
Pfennig N (1978) Rhodocyclus purpureus gen nov, and sp nov, a ringshaped vitamin B12-requiring member of the family Rhodospirillaceae. Int J Syst Bact 28:283–288
Remde A, Conrad R (1991a) Role of nitrification and denitrification for NO metabolism in soil. Biogeochem (in press)
Remde A, Conrad R (1991b) Metabolism of nitric oxide in soil and denitrifying bacteria. FEMS Microbiol Ecol (in press)
Remde A, Slemr F, Conrad R (1989) Microbial production and uptake of nitric oxide in soil. FEMS Microbiol Ecol 62:221–230
Robertson LA, Kuenen JG (1984) Aerobic denitrification: A controversy revised. Arch Microbiol 139:341–354
Schlichting E, Blume HP (1966) Bodenkundliches Praktikum. Paul Parey, Hamburg
Sexstone AJ, Revsbech NP, Parkin TB, Tiedje JM (1985) Direct measurement of oxygen profiles and denitrification rates in soil aggregates. Soil Sci Soc Am J 49:645–651
Singh HB (1987) Reactive nitrogen in the troposphere: Chemistry and transport of NOx and PAN. Env Sci Technol 21:320–327
Slemr F, Seiler W (1984) Field measurements of NO and NO2 emissions from fertilized and unfertilized soils. J Atmos Chem 2:1–24
Tiedje JM (1988) Ecology of denitrification and dissimilatory nitrate reduction to ammonium. In: Zehnder AJB (ed) Biology of anaerobic microorganisms. Wiley, New York, pp 179–244
Widdel F, Kohring GW, Mayer F (1983) Studies on dissimilatory sulfatereducing bacteria that decompose fatty acids. 3. Characterization of the filamentous gliding Desulfonema limicola gen nov, sp nov, and Desulfonema magnum sp nov. Arch Microbiol 134:286–294
Zafiriou OC, Hanley QS, Snyder G (1989) Nitric oxide and nitrous oxide production and cycling during dissimilatory nitrite reduction by Pseudomonas perfectomarina. J Biol Chem 264:5694–5699
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Krämer, M., Conrad, R. Influence of oxygen on production and consumption of nitric oxide in soil. Biol Fertil Soils 11, 38–42 (1991). https://doi.org/10.1007/BF00335832
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DOI: https://doi.org/10.1007/BF00335832