Skip to main content
SpringerLink
Log in

Osmium tetroxide versus glutaraldehyde fixation in adrenomedullary tissue

  • Published:
Zeitschrift für Zellforschung und Mikroskopische Anatomie Aims and scope Submit manuscript

Summary

Osmium tetroxide and glutaraldehyde fixation of adrenomedullary tissue presents evidence that these two fixatives preserve the tissue in quite different manners. Not only is the type of fixative of importance, but also the osmolarity of the fixatives is a prime factor in producing an accurate pictorial account of catecholamines.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bargmann, W., u. E. Lindner: Über den Feinbau des Nebennierenmarkes des Igels (Erinaceus europaeus L.). Z. Zellforsch. 64, 868–912 (1964).

    Google Scholar 

  • Benedeczky, I., A. Puppi, and A. Tigyi: Histochemical and electron microscopical study of the adrenal medulla of the grass snake (Natrix natrix). Acta biol. Acad. Sci. hung. 15, 271–284 (1965).

    Google Scholar 

  • Bennett, H. S., and J. H. Luft: S-collidine as a basis for buffering fixatives. J. biophys. biochem. Cytol. 6, 113–114 (1959).

    Google Scholar 

  • Callas, G., and J. G. Wood: Light and electron microscopic observations on mouse adrenomedullary tissue following stress and drug administration. Anat. Rec. 151, 331 (1965).

    Google Scholar 

  • Caulfield, J. B.: Effects of varying the vehicle for OsO4 in tissue fixation. J. biophys. biochem. Cytol. 3, 827–830 (1957).

    Google Scholar 

  • Coupland, R. E.: Electron microscopic observations of the rat adrenal medulla. I. The ultrastructure and organization of chromaffin cells in the normal adrenal medulla. J. Anat. (Lond.) 99, 231–254 (1965).

    Google Scholar 

  • —, A. S. Pyper, and D. Hopwood: A method for differentiating between noradrenaline and adrenaline-storing cells in the light and electron microscopes. Nature (Lond.) 201 1240–1242 (1964).

    Google Scholar 

  • Eränkö, O., and L. Hänninen: Electron microscopic observations on the adrenal medulla of the rat. Acta path. microbiol. scand. 50, 126–132 (1960).

    Google Scholar 

  • Hillarp, N. A.: Isolation and some biochemical properties of the catecholamine granules in the cow adrenal medulla. Acta physiol. scand. 43, 82–96 (1958).

    Google Scholar 

  • Lever, J. D.: Electron microscopic observations on the normal and denervated adrenal medulla of the rat. Endocrinology 57, 621–635 (1955).

    Google Scholar 

  • Millonig, G.: Advantages of a phosphate buffer for OsO4 solution in fixation. J. appl. Phys. 32, 1637 1961).

    Google Scholar 

  • Reynolds, E. S.: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17, 208–212 (1963).

    Google Scholar 

  • Sjöstrand, F. S., u. R. Wetzstein: Elektronenmikroskopische Untersuchung der phaeochromen (chromaffinen) Granula in den Markzellen der Nebenniere. Experentia (Basel) 12, 196–199 (1955).

    Google Scholar 

  • Torack, R. M.: The extracellular space of rat brain following perfusion fixation with glutaraldehyde and hydroxyadipaldehyde. Z. Zellforsch. 66, 352–364 (1965).

    Google Scholar 

  • Tormey, J. McD.: Differences in membrane configuration between osmium tetroxide-fixed and glutaraldehyde-fixed ciliary epithelium. J. Cell Biol. 23, 658–664 (1964).

    Google Scholar 

  • Tramezzani, J. H., S. Chiocchio, and G. F. Wassermann: A technique for light and electron microscopic identification of adrenalin- and noradrenalin-storing cells. J. Histochem. Cytochem. 12, 890–899 (1964).

    Google Scholar 

  • Wassermann, G., and J. H. Tramezzani: Separate distribution of adrenaline- and noradrenaline-secreting cells in the adrenal of snakes. Gen. comp. Endocr. 3, 480–489 (1963).

    Google Scholar 

  • Wetzstein, R.: Elektronenmikroskopische Untersuchungen am Nebennierenmark von Maus, Meerschweinchen und Katze. Z. Zellforsch. 46, 517–576 (1957).

    Google Scholar 

  • Wood, J. G., and R. J. Barrnett: Histochemical differentiation of epinephrine and norepinephrine granules in the adrenal medulla with the electron microscope. Anat. Rec. 145, 301–302 (1963).

    Google Scholar 

  • —: Histochemical demonstration of norepinephrine at a fine structural level. J. Histochem. Cytochem. 12, 197–209 (1964).

    Google Scholar 

  • Yates, R. D., J. G. Wood, and D. Duncan: Phase and electron microscopic observations on two cell types in the adrenal medulla of the Syrian Hamster. Tex. Rep. Biol. Med. 20, 494–502 (1962).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departments of Anatomy, The University of Arkansas Medical Center, 72201, Little Rock, Arkansas

    Joe G. Wood & Gerald Callas

  2. The University of Texas Medical Branch, 77551, Galveston, Texas

    Joe G. Wood & Gerald Callas

Authors
  1. Joe G. Wood
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Gerald Callas
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Supported by United States Public Health Service Grants 5-Tl-GM-459, NB 05093-02, FR 0505-01, NB 00690-11 and National Science Foundation Grant GB 25 96.— With the technical assistance of John Yates, Earl Pitsinger and Brenda Ryker.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wood, J.G., Callas, G. Osmium tetroxide versus glutaraldehyde fixation in adrenomedullary tissue. Zeitschrift für Zellforschung 71, 261–270 (1966). https://doi.org/10.1007/BF00335751

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00335751

Keywords

Search

Navigation