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Aspects ultrastructuraux de la glande mammaire de lapine pendant la lactogénèse

Ultrastructural aspects of the rabbit mammary gland during lactogenesis

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Summary

The ultrastructural development of the rabbit epithelial mammary gland cell is described during:

The appearance of secretory phenomena usually occuring in primiparous rabbits in the last third of pregnancy.

Experimental lactogenesis induced by injecting pseudopregnant rabbits with prolactin.

The ultrastructure of the active secretory (lactating) rabbit mammary gland cell is distinguished from that of inactive epithelial cells (beginning of pregnancy or pseudo-pregnancy) by: a considerable development of the ergastoplasm organized in parallel or concentric lamellae; hypertrophy of the Golgi apparatus; the presence of protein granules included in vacuoles of Golgian origin in the middle of hypertrophied cytoplasm. The presence of lipid droplets is less indicative of an active secretory state.

The induction of milk secretion during normal gestation or after a prolactin injection produces ultrastructural modifications in the mammary gland cell which are characterized by progressive development of the ergastoplasmic membrane system. This transformation becomes evident between 12–24 hours and on the third day of hormonal administration resembles that of the lactating mammary gland. The formation of a large endoplasmic reticulum to which the majority of ribosomes are bound and the development of cisterns and Golgi vacuoles appear under the influence of prolactin. The signification of the temporary existence, during the early stage of lactogenesis, of a vacuolar ergastoplasmic organization, is discussed.

Résumé

L'évolution de l'ultrastructure de la cellule épithéliale mammaire de la Lapine est décrite au cours de:

L'apparition des phénomènes sécrétoires qui prennent place normalement pendant le premier tiers de la gestation des Lapines primipares.

La lactogénèse expérimentale produite par des injections de prolactine à des Lapines pseudo-gestantes.

L'ultrastructure de la cellule mammaire de Lapine en sécrétion active (lactation) se distingue de celle des cellules inactives pour la sécrétion (début de la gestation et pseudogestation) par: le développement considérable de l'ergastoplasme organisé en lamelles parallèles ou concentriques; l'hypertrophie de l'appareil de Golgi; la présence de granules protéiques inclus dans des vacuoles d'origine golgienne au sein d'un cytoplasme hypertrophié, celle des gouttelettes lipidiques étant moins indicatrice d'un état d'activité sécrétoire.

L'induction de la sécrétion lactée au cours de la gestation normale ou à la suite de l'injection de prolactine, conduit à des modifications ultrastructurales de la cellule mammaire qui se caractérisent principalement par le développement progressif des systèmes membranaires ergastoplasmiques, qui est déjà net entre 12 et 24 heures et aboutit le 3e jour de l'administration hormonale à des images se rapprochant de celles d'une cellule de glande mammaire en lactation. La formation d'un important réticulum endoplasmique auquel est liée la majorité des ribosomes, ainsi que le développement des citernes et vacuoles golgiennes, paraissent ainsi sous le contrôle de la prolactine. Nous discutons enfin de la signification de l'existence transitoire dans les premiers stades de la lactogénèse d'une organisation vacuolaire de l'ergastoplasme.

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Authors and Affiliations

  1. Laboratoire de Physiologie de la Lactation, Institut National de la Recherche Agronomique C.N.R.Z., Jouy-en-Josas

    M. Bousquet, J. E. Fléchon & R. Denamur

  2. Station Centrale de Physiologie Animale, Institut National de la Recherche Agronomique C.N.R.Z., Jouy-en-Josas

    M. Bousquet, J. E. Fléchon & R. Denamur

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  1. M. Bousquet
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  2. J. E. Fléchon
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  3. R. Denamur
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Les tirages photographiques sont dus à Messieurs Scandolo et Amar que nous remercions vivement.

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Bousquet, M., Fléchon, J.E. & Denamur, R. Aspects ultrastructuraux de la glande mammaire de lapine pendant la lactogénèse. Z. Zellforsch. 96, 418–436 (1969). https://doi.org/10.1007/BF00335217

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