Skip to main content
SpringerLink
Log in

Phospholipidgranula im verhornenden Oesophagusepithel

  • Published:
Zeitschrift für Zellforschung und Mikroskopische Anatomie Aims and scope Submit manuscript

Summary

Lamellated granules of 0,1 to 0,3 μ in diameter are consistently found in all strata of the keratinizing epithelium of the guinea pig oesophagus. The granules are surrounded by a single membrane (unit membrane) and contain a lamellated system consisting of dark and light bands showing a periodicity of 60 to 70 Å. The supposed phospholipid nature of the granules was supported by the positive Baker-reaction at the light microscope level. The distribution of the Baker-positive substance was identical with that of the lamellated granules at the electron micrograph. After extraction with pyridin, the Baker-reaction turned out to be negative while on the electron micrographs the substance of the lamellated granules was lost.

The granules first appear near the Golgi region of the stratum germinativum and are emptied into the extra-cellular space at the level of the stratum granulosum. The substance of the granules, after having lost their lamellated structure, remains between the keratinized layers as a homogenous, dense material. Its function probably consists in increasing the resistance of the cornified layer against chemical agents.

Zusammenfassung

Im Epithel des Meerschweinchenoesophagus fanden wir mit Ausnahme des Stratum corneum in jeder Schicht 0,1–0,3 μ große, lamellär-strukturierte Granula. Die Granula werden meistens von einer Membran (unit-membrane) umgeben, die ein lamelläres System in sich einschließt, das eine Periodizität von 60–70 Å aufweist und aus dunklen und hellen Lamellen besteht. Unsere Annahme, daß die Granula Phospholipide enthalten, wird durch die Beobachtung unterstützt, daß die Lokalisation der lichtmikroskopisch durchgeführten Baker-Reaktion vollkommen mit der Verteilung der lamellären Granula im elektronenmikroskopischen Bild übereinstimmt. Nach Pyridinextraktion ist die Baker-Reaktion negativ, während im elektronenmikroskopischen Material an Stelle der Granula nur Vakuolen zu finden sind.

Die Granula erscheinen im Stratum germinativum meistens in Verbindung mit dem Golgiapparat und entleeren sich in Höhe des Stratum granulosum in den interzellulären Raum. Zwischen den Lamellen des Stratum corneum ist das Material der Granula als eine homogene, dunkle, amorphe Deckschicht vorhanden. Ihre Aufgabe besteht wahrscheinlich in der Steigerung der Resistenz der Hornschicht.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literatur

  • Barka, T., and P. J. Anderson: Histochemistry. New York: Harper and Row 1963.

    Google Scholar 

  • Brody, I.: An electron microscopic investigation of the keratinization process in the epidermis. Acta derm.-venerol. (Stockh.) 40, 74–84 (1960).

    Google Scholar 

  • Eckstein, H. C., and U. J. Wile: The cholesterol and phospholipid content of the cutaneous epithelium of man. J. biol. Chem. 69, 181–186 (1926).

    Google Scholar 

  • Elbers, P. F., J. T. Ververgaert, and R. Demel: Tricomplex fixation of phospholipids. Cell Biol. 24, 23–30 (1965).

    Google Scholar 

  • —: Electron microscope and X-ray diffraction studies on a homologous series of saturated phosphatidylcholines. Cell Biol. 25, 375–378 (1965).

    Google Scholar 

  • Farbman, A. I.: Electron microscope study of a small cytoplasmic structure in rat oral epithelium. J. Cell Biol. 21, 491–495 (1964).

    Google Scholar 

  • Finean, J. B.: Electron microscope and X-ray diffraction studies of a saturated synthetic phospholipid. J. biophys. biochem. Cytol. 6, 123 (1959).

    Google Scholar 

  • Fraser, R. D. B., T. P. MacRae, G. E. Rogers, and B. K. Filshie: Lipids in keratinized tissues. J. molec. Biol. 7, 90–91 (1963).

    Google Scholar 

  • Frei, J. V., and H. Sheldon: A small granular component of the cytoplasm of keratinizing epithelia. J. biophys. biochem. Cytol. 11, 719–724 (1961).

    Google Scholar 

  • Horstmann, E.: Die Haut. In: Handbuch der mikroskopischen Anatomie des Menschen, herausgeg. von W. Bargmann, Bd. III/3. Berlin-Göttingen-Heidelberg: Springer 1957.

    Google Scholar 

  • — und A. Knoop: Elektronenmikroskopische Studien an den Epidermis I. Rattenpfote. Z. Zellforsch. 47, 348–362 (1958).

    Google Scholar 

  • Jarrett, A.: In: A. Brook, (ed.), Progress in the biological sciences in relation to dermatology, p. 135. London and New York: Cambridge Univ. Press 1960.

    Google Scholar 

  • Kooyman, D. J.: Lipids of the skin. LXI. Some changes in the lipids of the epidermis during the process of keratinization. Arch. Derm. Syph. (Chic.) 25, 444–450 (1932).

    Google Scholar 

  • Lars, F., and J. Wersäll: A highly ordered structure in keratinizing human oral epithelium. J. Ultrastruct. Res. 12, 371–379 (1965).

    Google Scholar 

  • Luzzati, V., and F. Husson: The structure of liquid-crystalline phases of lipid-water systems. J. biophys. biochem. Cytol. 12, 207–219 (1962).

    Google Scholar 

  • Matoltsy, A. G., and P. F. Parakkal: Membran-coating granules of keratinizing epithelia. J. Cell Biol. 24, 297–307 (1965).

    Google Scholar 

  • Millonig, G.: Advantages of phosphate buffer for OsO4 solutions in fixation. J. appl. Phys. 32, 1637 (1961).

    Google Scholar 

  • Montagna, W.: The structure and function of skin. New York: Academic Press 1956.

    Google Scholar 

  • Nix jr., T. E., R. E. Nordquit, and M. A. Everett: Ultrastructural changes induced by ultraviolet light in human epidermis: Granular and transitional cell layers. J. Ultrastruct. Res. 12, 547–573 (1965).

    Google Scholar 

  • Odland, G. F.: A submicroscopic granular component in human epidermis. J. invest. Derm. 34, 11–15 (1960).

    Google Scholar 

  • Oláh, I., and Ottilia Török: Comparative examination of keratinization of Hassall's corpuscles of entodermal origin and of oesophagus epithelium on guinea pig. Acta biol. Acad. Sci. hung. 16, 353–368 (1966).

    Google Scholar 

  • Reynolds, E. S.: The use of lead citrate at high pH as an electronopaque stain in electron microscopy. J. Cell Biol. 17, 208–212 (1963).

    Google Scholar 

  • Röhlich, P.: Nicht veröffentlichte Angaben (1964).

  • Selby, C. C.: An electron microscope study of thin sections of human skin. II. Superficial cell layers of footpad epidermis. J. invest. Derm. 29, 131–149 (1957).

    Google Scholar 

  • Sheldon, H., and H. Zetterquist: Experimentally induced changes in mitochondrial morphology: Vitamin A deficiency. Exp. Cell Res. 10, 225–228 (1956).

    Google Scholar 

  • Snider, B. L., H. R. Gottschalk, and S. Rothman: The fate of choline in normal and pathologic keratinization of the epidermis. J. invest. Derm. 13, 323–324 (1949).

    Google Scholar 

  • Stempak, J. G., and R. T. Word: An improved staining method for electron microscopy. J. Cell Biol. 22, 697–701 (1964).

    Google Scholar 

  • Stoeckenius, W.: An electron microscope study of myelin figures. J. biophys. biochem. Cytol. 5, 491–500 (1959).

    Google Scholar 

  • —: Osmium tetroxide fixation of lipids. Proc. Europ. Reg. Conf. Electron Micr. Delft 2, 716 (1960).

    Google Scholar 

  • —: Some electron microscopical observations on liquid-crystalline phases in lipid-water systems. J. biophys. biochem. Cytol. 12, 221–229 (1962).

    Google Scholar 

  • Swanbeck, G.: Macromolecular organization of epidermal keratin. Acta derm.-venerol. (Stockh.) 39, Suppl. 1–37 (1959).

    Google Scholar 

  • Szodoray, L.: Beiträge zur Eiweißstructur des Hautepithels. Arch. Derm. Syph. (Berl.) 159, 605–610 (1930).

    Google Scholar 

  • Wislocki, G. B.: The staining of the intercellular bridges of the stratified squamous epithelium of the oral and vaginal mucosa by sudan black B and Baker's hematein method. Anat. Rec. 109, 388 (1951).

    Google Scholar 

  • Zelickson, A. S., and J. F. Hartmann: An electron microscopic study of human epidermis. J. invest. Derm. 36, 65–72 (1961).

    Google Scholar 

  • —: An electron microscope study of normal human non-keratinizing oral mucosa. J. invest. Derm. 38, 99–107 (1962).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Histologisch-Embryologisches Institut der Medizinischen Universität Budapest, Hungary

    I. Oláh & P. Röhlich

Authors
  1. I. Oláh
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. P. Röhlich
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Oláh, I., Röhlich, P. Phospholipidgranula im verhornenden Oesophagusepithel. Zeitschrift für Zellforschung 73, 205–219 (1966). https://doi.org/10.1007/BF00334864

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00334864

Search

Navigation