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Effect of dithiocarb and dimethyl sulfoxide on irreversible binding of 14C-bromobenzene to rat liver microsomal protein

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Abstract

The irreversible binding of [14C]-bromobenzene to rat liver microsomal protein in vitro was inhibited by dithiocarb and DMSO. Dithiocarb suppressed this binding in a time- and concentration-dependent manner (I50 = 4.5 10−5 M). DMSO reduced the degree of covalent binding by 61% from 5 x 10−5 M to 8 × 10−4 M. Dithiocarb was also effective in inhibiting irreversible binding of bromobenzene to liver protein in vivo. Our results are consistent with the hypothesis that dithiocarb exerts its antihepatotoxic efficacy by depressing microsomal mixed-function oxidase activity.

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Authors and Affiliations

  1. Institut für Toxikologie der Medizinischen Hochschule Lübeck, D-2400, Lübeck, Federal Republic of Germany

    M. Younes, C. -P. Siegers & J. G. Filser

  2. Institut für Toxikologie der Universität Tübingen, D-7400, Tübingen, Federal Republic of Germany

    J. G. Filser

Authors
  1. M. Younes
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  2. C. -P. Siegers
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  3. J. G. Filser
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Younes, M., Siegers, C.P. & Filser, J.G. Effect of dithiocarb and dimethyl sulfoxide on irreversible binding of 14C-bromobenzene to rat liver microsomal protein. Arch Toxicol 42, 289–293 (1979). https://doi.org/10.1007/BF00334843

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  • DOI: https://doi.org/10.1007/BF00334843

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