Summary
The transmissible plasmid pAMβ1, which codes for resistance to erythromycin and lincomycin, was transferred from Streptococcus faecalis to several strains of Bacillus thuringiensis by a filter-mating process. Introduction of pAMβ1 into the Emr transconjugant strains of B. thuringiensis was confirmed by Southern hybridisation using the 32P-labelled pAMβ1 as a probe. In the B. thuringiensis transconjugant strains, used as donors, the β plasmid conserved its ability to be transferred during intraspecific mating, with a frequency of 10-4 per recipient cell. In addition, the transconjugant clones acted as donors of the erythromycin resistance marker and permitted the transfer of cryptic plasmids present in the B. thuringiensis (β) strains used as donors. From a transconjugant clone of B. thuringiensis a hybrid plasmid resulting from an in vivo insertion into pAMβ1 of a 3 Md DNA sequence was isolated. This 3 Md DNA molecule originated from a 54 Md plasmid of a kurstaki strain and is related to several plasmids found in different serotypes of B. thuringiensis.
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Abbreviations
- cry− :
-
acrystalliferous mutant
- CCC:
-
covalently closed circular DNA
- Md:
-
megadalton
- EMS:
-
ethyl methanesulphonate
References
Barjac H de (1981) Identification of H. serotypes of Bacillus thuringiensis. In: Burges HD (ed) Microbial control of pest and plant deseases, 1970–1980. Academic Press Inc, New York, p 35
Birnboim HC, Doly J (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucl Acids Res 7:1513–1523
Chang S, Cohen SN (1979) High frequency transformation of Bacillus subtilis protoplasts. Mol Gen Genet 168:111–115
Clewell DB, Yagi Y, Dunny CM, Schultz SK (1974) Characterization of three plasmid deoxyribonucleic acid molecules in a strain of Streptococcus faecalis: identification of a plasmid determining erythromycin resistance. J Bacteriol 117:283–289
Gonzalez JM Jr, Carlton BC (1980) Patterns of plasmid DNA in crystalliferous and acrystalliferous strains of Bacillus thuringiensis. Plasmid 3:92–98
Gonzalez JM Jr, Carlton BC (1982) Plasmid transfer in Bacillus thuringiensis. In: Streips UN, Goodgal SH, Guild WR, Wilson GA (eds) Genetic exchange: a celebration and a new generation. Dekker, New York, p 85
Gonzalez JM Jr, Brown BJ, Carlton BC (1982) Transfer of Bacillus thuringiensis plasmids coding for δ-endotoxin among strains of B. thuringiensis and B. cereus. Proc Nat Acad Sci USA 79:6951–6955
Held GA, Bulla LA Jr, Ferrari E, Hoch J, Aronson AI, Munnich SA (1982) Cloning and localization of the lepidopteran protoxin gene of Bacillus thuringiensis subsp. kurstaki. Proc Nat Acad Sci USA 79:6065–6069
Jacob AE, Hobbs SJ (1974) Conjugal transfer of plasmid-borne multiple antibiotic resistance in Streptococcus faecalis var. zymogenes. J Bacteriol 117:360–372
Klier AF, Lecadet M-M, Dedonder R (1973) Sequential modification of DNA dependant RNA polymerase during sporogenesis in Bacillus thuringiensis. Eur J Biochem 36:317–327
Klier A, Fargette F, Ribier J, Rapoport G (1982) Cloning and expression of the crystal protein genes from Bacillus thuringiensis strain berliner 1715. EMBO J 1:791–799
Kreft J, Bernhardt K, Goebel W (1978) Recombinant plasmids capable of replication in B. subtilis and E. coli. Molec Gen Genet 162:59–67
Landman OE, Pepin RA (1982) B. subtilis mating system: dicaryotic recombinants and suppression of the prototrophic phenotype. In: Ganesan AT, Chang S, Hoch JA (eds) Molecular cloning and gene regulation in bacilli. Academic Press Inc., New York, p 25
Leblanc DJ, Hawley RJ, Lee LN, St Martin EJ (1978) “Conjugal” transfer of plasmid DNA among oral streptococci. Proc Nat Acad Sci USA 75:3484–3487
Leblanc DJ, Lee NL, Donkerslott JA, Harr RJ (1982) Plasmid transfer in Streptococci. In: Schlessinger D (ed) Microbiology. Am Soc for Microbiology, Washington DC, p 82
Lecadet M-M, Blondel M-O, Ribier J (1980) Generalized transduction in Bacillus thuringiensis var. berliner 1715, using bacteriophage CP54 Ber. J Gen Microbiol 121:203–212
Lecadet M-M, Lereclus D, Blondel M-O, Ribier J (1981) Bacillus thuringiensis: studies on chromosomal and extrachromosomal DNA. In: Levinson HS, Sonenshein AL, Tipper DJ (Eds) Sporulation and germination. Am Soc for Microbiology, Washington DC, p 88
Lereclus D, Lecadet M-M, Ribier J, Dedonder R (1982) Molecular relationships among plasmids of Bacillus thuringiensis: conserved sequences through 11 crystalliferous strains. Mol Gen Genet 186:391–398
Martin PAW (1982) Gene transfer in the insect pathogen, Bacillus thuringiensis. Abstracts P57:8. In: XIII International congress of microbiology. Am Soc for Microbiology. Boston, Mass (USA).
Rigby PWJ, Dieckmann M, Rhodes C, Berg P (1977) Labelling deoxyribonucleic acid to high specific activity in vitro by nick-translation with DNA polymerase I. J Molec Biol 113:237–251
Schaberg DR, Clewell DB, Glatzer L (1982) Conjugative transfer of R. plasmids from Streptococcus faecalis to Staphylococcus aureus. Antimicrobial agents and chemotherapy 22:204–207
Schnepf HE, Whiteley HR (1981) Cloning and expression of the Bacillus thuringiensis crystal protein gene in Escherichia coli. Proc Nat Acad Sci USA 78:2893–2897
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Communicated by A. Böck
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Lereclus, D., Menou, G. & Lecadet, MM. Isolation of a DNA sequence related to several plasmids from Bacillus thuringiensis after a mating involving the Streptococcus faecalis plasmid pAMβ1. Mol Gen Genet 191, 307–313 (1983). https://doi.org/10.1007/BF00334831
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DOI: https://doi.org/10.1007/BF00334831