Skip to main content
SpringerLink
Log in

Mapping of the genes for Bacillus subtilis ribosomal proteins S9, S11, and BL27 by means of antibiotic resistant mutants

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

The genes for Bacillus subtilis ribosomal proteins S9, S11, and BL27 were mapped using mutants with electrophoretic variants of these proteins isolated for their kasugamycin resistant phenotype. All three genes were in the principal cluster of ribosomal protein genes, and were clockwise to rpsE. BL27 very probably corresponds to Escherichia coli protein L30, so in B. subtilis the positions of the gene for this protein and of that for protein S11 are very similar with respect to the S5 (and S12) genes to the positions in E. coli. In contrast, whereas the S9 gene is in the main cluster in B. subtilis it is elsewhere on the chromosome in E. coli. Taken together with the fact that the genes of the Rif cluster are close to rpsL in B. subtilis, it appears that ribosomal protein genes are less dispersed in this organism than in E. coli. The same has been reported to be the case for the rRNA genes. Mutations in rpsI, the gene for protein S9, can confer a kasugamycin resistant phenotype on the cell.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bachmann BJ, Low KB (1980) Linkage map of Escherichia coli K-12, edition 6. Microbiol Rev 44:1–56

    Google Scholar 

  • Dabbs ER (1979) Selection for Escherichia coli mutants with proteins missing from the ribosome. J Bacteriol 140:734–737

    Google Scholar 

  • Dabbs ER (1982) Selection in Bacillus subtilis giving rise to strains with mutational alterations in a variety of ribosomal proteins. Mol Gen Genet 187:297–301

    Google Scholar 

  • Dabbs ER (1983) Dependence on kasugamycin arising from mutation at the rpsI locus, the gene for ribosomal protein S9, which maps at about 70 min on the Escherichia coli linkage map. J Bacteriol 153:709–715

    Google Scholar 

  • Davis BD, Mingioli ES (1950) Mutants of Escherichia coli requiring methionine or vitamin B12. J Bacteriol 60:17–28

    Google Scholar 

  • Garvin RT, Gorini L (1975) A new gene for ribosomal restriction in Escherichia coli. Mol Gen Genet 137:73–78

    Google Scholar 

  • Harford N, Sueoka N (1970) Chromosomal location of antibiotic resistance markers in Bacillus subtilis. J Mol Biol 51:267–286

    Google Scholar 

  • Henner DJ, Hoch JA (1980) The Bacillus subtilis chromosome. Microbiol Rev 44:57–82

    Google Scholar 

  • Higo K, Otaka E, Osawa S (1982) Purification and characterization of 30S ribosomal proteins from Bacillus subtilis: correlation to Escherichia coli 30S proteins. Mol Gen Genet 185:239–244

    Google Scholar 

  • Itoh T, Kosugi H, Higo K, Osawa S (1973) Genetic and chemical studies of ribosomes from spectinomycin resistant mutants of Bacillus subtilis. Mol Gen Genet 124:107–115

    Google Scholar 

  • Jaskunas SR, Fallon AM, Nomura M (1977) Identification and organisation of ribosomal protein genes of Escherichia coli carried on fus2 transducing phage. J Biol Chem 252:7323–7336

    Google Scholar 

  • Kimura A, Kobata K, Takata R, Osawa S (1975) Ribosomal proteins from streptomycin resistant and dependent mutants, and revertants from a streptomycin dependent mutant in Bacillus subtilis. Mol Gen Genet 139:293–301

    Google Scholar 

  • Okuyama A, Machiyama N, Kinoshita T, Tanaka W (1971) Inhibition by kasugamycin of initiation complex formation on 30S ribosomes. Biochem Biophys Res Commun 43:196–199

    Google Scholar 

  • Osawa S, Tokui A (1978) Mapping by interspecies transformation experiments of several ribosomal protein genes near the replication origin of the Bacillus subtilis chromosome. Mol Gen Genet 164:113–129

    Google Scholar 

  • Pai YL, Dabbs ER (1981) Conditional lethal mutants of Bacillus subtilis dependent on kasugamycin for growth. Mol Gen Genet 183:478–483

    Google Scholar 

  • Sillers IY, Moore PB (1981) Position of protein S1 in the 30S ribosomal subunit of Escherichia coli. J Mol Biol 153:761–780

    Google Scholar 

  • Tanaka K, Tamaki M, Osawa S (1973) Erythromycin resistant mutants of Bacillus subtilis. Mol Gen Genet 127:157–161

    Google Scholar 

  • Tominaga A, Kobayashi Y (1979) Kasugamycin resistant mutants of Bacillus subtilis. J Bacteriol 135:1149–1150

    Google Scholar 

  • Wienen B, Ehrlich R, Stöffler-Meilicke M, Stöffler G, Smith I, Weiss D, Vince R, Pestka S (1979) Ribosomal protein alterations in thiostrepton and micrococcin resistant mutants of Bacillus subtilis. J Biol Chem 254:8031–8041

    Google Scholar 

  • Wilson FE, Hoch JA, Bott K (1981) Genetic mapping of a linked cluster of ribonucleic acid genes in Bacillus subtilis. J Bacteriol 148:624–628

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Abt. Wittmann, Max-Planck-Institut für Molekulare Genetik, Ihnestraße 63-73, D-1000, Berlin 33

    Eric R. Dabbs

Authors
  1. Eric R. Dabbs
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by A. Böck

Rights and permissions

Reprints and permissions

About this article

Cite this article

Dabbs, E.R. Mapping of the genes for Bacillus subtilis ribosomal proteins S9, S11, and BL27 by means of antibiotic resistant mutants. Mol Gen Genet 191, 295–300 (1983). https://doi.org/10.1007/BF00334829

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00334829

Keywords

Search

Navigation