Summary
Distribution of glucose-6-phosphate dehydrogenase (G6PD) and 6-phospho-gluconate dehydrogenase (6PGD) in imaginal discs of Drosophila melanogaster was determined. Differential patterns of staining were found in all discs examined, i.e., eye-antennal, wing, leg, labial and genital. By using null mutants for either G6PD or 6PGD, the enzymes were shown to have the same distribution patterns. Staining with glucose-6-phosphate as a substrate resulted in the detection of both G6PD and 6PGD. Results of staining discs from homoeotic mutants indicate that the enzyme distribution patterns are under genetic control. In the presence of the homoeotic engrailed (en) mutation which transforms posterior wing compartment into anterior, the G6PD pattern of the posterior compartment of the wing disc was specifically transformed toward that of the anterior compartment. The bithorax series of homoeotic mutants was similarly investigated. The bithorax (bx 3) mutation transforms the anterior part of the haltere to anterior wing blade. Similarly the G6PD pattern in the anterior haltere disc transforms to that of anterior wing disc. The complimentary transformation, postbithorax (pbx) results in a change of the posterior part of the haltere to posterior wing, which is likewise reflected in an altered staining pattern for G6PD in the posterior portion of the haltere disc. The combination of the bx 3 and pbx resulted in a staining pattern of the haltere disc virtually indistinguishable from the normal wing disc.
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References
Bewley GC, Lucchesi JC (1975) Lethal effects of low and null activity alleles of 6-phosphogluconate dehydrogenase in Drosophila melanogaster. Genetics 79:451–466
Bryant PJ (1975) Pattern formation in the imaginal wing disc of Drosophila melanogaster: fate map, regeneration and duplication. J Exp Zool 193:49–78
Campos-Ortega JA, Waitz M (1978) Cell clones and pattern formation: Developmental restrictions in the compound eye of Drosophila. Wilhelm Roux' Archiv 184:155–170
Cunningham GN, Kuhn DT (1981) Tissue distribution of isocitrate dehydrogenase in Drosophila melanogaster. Insect Biochem 11:277–285
Cypher JJ, Tedesco JL, Courtright JB, Kumaran AK (1982) Tissuespecific and substrate-specific detection of aldehyde and pyridoxal oxidase in larval and imaginal tissues of Drosophila melanogaster. Biochem Genet 20:315–332
Deak II (1977) A histochemical study of the muscle of Drosophila melanogaster. J Morphol 153:307–316
Dickinson WJ (1971) Aldehyde oxidase in Drosophila melanogaster. A system for genetic studies on developmental regulation. Dev Biol 26:77–86
Ephrussi B, Beadle GW (1936) A technique of transplantation for Drosophila. Am Nat 70:218–225
Fox DJ, Conscience-Egli M, Abacherli E (1972) The soluble citric acid cycle enzymes of Drosophila melanogaster. II. Tissue and intracellular distribution of aconitase and NADP-dependent isocitrate dehydrogenase. Biochem Genet 7:163–175
Garcia-Bellido A, Ripoll P, Morata G (1973) Developmental compartmentalization in the wing disc of Drosophila. Nature (Lond) New Biol 245:251–253
Gerasimova TI, Smirnova SG (1979) Maternal effect for genes encoding 6-phosphogluconate dehydrogenase and glucose-6-phosphate dehydrogenase in Drosophila melanogaster. Dev Genetics 1:97–107
Gvozdev VA, Gerasimova TI, Kogan GL, Braslavskaya OY (1978) Role of the pentose cycle in the metabolism of Drosophila melanogaster, detected with the aid of mutations that inactivate glucose-6-phosphate and 6-phosphogluconate dehydrogenases. GEBS Letter 64:85–94
Henderson B, Loveridge N, Robertson WR (1978) A quantitative study of the effects of different grades of polyvinyl alcohol on the activities of certain enzyme in unfixed tissue sections. Histochemical J 10:453–463
Hughes B, Lucchesi JC (1978) Dietary rescue of a lethal null activity allele of 6-phosphogluconate dehydrogenase in Drosophila melanogaster. Biochem Genet 16:469–476
Janning W (1973) Distribution of aldehyde oxidase activity in imaginal discs of Drosophila melanogaster. Drosophila Inf Serv 50:151–152
Kankel DS, Hall JC (1976) Fate mapping of nervous system and other internal tissues in genetic mosaics of Drosophila melanogaster. Dev Biol 48:1–24
Kuhn DT, Cunningham GN (1976) Aldehyde oxidase activity in the tumorous-head strain of Drosophila melanogaster. Dev Biol 52:43–51
Kuhn DT, Cunningham GN (1977a) Aldehyde oxidase compartmentalization in Drosophila melanogaster wing imaginal disks. Science 196:875–877
Kuhn DT, Cunningham GN (1977b) Aldehyde oxidase distribution in haltere discs of homoeotic bithorax mutants in Drosophila melanogaster. Mol Gen Genet 150:37–42
Kuhn DT, Cunningham GN (1978) Aldehyde oxidase distribution in Drosophila melanogaster mature imaginal discs, histoblasts and rings of imaginal cells. J Exp Zool 204:1–10
Lewis EB (1978) A gene complex controlling segmentation in Drosophila. Nature 276:565–570
Lindsley DL, Grell EH (1968) Genetic variations of Drosophila melanogaster Carnegie Inst Washington, DC
MacIntyre RJ, O'Brien SJ (1976) Interacting gene-enzyme system in Drosophila. Annu Rev Genet 10:218–318
Ouweneel W, Van der Meer JM (1973) Differentiation capacities of the dorsal metathoracic (haltere) disc of Drosophila melanogaster. I. Normal organ map. Wilhelm Roux' Arch 172:149–161
Ready DF, Hanson TE, Benzer S (1976) Development of the Drosophila retina, a neurocrystalline lattice. Dev Biol 53:217–240
Sprey ThE (1977) Aldehyde oxidase distribution in the imaginal discs of some Diptera. Wilhelm Roux' Arch 183:1–15
Sprey ThE, Eskens AAC, Kuhn DT (1982) Enzyme distribution patterns in the imaginal wing disc of Drosophila melanogaster and other Diptera: A subdivision into territories. Wilhelm Roux' Arch 191:301–308
Sprey ThE, Segal D, Sprey-Pieters HE, Kuhn DT (1981) Influence of homoeotic genes on the aldehyde oxidase pattern in imaginal discs of Drosophila melanogaster. Dev Genet 2:75–87
Wright DA, Shaw CR (1970) Time of expression of genes controlling specific enzymes in Drosophila embryos. Biochem Genet 4:385–394
Weir MP, Lo CW (1982) Gap junction communication compartments in the Drosophila wing disc. Proc Natl Acad Sci USA 79:3232–3235
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Cunningham, G.N., Smith, N.M., Makowski, M.K. et al. Enzyme patterns in D. melanogaster imaginal discs: Distribution of glucose-6-phosphate and 6-phosphogluconate dehydrogenase. Mol Gen Genet 191, 238–243 (1983). https://doi.org/10.1007/BF00334820
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DOI: https://doi.org/10.1007/BF00334820