Skip to main content

DNA sequences of and complementation by the tnpR genes of Tn21, Tn501 and Tn1721

Summary

DNA sequences that encode the tnpR genes and internal resolution (res) sites of transposons Tn21 and Tn501, and the res site and the start of the tnpR gene of Tn1721 have been determined. There is considerable homology between all three sequences. The homology between Tn21 and Tn501 extends further than that between Tn1721 and Tn501 (or Tn21), but in the homologous regions, Tn1721 is 93% homologous with Tn501, while Tn21 is only 72–73% homologous. The tnpR genes of Tn21 and Tn501 encode proteins of 186 amino acids which show homology with the tnpR gene product of Tn3 and with other enzymes that carry out site-specific recombination. However, in all three transposons, and in contrast to Tn3, the tnpR gene is transcribed towards tnpA gene, and the res site is upstream of both. The res site of Tn3 shows no obvious homology with the res regions of these three transposons. Just upstream of the tnpR gene and within the region that displays common homology between the three elements, there is a 50 bp deletion in Tn21, compared to the other two clements. A TnpR derivative of Tn21 was complemented by Tn21, Tn501 and Tn1721, but not by Tn3.

This is a preview of subscription content, access via your institution.

References

  • Altenbuchner J, Choi CL, Grinsted J, Schmitt R, Richmond MH (1981) The transposons Tn501(Hg) and Tn1721(Tc) are related. Genet Res 37:285–289

    Google Scholar 

  • Altenbuchner J, Schmitt R (1983) Transposon Tn1721: site-specific recombination generates deletions and inversions. Mol Gen Genet 190:300–308

    Google Scholar 

  • Bennett PM, Grinsted J, Choi CL, Choi CL, Richmond MH (1978) Characterisation of Tn501, a transposon determining resistance to mercuric ions. Mol Gen Genet 159: 101–106

    Google Scholar 

  • Brown NL, Ford SJ, Pridmore RD, Fritzinger DC (1983) DNA sequence of a gene from the Pseudomonas transposon Tn501 encoding mercuric reductase. Biochemistry (in press)

  • de la Cruz F, Grinsted J (1982) Genetic and molecular characterization of Tn21, a multiple resistance transposon from R100.1 J Bacteriol 151:222–228

    Google Scholar 

  • Farabaugh PJ, Schmeissner U, Hofer M, Miller JH (1978) Genetic studies of the lac repressor. VII On the molecular nature of spontaneous hotspots in the lacI gene of Escherichia coli. J Mol Biol 126:847–863

    Google Scholar 

  • Grindley NDF, Lauth MR, Wells RG, Wityk RJ, Salvo JJ, Reed RR (1982) Transposon-mediated site-specific recombination: identification of three binding sites for resolvase at the res sites of γδ and Tn3. Cell 30:19–27

    Google Scholar 

  • Grinsted J, de la Cruz F, Altenbuchner J, Schmitt R (1982) Complementation of transposition of tnpA mutants of Tn3, Tn21, Tn501 and Tn1721. Plasmid 8:276–286

    Google Scholar 

  • Heffron F, Bedinger P, Champoux PP, Falkow S (1977) Deletions affecting the transposition of an antibiotic-resistance gene. Proc Nat Acad Sci USA 74:702–706

    Google Scholar 

  • Heffron F, McCarthy GJ, Ohtsubo H, Ohtsubo E (1979) DNA sequence analysis of the transposon Tn3: three genes and three sites involved in transposition of Tn3. Cell 18:1153–1163

    Google Scholar 

  • Kitts P, Symington D, Burke M, Reed R, Sherratt D (1982) Transposon-specified site-specific recombination. Proc Nat Acad Sci USA 79:46–50

    Google Scholar 

  • Kleckner N (1981) Transposable elements in procaryotes. Annu Rev Genet 15:341–404

    Google Scholar 

  • Konigsberg W, Godson GN (1983) Evidence for the use of rare codons in the dnaG gene and other regulatory genes in Escherichia coli. Proc Nat Acad Sci USA 80:687–691

    Google Scholar 

  • Maxam AM, Gilbert W (1977) A new method for sequencing DNA. Proc Nat Acad Sci USA 74:560–564

    Google Scholar 

  • Messing J, Crea R, Seeburg PH (1981) A system for shotgun DNA sequencing. Nucleic Acids Res 9:309–321

    Google Scholar 

  • Novick RP, Clowes RC, Cohen SN, Curtiss III R, Datta N, Falkow S (1976) Uniform nomenclature for bacterial plasmids: a proposal. Bacteriol Rev 40:168–189

    Google Scholar 

  • Reed RR, Grindley NDF (1981) Transposon-mediated site-specific recombination in vitro: DNA cleavage and protein-DNA linkage at the recombination site. Cell 25:721–728

    Google Scholar 

  • Reed RR, Shibuya GI, Steitz JA (1982) Nucleotide sequence of γδ resolvase gene and demonstration that its gene product acts as a repressor of transcription. Nature (London) 300:381–383

    Google Scholar 

  • Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Nat Acad Sci USA 74:5463–5467

    Google Scholar 

  • Schmitt R, Altenbuchner J, Grinsted J (1981a) Complementation of transposition functions encoded by transposon Tn501(Hg) and Tn1721(Tc). In: Levy SB, Clowes RC, Koenig EL (eds) Molecular biology, pathogenicity and ecology of bacterial plasmids. Plenum Publ Co, New York, pp 359–370

    Google Scholar 

  • Smitt R, Altenbuchner J, Wiebauer K, Arnold W, Pühler A, Schöffl F (1981 b) Basis of transposition and gene amplification by Tn1721 and related tetracycline-resistance transposons. Cold Spring Harbor Symp Quant Biol 45:59–65

    Google Scholar 

  • Schmitt R, Bernhard E, Mattes R (1979) Characterisation of Tn1721, a new transposon containing tetracycline resistance genes capable of amplification. Mol Gen Genet 172:53–65

    Google Scholar 

  • Schöffl F, Arnold W, Pühler A, Altenbuchner J, Schmitt R (1981) The tetracycline-resistance transposons Tn1721 and Tn1771 have three 38-base pair repeats and generate five base pair direct repeats. Mol Gen Genet 181:87–94

    Google Scholar 

  • Tse YS, Kirkegaard K, Wang JC (1980) Covalent bonds between protein and DNA. Formation of a phosphotyrosine linkage between certain DNA topoisomerases and DNA. J Biol Chem 255:5560–5565

    Google Scholar 

  • Zieg J, Simon M (1980) Analysis of the nucleotide sequence of an invertible controlling element. Proc Nat Acad Sci USA 77:4196–4200

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Communicated by A. Böck

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Diver, W.P., Grinsted, J., Fritzinger, D.C. et al. DNA sequences of and complementation by the tnpR genes of Tn21, Tn501 and Tn1721 . Mol Gen Genet 191, 189–193 (1983). https://doi.org/10.1007/BF00334812

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00334812

Keywords

  • Enzyme
  • Recombination
  • Gene Product
  • Homologous Region
  • Considerable Homology