Summary
Streptococcus thermophilus is a common dairy starter for which very few genetic exchange systems have been described. Here we report plasmid transduction by ϕ 17 α and ϕ56α, two virulent phages of industrial yogurt starter strains. Several replicons could be transduced, independently of their size, with efficiencies which are high enough to allow gene transfer from a transformable intermediary strain of S. thermophilus to other hosts of the bacteriophages studied.
This is a preview of subscription content, log in via an institution to check access.
References
Allen LK, Sandine WE, Elliker PR (1963) Transduction in Streptococcus lactis. J Dairy Res 30:351–357
Alonso JC, Lüder G, Trautner TA (1986) Requirements for the formation of plasmid-transducing particles of Bacillus subtilis bacteriophage SPP1. EMBO J 5:3723–3728
Davies FL, Gasson MJ (1981) Reviews of the progress of dairy science: genetics of lactic acid bacteria. J Dairy Res 48:363–376
Davies FL, Gasson MJ (1984) Bacteriophages of dairy lactic acid bacteria. In: Davies FL, Law BA (eds) Advances in the microbiology and biochemistry of cheese and fermented milks. Elsevier Applied Science Publishers, New York, pp 127–151
Deichelbohrer I, Alonso JC, Lüder G, Trautner TA (1985). Plasmid transduction by Bacillus subtilis bacteriophage SPP1: effects of DNA homology between plasmid and bacteriophage. J Bacteriol 162:1238–1243
De Man JC, Rogosa M, Sharp ME (1960) A medium for cultivation of lactobacilli. J Appl Bacteriol 23:130–135
Dyer DN, Rock MI, Lee CY, Iandolo JJ (1985) Generation of transducing particles in Staphylococcus aureus. J Bacteriol 161:91–95
Evans RP, Macrina FL (1983) Streptococcal R plasmid pIP501: endonuclease site map, resistant determinant location and construction of novel derivatives. J Bacteriol 154:1347–1355
Gasson MJ (1982) Identification of the lactose plasmid in Streptococcus lactis 712. In: Schlessinger D (ed) Microbiology 1982. American Society for Microbiology, Washington DC, pp 217–220
Gasson MJ (1983) Plasmid complements of Streptococcus lactis NCDO712 and other lactic streptococci after protoplast induced curing. J Bacteriol 154:1–9
Gasson MJ, Anderson PH (1985) High copy number plasmid vectors for use in lactic streptococci. FEMS Microbiol Lett 30:193–196
Lemoine Y, Courtney M, Lecocq JPL (1987) Fifth international conference on the genetics of industrial microorganisms, GIM 1986, Split. In: Alacevic M, Hranueli D, Toman Z (eds) Proceedings of the fifth international conference on the genetics of industrial microorganisms. Ognjen Prica Printing Works, Karlovac, Zagreb, Yugoslavia, pp 39–49
Macrina FL, Tobian JA, Jones KR, Evans RP, Clewell DB (1982) A cloning vector able to replicate in Escherichia coli and Streptococcus sanguis. Gene 19:345–353
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
McKay LL, Baldwin KA (1978) Stabilization of lactose metabolism in Streptococcus lactis C2. Appl Environ Microbiol 36:360–367
McKay LL, Cords BR, Baldwin KA (1973) Transduction of lactose metabolism in Streptococcus lactis C2. J Bacteriol 115:810–815
McKay LL, Baldwin KA, Efstathiou JD (1976) Transductional evidence for plasmid linkage of lactose metabolism in Streptococcus lactis C2. Appl Environ Microbiol 32:45–52
Mercenier A, Robert C, Romero DA, Slos P, Lemoine Y (1987) In: Ferretti JJ, Curtiss R III (eds) Streptococcal genetics. American Society for Microbiology, Washington, DC, pp 234–237
Murray NE, Brammar WJ, Murray K (1977) Lambdoïd phages that simplify the recovery of in vitro recombinants. Mol Gen Genet 150:53–61
Novick RP, Edelman I, Lofdahl S (1986) Small Staphylococcus aureus plasmids are transduced as linear multimers that are formed and resolved by replicative processes. J Mol Biol 192:202–220
Reinbold GN, Reddy MS, Hammond EG (1982) Ultrastructure of bacteriophages active against Streptococcus thermophilus, Lactobacillus bulgaricus, Lactobacillus lactis and Lactobacillus helveticus. J Food Protection 45:119–124
Romero DA, Slos P, Robert C, Castellino I, Mercenier A (1987) Conjugative mobilization as an alternative vector delivery system for lactic streptococci. Appl Environ Microbiol 53:2405–2413
Ruhfel RE, Robillard NJ, Thorne CB (1984) Interspecies transduction of plasmids among Bacillus anthracis, Bacillus cereus and Bacillus thuringiensis. J Bacteriol 157:708–711
Sandine WE, Elliker PR, Allen LK, Brown WC (1962) Genetic exchange and variability in lactic streptococcus starter organisms. J Dairy Sci 45:1266–1271
Snook RJ, McKay LL, Ahlstrand GG (1981) Transduction of lactose metabolism by Streptococcus cremoris C3 temperate phage. Appl Environ Microbiol 42:897–903
Terzaghi BE, Sandine WE (1975) Improved medium for lactic streptococci and their bacteriophages. Appl Environ Microbiol 29:807–813
Author information
Authors and Affiliations
Additional information
Communicated by H. Hennecke
Rights and permissions
About this article
Cite this article
Mercenier, A., Slos, P., Faelen, M. et al. Plasmid transduction in Streptococcus thermophilus . Mol Gen Genet 212, 386–389 (1988). https://doi.org/10.1007/BF00334713
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00334713