Summary
The plasmid pUB307, a derivative of RP1, is a conjugative, broad-host-range plasmid. We have shown that this element mobilizes gonococcal resistance plasmids from Escherichia coli to Neisseria gonorrhoeae, thus providing evidence that extrachromosomal elements can efficiently enter gonococci by conjugation. Furthermore, pUB307 can also be used as a helper element to mobilize the cloning vector pLES2 into N. gonorrhoeae. This finding significantly increases the usefulness of pLES2 as a shuttle vector between E. coli and gonococcus.
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References
Albritton WL, Brunton JL, Meier M, Bowman MN, Slaney LA (1982) Haemophilus influenzae: comparison of respiratory tract isolates with genitourinary tract isolates. J Clin Microbiol 16:826–831
Arini A, Peduzzi R, Piffaretti JC (1983) Epidémiologie des Neisseria gonorrhoeae isolées en Suisse: sensibilité aux antibiotiques et auxotypie. Schweiz Med Wochenschr 113:462–470
Bennett PM, Grinsted J, Richmond MH (1977) Transposition of TnA does not generate deletions. Mol Gen Genet 154:205–211
Biswas GD, Sox T, Blackman E, Sparling PF (1977) Factors affecting genetic transformation of Neisseria gonorrhoeae. J Bacteriol 129:983–992
Brunton J, Clare D, Meier MA (1986) Molecular epidemiology of antibiotic resistance plasmids of Haemophilus species and Neisseria gonorrhoeae. Rev Infect Dis 8:713–724
Eisenstein BI, Sox T, Biswas G, Blackman E, Sparling PF (1977) Conjugal transfer of the gonococcal penicillinase plasmid. Science 195:998–1000
Fayet O, Froment Y, Piffaretti JC (1982a) Beta-lactamase-specifying plasmids isolated from Neisseria gonorrhoeae have retained an intact right part of a Tn3-like transposon. J Bacteriol 149:136–144
Fayet O, Froment Y, Piffaretti JC (1982b) Analysis of the plasmid content of three beta-lactamase-producing Neisseria gonorrhoeae strains. FEMS Microbiol Lett 14:271–275
Flett F, Humphreys GO, Saunders JR (1981) Intra-specific and intergeneric mobilization of non-conjugative resistance plasmids by a 24.5 megadalton conjugative plasmid of Neisseria gonorrhoeae. J Gen Microbiol 125:123–129
Genco CA, Knapp JS, Clark VL (1984) Conjugation of plasmids of Neisseria gonorrhoeae to other Neisseria species: potential reservoirs for the beta-lactamase plasmid. J Infect Dis 150:397–401
Graves JF, Biswas GD, Sparling PF (1982) Sequence-specific DNA uptake in transformation of Neisseria gonorrhoeae. J Bacteriol 152:1071–1077
Guiney DG, Ito JI (1982) Transfer of the gonococcal penicillinase plasmid: mobilization in Escherichia coli by IncP plasmids and isolation as a DNA-protein relaxation complex. J Bacteriol 150:298–302
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
McNicol P, Albritton W, Ronald A (1986) Transfer of plasmid mediated ampicillin resistance from Haemophilus to Neisseria gonorrhoeae requires an intervening organism. Sex Transm Dis 13:145–150
Roberts M, Falkow S (1977) Conjugal transfer of R plasmids in Neisseria gonorrhoeae. Nature 266:630–631
Roberts M, Elwell LP, Falkow S (1977) Molecular characterization of two beta-lactamase-specifying plasmids isolated from Neisseria gonorrhoeae. J Bacteriol 131:557–563
Sox TE, Mohammed W, Blackman E, Biswas G, Sparling PF (1978) Conjugative plasmids in Neisseria gonorrhoeae. J Bacteriol 134:278–286
Sox TE, Mohammed W, Sparling PF (1979) Transformation-derived Neisseria gonorrhoeae plasmids with altered structure and function. J Bacteriol 138:510–518
Stein DC, Silver LE, Clark VL, Young FE (1983a) Construction and characterization of a new shuttle vector, pLES2, capable of functioning in Escherichia coli and Neisseria gonorrhoeae. Gene 2:241–247
Stein DC, Young FE, Tenover FC, Clark VL (1983b) Characterization of a chimeric beta-lactamase plasmid of Neisseria gonorrhoeae which can function in Escherichia coli. Mol Gen Genet 189:79–84
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Communicated by W. Arber
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Piffaretti, JC., Arini, A. & Frey, J. pUB307 mobilizes resistance plasmids from Escherichia coli into Neisseria gonorrhoeae . Mol Gen Genet 212, 215–218 (1988). https://doi.org/10.1007/BF00334687
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DOI: https://doi.org/10.1007/BF00334687