Summary
A temperature shift-up accompanied by a reduction in RNA polymerase activity in Escherichia coli causes an increased rate of initiation leading to a 1.7- to 2.2-fold increase in chromosome copy number. A temperature shift-up without a reduction in polymerase activity induces only a transient non-scheduled initiation of chromosome replication caused by heat shock with no detectable effect on chromosome copy number.
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References
Donachie WD (1968) Relationship between cell size and time of initiation of DNA replication. Nature 219:1077–2079
Fiil NP, Bendiak D, Collins J, Friesen JD (1979) Expression of E. coli ribosomal proteins and RNA polymerase genes cloned on plasmids. Mol Gen Genet 173:39–50
Gross CA, Engback F, Flammang T, Burgess R (1976) Rapid micromethod for the purification of E. coli RNA polymerase and the preparation of bacterial extracts active in RNA synthesis. J Bacteriol 128:382–389
Grossman AD, Erickson JW, Gross C (1984) The htpR gene product of E. coli is a sigma factor for heat-shock promoters. Cell 38:383–390
Grunstein M, Wallis J (1979) Colony hybridization. Methods Enzymol 68:379–389
Guzman EC, Jimenez Sanchez A (1986) Effect of multiple copies of rpoBC on the rate of RNA synthesis of Escherichia coli. Genet Res 48:61–64
Ishihama A, Fukuda R (1980) Autogenous and posttranscriptional regulation of RNA polymerase synthesis. Mol Cell Biochem 31:177–196
Koppes LJH, Woldringh CL, Nanninga N (1978) Size variation and correlation of different cell cycle events in slow-growing Escherichia coli. J Bacteriol 134:423–433
Light J, Molin S (1981) Replication control functions of plasmid R1 act as inhibitors of expression of a gene required for replication. Mol Gen Genet 184:56–61
Lother H, Buhk HJ, Morelli C, Heimann B, Chakraborty T, Messer W (1981) In: Ray DS (ed) Initiation of DNA replication. Academic Press, New York, pp 57–77
Pritchard RH, Barth PT, Collins J (1969) Control of DNA synthesis in bacteria. Symposium of the Society for General Microbiology, vol 19, pp 263–297
Pritchard RH (1985) Control of chromosome replication in bacteria. In: Helinski DR, Cohen SN, Clewell DB, Jackson DA, Hollaender A (eds) Plasmids in bacteria. Plenum Press, New York London, pp 277–282
Rasmussen KV, Atlung T, Kerszman G, Hansen GE, Hansen FG (1983) Conditional change of DNA replication control in an RNA polymerase mutant of Escherichia coli. J Bacteriol 154:443–451
Stougaard P, Molin S (1981) Vertical dye-bouyant density gradient analysis and preparation of plasmid DNA. Anal Biochem 118:191–193
Tanaka M, Ohmori H, Hiraga S (1983) A novel type of E. coli mutants with increased chromosomal copy number. Mol Gen Genet 192:51–60
Tomizawa J, Itoh T, Seizer G, Som T (1981) Inhibition of ColE1 RNA primer formation by a plasmid-specified small RNA. Proc Natl Acad Sci USA 78:1421–1425
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Communicated by W. Goebel
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Guzman, E.C., Jimenez-Sanchez, A., Orr, E. et al. Heat stress in the presence of low RNA polymerase activity increases chromosome copy number in Escherichia coli . Mol Gen Genet 212, 203–206 (1988). https://doi.org/10.1007/BF00334685
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DOI: https://doi.org/10.1007/BF00334685