Summary
The yeast invertase structural gene SUC2 has two naturally occurring alleles, the active one and a silent allele called suc2°. Strains carrying suc2° are unable to ferment sucrose and do not show detectable invertase activity. We have isolated suc2° and found an amber codon at position 232 of 532 amino acids. However, transformants carrying suc2° on a multicopy plasmid were able to ferment sucrose and showed detectable invertase activity. Full-length invertase was found in gels stained for active invertase and in immunoblots. Therefore we concluded that the amber codon is occasionally read as an amino acid. The calculated frequency of read-through is about 4% of all translation events.
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Communicated by C.P. Hollenberg
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Gozalbo, D., Hohmann, S. The naturally occurring silent invertase structural gene suc2° contains an amber stop codon that is occasionally read through. Mol Gen Genet 216, 511–516 (1989). https://doi.org/10.1007/BF00334398
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DOI: https://doi.org/10.1007/BF00334398