Summary
A complete SmaI, XhoI, BamHI restriction map of the maize mitochondrial genome from the T male sterile cytoplasm (cmsT) of maize has been established. The genome exists in the form of a complex multicircular structure as found for the maize normal (N) type (Lonsdale et al. 1984) where the entire sequence complexity with a content of 540 kb can be arranged on a single circular master chromosome. However, most of the repeats (inverted or direct) present in the maize cmsT genome are different from those found in the maize N genome. Recombinational events between these repeats generate a population of circular molecules rather different from the multipartite organization of the N genome. The mitochondrial genes are dispersed throughout the genome. The open reading frame coding for a 13 kDa polypeptide associated with cytoplasmic male sterility (Dewey et al. 1986, 1987) has also been located on the map.
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References
Bedinger P, De Hostos EL, Leon P, Walbot V (1986) Cloning and characterization of a linear 2.3 kb mitochondrial plasmid of maize. Mol Gen Genet 205:206–212
Braun CJ, Levings CS III (1985) Nucleotide sequence of the F1-ATPase alpha subunit gene from maize mitochondria. Plant Physiol 79:571–577
Chao S, Sederoff RR, Levings CS III (1983) Partial sequence analysis of the 5S and 18S rRNA gene region of the maize mitochondrial genome. Plant Physiol 71:190–192
Chao S, Sederoff R, Levings CS III (1984) Nucleotide sequence and evolution of the 18S ribosomal RNA gene in maize mitochondria. Nucleic Acids Res 12:6629–6644
Church GM, Gilbert W (1984) Genome sequencing. Proc Natl Acad Sci USA 81:1991–1995
Clark-Walker GD (1985) Basis of diversity in mitochondrial DNAs. In: Cavalier-Smith T (ed) The evolution of genome size. John Wiley & Sons, New York, pp 277–297
Dale RMK, Mendu N, Ginsburg H, Kridl JC (1984) Sequence analysis of themaize mitochondrial 26S rRNA gene and flanking regions. Plasmid 11:141–150
Davis RW, Botstein D, Roth JR (1980) Advanced bacterial genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, p 178
Dawson AJ, Jones VP, Leaver CJ (1984) The apocytochrome b gene in maize mitochondria does not contain introns and is preceded by a potential ribosome binding site. EMBO J 3:2107–2113
Dawson AJ, Hodge TP, Isaac PC, Leaver CJ, Lonsdale DM (1986) Location of the genes for cytochrome oxidase subunits I and II, apocytochrome b, subunit of the F1 ATPase and the ribosomal RNA genes on themitochondrial genome of maize (Zea Mays L.). Curr Genet 10:561–564
Dewey RE, Levings CS III, Timothy DH (1985a) Nucleotide sequence of ATPase subunit 6 gene of maize mitochondria. Plant Physiol 79:914–919
Dewey RE, Schuster AM, Levings CS III, Timothy DH (1985b) Nucleotide sequence of Fo-ATPase proteolipid (subunit 9) gene of maize mitochondria. Proc Natl Acad Sci USA 82:1015–1019
Dewey RE, Timothy DH, Levings CS III (1987) A mitochondrial protein associated with cytoplasmic male sterility in the T cytoplasm of maize. Proc Natl Acad Sci USA 84:5374–5378
Dewey RE, Levings CS III, Timothy DH (1986) Novel recombinations in the maize mitochondrial genome produce a unique transcriptional unit in the Texas male-sterile cytoplasm (cmsT). Cell 44:439–449
Fauron CM-R, Havlik M (1988) The BamHI, XhoI, SmaI restriction enzyme maps of the normal maize mitochondrial genome genotype B37. Nucleic Acids Res 16:10395–10396
Fauron CM-R, Havlik M (1989) The maize mitochondrial genomes of the normal type and the cytoplasmic male sterile type T have very different organization. Curr Genet (in press)
Fauron CM-R, Abbott AG, Brettell R, Gesteland R (1987) Maize mitochondrial DNA rearrangements between the normal type, the Taxas male sterile cytoplasm and a fertile revetant cmsT regenerated plant. Curr Genet 11:339–346
Fox TD, Leaver CJ (1981) The Zea mays mitochondrial gene coding cytochrome oxidase subunit II has an intervening sequence and does not contain TGA codons. Cell 26:315–323
Hohn B, Collins J (1980) A small cosmid for efficient cloning of large DNA fragments. Gene 11:291–298
Houchins, JP, Gisburg H, Rohrbaugh M, Dale RMK, Schardl CL, Hodge TP, Lonsdale DM (1986) DNA sequence analysis of a 5.27 kb direct repeat occurring adjacent to the regions of S episome homology in maize mitochondria. EMBO J 5:2781–2788
Isaac PG, Brennicke A, Dunbar SM, Leaver CJ (1985) The mitochondrial genome of fertile maize (Zea mays L.) contains two copies of the gene encoding the α-subunit of the F1-ATPase. Curr Genet 10:321–328
Kemble RJ, Bedbrook JR (1980) Low molecular weight circular and linear DNA in mitochondria from normal and male-sterile Zea mays cytoplasm. Nature 284:565–566
Kessler LG, Avise JC (1985) A comparative description of mitochondiral DNA differentiation in selected avian and other vertebrate genera. Mol Biol Evol 2:109–115
Leaver CJ and Gray MW (1982) Mitochondrial genome organization and expression in higher plants. Annu Rev Plant Physiol 33:373–402
Levings CS III, Shah DM, Hu WWL, Pring DR, Timothy DH (1979) Molecular heterogeneity among mitochondrial DNAs from different maize cytoplasms. In: Cummings DJ, Borst, P, Dawid I, Weissman, SM, Fox CF (eds) Extrachromosomal DNA. ICN-UCLA Symp Mol Cell Biol, pp 63–73
Lonsdale DM, Hodge TP, Fauron CM-R, Flavell RB (1983a) A predicted structure for the mitochondrial genome from the fertile cytoplasm of maize. In: Goldberg RB (ed) UCLA Symp Mol Cell Biol New Ser 12. Alan Liss, New York pp 445–456
Lonsdale DM, Hodge TP, Howe C, Stern B (1983b) Maize mitochondrial DNA contains a sequence homologous to the ribulose 1,5-bisphosphate carboxylase large subunit gene of chloroplast DNA. Cell 34:1007–1014
Lonsdale DM, Hodge TP, Fauron CM-R (1984) The physical map and organization of the mitochondrial genome from the fertile cytoplasm of maize. Nucleic Acids Res 12:9249–9261
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, pp 92–93
McCarty DM, Fejes E, Hauswirth WW (1986) Nucleotide sequence and transcription of the maize mitochondrial cytochrome oxidase subunit III gene. (Abstract) International workshop on higher plant mitochondrial DNA sponsored by the International Society for Plant Molecular Biology, Airlie House, p 38
Mulligan RM, Maloney AP, Walbot V (1988) RNA processing and multiple transcription initiation sites results in transcript size heterogeneity in maize mitochondria. Mol Gen Genet 211:373–380
Palmer JD (1988) Intraspecific variation and multicirularity in Brassica mitochondrial DNAs. Genetics 118:341–351
Palmer JD, Shields CR (1984) Tripartite structure of the Brassica campestris mitochondrial genome. Nature 307:437
Palmer JD, Herbon LA (1986) Tricircular mitochondrial genomes of Brassica and raphanus: reversal of repeat configurations by inversion. Nucleic Acids Res 14:9755–9764
Palmer JD, Herbon LA (1987) Unicircular structure of the Brassica Hirta mitochondrial genome. Curr Genet 11:565–570
Quetier F, Lejeune B, Delorme S, Falconet D, Jubier MF (1985) Molecular form and function of the wheat mitochondrial genome. In: Groot G, Hall T (eds) Molecular form and function of the plant genome, vol 83. Plenum Press, New York, pp 413–420
Siculella L, Palmer JD (1988) Physical and gene organization of mitochondrial DNA in fertile and male sterile sunflower. CMS-associated alterations in structure and transcription. Nucleic Acids Res 16:3787–3799
Small ID, Isaac PG, Leaver CJ (1987) Stoichiometric differences in DNA molecules containing the atpA gene suggest mechanisms for the generation of mitochondrial genome diversity in maize. EMBO J 6:865–869
Stamper SE, Dewey RE, Bland MM, Levings CS III (1987) Characterization of the gene urf13-T and an unidentified reading frame, ORF25, in maize and tobacco mitochondria. Curr Genet 12:457–463
Stern DB, Lonsdale DM (1982) Mitochondrial and chloroplast genomes of maize have a 12 kb DNA sequence in common. Nature 299:698–702
Stern DB, Palmer JD (1984) Extensive and widespread homologies between mitochondrial DNA and chloroplast DNA in plants. Proc Natl Acad Sci USA 81:1946–1950
Stern DB, Palmer JD (1986) Tripartite mitochondrial genome of spinach: Physical structure, mitochondrial gene mapping, and locations of transposed chloroplast DNA sequences. Nucleic Acids Res 14:5651–5666
Stern DB, Palmer JD, Thompson WF, Lonsdale DM (1983) Mitochondrial DNA sequence evolution and homology to chloroplast DNA in angiosperms. In: Goldberg RB (ed) UCLA Symp Mol Cell Biol New Ser 12. Alan Liss, New York, pp 467–477
Stern DB, Dyer TA, Lonsdale DM (1982) Organization of the mitochondrial ribosomal RNA genes of maize. Nucleic Acids Res 11:3333–3337
Ward BL, Anderson RS, Bendich AJ (1981) The mitochondrial genome is large and variable in a family of plants (Curcurbitaceae). Cell 25:792–803
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Communicated by R.G. Herrmann
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Fauron, C., Havlik, M., Lonsdale, D. et al. Mitochondrial genome organization of the maize cytoplasmic male sterile type T. Mol Gen Genet 216, 395–401 (1989). https://doi.org/10.1007/BF00334381
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DOI: https://doi.org/10.1007/BF00334381