Abstract
We have succeeded in transplanting human osteogenic sarcoma into nude mice. Morphologically, the transplanted tumor is chondrosarcoma and manifests calcification, but not ossification. This tumor is thought to be an excellent model for studying the process of morbid endochondral calcification. In this study, we have used in situ hybridization to examine expression of collagen type I, II, and III mRNAs in this tumor. In situ hybridization was carried out using biotinylated DNA probes. Hybridized probes were detected using a streptavidin-biotin-alkaline phosphatease reagent. The results showed that collagen type I and II mRNAs were produced by cells of the transplanted tumor. Collagen type I mRNA was chiefly localized in the marginal region of the tumor. Collagen type II mRNA, which was predominantly found in the premineralized region of the transplanted tumor, gradually decreased toward the mineralized region. Collagen type III mRNA was not expressed in the transplanted tumor. These results suggest that the character of progenitor chondrogenic cells might be transferred to the transplanted tumor, and that the tumor cells may change the expression of collagen genes with the differentiation or maturation.
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Yasui N, Ono K, Yamaura I, Konomi H, Nagai Y (1983) Immunohistochemical localization of type I, II, and III collagens in the ossified posterior longitudinal ligament of the human cervical spine. Calcif Tissue Int 35:159–163
Stockwell RA (1979) Biology of cartilage cells. Cambridge University Press, Cambridge, England
Haraki T, Oshima O, Kakuta S, Kimura Y, Nagumo M (1992) Localization of types I, II, and X collagen in the transplanted tumor derived from human osteogenic sarcoma. Bone 13:121–128
Oshima O, Leboy PS, McDonald SA, Tuan RS, Shapiro IM (1989) Developmental expression of genes in chick growth cartilage detected by in situ hybridization. Calcif Tissue Int 45:182–192
Tuan RS, Lamb BT, Jesinkey CB (1988) Mouse placental 57-kDa calcium-binding protein: II. Localization of mRNA in mouse and human placentae by in situ cDNA hybridization. Differentiation 37:198–204
McDonald SA, Tuan RS (1988) Expression of collagen type transcripts in chick embryonic bone detected by in situ cDNA-mRNA hybridization. Dev Biol 133:221–234
Sandberg M, Vuorio E (1987) Localization of type I, II, and III collagen mRNA in developing human skeletal tissues by in situ hybridization. J Cell Biol 104:1077–1084
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp 202–203
Brahic M, Haase AT (1978) Detection of viral sequences of low reiteration frequency by in situ hybridization. Proc Natl Acad Sci USA 75:6125–6129
Haase AT, Brahic M, Stowring L, Blum H (1984) Detection of viral nucleic acids by in situ hybridization. Methods Virol 7: 189–226
Singer RH, Lawrence JB, Villnave C (1986) Optimization of in situ hybridization using isotopic and nonisotopic detection method. Bio Techniques 4:230–250
Focht R, Adams SL (1984) Tissue specificity of type I collagen gene expression is determined at both transcriptional and posttranscriptional levels. Mol Cell Biol 4:1843–1852
Bennett VD, Adams SL (1990) Identification of a cartilagespecific promoter within intron 2 of the chick α2(I) collagen gene. J Biochem 265-4:2223–2230
von der Mark K (1980) Immunological studies on collagen type transition in chondrogenesis. Curr Top Dev Biol 14:199–225
Horton WA, Machada MM (1988) Extracellular matrix alterations during endochondral ossification in humans. J Orthop Res 6:793–803
Castagnola P, Dozin B, Moro G, Cancedda R (1988) Changes in the expression of collagen genes show two stages in chondrocytes differentiation in vitro. J Cell Biol 106:461–467
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Oshima, O., Haraki, T., Kakuta, S. et al. Expression of collagen species in a cartilaginous tumor derived from a human osteogenic sarcoma. Calcif Tissue Int 54, 516–520 (1994). https://doi.org/10.1007/BF00334335
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DOI: https://doi.org/10.1007/BF00334335