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Influence of exogenous porcine growth hormone on vitamin D metabolism and calcium and phosphorus absorption in intact pigs

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Abstract

The influence of growth hormone (GH) on vitamin D metabolism and calcium and phosphorus absorption in vivo is not clear. We, therefore, measured calcium and phosphorus balance, plasma 1,25-dihydroxyvitamin D (1,25(OH)2D), and intestinal vitamin D-dependent calcium-binding protein (CaBP 9k) in intact growing pigs given exogenous GH. Six 10-week-old pigs were given two daily subcutaneous injections of 50 μg porcine GH/kg body weight for 2 months; six control pigs were given vehicle. They were all fed a diet containing 1.1% Ca, 0.6% P, and 1000 IU vitamin D3/kg. Apparent Ca and P absorption and retention were measured in a 10-day balance trial at the end of the 2 months. The plasma levels of Ca, P, 1,25(OH)2D, IGF-I, and GH were determined, and the duodenal and jejunal mucosal CaBP 9k content was measured at slaughter. The plasma Ca and P of GH-treated pigs were unchanged, but all aspects of mineral metabolism, including the plasma 1,25(OH)2D concentration (40%), Ca absorption and retention (70%), P absorption (33%) and retention (45%), and jejunal CaBP 9k (40%), were stimulated, in addition to an increase in the circulating IGF-I concentration.

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References

  1. Denis I, Pointillart A, Zerath E, Bonneau M (1992) Effect du traitement par la somatotropine porcine (PST) sur le métabolisme osseux chez le porcelet. J Rech Porcine en France 24:249–254

    Google Scholar 

  2. Goff JP, Caperna TJ, Steele NC (1990) Effects of growth hormone administration on vitamin D metabolism and vitamin D receptors in the pig. Dom Anim Endocrinol 7:425–433

    Google Scholar 

  3. Gray RW, Garthwaite TL (1985) Activation of renal 1,25-dihydroxyvitamin D3 synthesis by phosphate deprivation: evidence for a role for growth hormone. Endocrinology 116:189–193

    Google Scholar 

  4. Halloran BP, Spencer EM (1988) Dietary phosphorus and 1,25-dihydroxyvitamin D metabolism: influence of insulin-like growth factor I. Endocrinology 123:1225–1229

    Google Scholar 

  5. Lund B, Eskildsen PC, Lund B, Norman AW, Sorensen OH (1981) Calcium and vitamin D metabolism in acromegaly. Acta Endocrinol 96:444–450

    Google Scholar 

  6. Spanos E, Barrett D, MacIntyre I, Pike JW, Safilian EF, Haussler MR (1978) Effect of growth hormone on vitamin D metabolism. Nature 273:246–247

    Google Scholar 

  7. Lukaszkiewicz J, Lorenc R, Romer T, Garabedian M, Balsan S, Jones G (1985) Growth hormone induced catch-up growth and serum 1,25(OH)2D concentration in pituitary dwarfs. In: Norman AW, Schaefer K, Grigoleit HG, Von Herrath D (eds) Vitamin D. A chemical, biochemical and clinical update. Walter de Gruyter, Berlin, pp 1109–1110

    Google Scholar 

  8. Harbison MD, Gertner JM (1990) Permissive action of growth hormone on the renal response to dietary phosphorus deprivation. J Clin Endocrinol Metab 70:1035–1040

    Google Scholar 

  9. Aloia JF, Vaswani A, Meunier JP, Edouard CM, Arlot ME Yeh JK, Cohn SH (1987) Coherence treatment of postmenopausal osteoporosis with growth hormone and calcitonin. Calcif Tissue Int 40:253–259

    Google Scholar 

  10. Bouillon R (1991) Growth hormone and bone. Horm Res 36:49–55

    Google Scholar 

  11. Henneman PH, Forbes AP, Moldawer M, Dempsey EF, Carroll EL (1960) Effects of human growth hormone in man. J Clin Invest 39:1223–1238

    Google Scholar 

  12. Beck JC, Mc Garry EE, Dyrenfurth D, Venning EH (1957) Metabolic effects of human and monkey growth hormone in man. Science 125:584–588

    Google Scholar 

  13. Van der Veen EA, Netelenbos JC (1990) Growth hormone (replacement), therapy in adults: bone and calcium metabolism. Horm Res 33 (suppl 4):65–68

    Google Scholar 

  14. Heaney RP, Harris WH, Cockin J Weinberg EH (1972) Growth hormone: the effect on skeletal renewal in the adult dog. II. Mineral kinetic studies. Calcif Tissue Res 10:14–22

    Google Scholar 

  15. Yeh JK, Aloia JF (1984) Effect of hypophysectomy and 1,25-dihydroxyvitamin D on duodenal calcium absorption. Endocrinology 114:1711–1717

    Google Scholar 

  16. Chipman JJ, Zerwekh J, Nicar M, Marks J, Pak CYC (1980) Effect of growth hormone administration: reciprocal changes in serum 1α,25-dihydroxyvitamin D and intestinal calcium absorption. J Clin Endocrinol Metab 51:321–324

    Google Scholar 

  17. Pointillart A, Fontaine N, Thomasset M (1986) Effects of vitamin D on calcium regulation in vitamin D-deficient pigs given a phytate-phosphorus diet. Br J Nutr 56:661–669

    Google Scholar 

  18. Pointillart A, Colin C, Eb M, Thomasset M (1991) Intestinal calbindin-D9K and calcium absorption in the pig. In: Norman AW, Bouillon R, Thomasset M (eds) Vitamin D: gene regulation, structure-function analysis and clinical application. Walter de Gruyter, Berlin, pp 611–612

    Google Scholar 

  19. Bonneau M (1990) Régulation de la croíssance du porc par la somatrotopine et les autres hormones de l'axe somatotrope: sécrétion, mécanismes d'action et effets sur les performances. J Rech Porcine en France 22:51–68

    Google Scholar 

  20. Pointillart A, Fourdin A, Fontaine N (1987) Importance of cereal phytase activity for phytate phosphorus utilization by growing pigs fed diets containing triticale or corn. J Nutr 117:907–913

    Google Scholar 

  21. Pointillart A, Fontaine N, Thomasset M (1984) Phytate phosphorus utilization and intestinal phosphatases in pigs fed low phosphorus: wheat or corn diets. Nutr Rep Int 29:473–482

    Google Scholar 

  22. Pointillart A, Bonneau M, Kann G (1991) Effect of human growth hormone-releasing factor on bone and mineral metabolism in growing pigs. J Anim Sci 69:1454–1460

    Google Scholar 

  23. Stat-ITCF (1988) Manuel d'utilisation. Institut technique des céréales et des fourrages (ed), Paris

  24. Campbell RG, Steele NC, Caperna TJ, McMurtry JP, Solomon MB, Mitchell AD (1988) Interrelationships between energy intake and endogenous procine growth hormone administration on the performance, body composition and protein and energy metabolism of growing pigs weighing 25 to 55 kilograms live weight. J Anim Sci 66:1643–1655

    Google Scholar 

  25. Rudman D, Feller AG, Cohn L, Shetty KR, Rudman IW, Draper MW (1991) Effects of human growth hormone on body composition in elderly Men. Horm Res 36:73–81

    Google Scholar 

  26. Hanna S, Harrison MT, MacIntyre I, Frasier R (1961) Effect of growth hormone on calcium metabolism and magnesium metabolism. Br Med J 2:1215

    Google Scholar 

  27. Pointillart A, Fontaine N, Eb M, Thomasset M (1988) Dietary vitamin D level and mineral (P, Ca) absorption in pigs fed phytic P diets. In: Norman AW, Schaefer K, Grigoleit HG, Herrath DV (eds) Vitamin D: molecular, cellular and clinical endocrinology. Walter de Gruyter, Berlin, pp 1013–1014

    Google Scholar 

  28. Bruns ME, Vollmer SS, Bruns DE, Overpeck JG (1981) Human growth hormone increases the vitamin D-dependent calcium binding protein (CaBP) in the intestine of hypophysectomized rats. (abstract). Calcif Tissue Int 33:331

    Google Scholar 

  29. Spanos E, Brown DJ, Stevenson JC, MacIntyre I (1981) Stimulation of 1,25-dihydroxycholecalciferol production by prolactin and related peptides in intact renal cell preparations in vitro. Biochim Biophys Acta 672:7–12

    Google Scholar 

  30. Condamine I, Friedlander G, Garabedian M (1992) Phosphate deprivation and insulin-like growth factor 1 directly stimulate 1,25(OH)2 vitamin D3 synthesis in cultured kidney cells (abstract) Bone Miner 17 (suppl 1):101

    Google Scholar 

  31. Thomas BR, Spencer EM (1993) 1,25-Dihydroxyvitamin-D3 production in the isolated perfused rat kidney in response to changing perfusate phosphorus concentrations and insulin-like growth factor. J Nutr Biochem 4:158–161

    Google Scholar 

  32. Berndt TJ, Knox FG (1992) Renal regulation of phosphate excretion. In: Seldin DW, Giebisch G (eds) The kidney: physiology and pathophysiology, 2nd ed. Raven Press, New York, pp 2511–2532

    Google Scholar 

  33. Caverzasio J, Bonjour JP (1991) IGF-I, a key regulator of renal phosphate transport and 1,25-dihydroxyvitamin D3 production during growth. NIPS 6:206–210

    Google Scholar 

  34. Lee DBN, Walling MW, Corry DB (1986) Phosphate transport across rat jejunum: influence of sodium, pH, and 1,25-dihydroxyvitamin D3. Am J Physiol 251:G90-G95

    Google Scholar 

  35. Ghishan FK (1992) Phosphate transport by plasma membranes of enterocytes during development: role of 1,25-dihydroxycholecalciferol. Am J Clin Nutr 55:873–877

    Google Scholar 

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Authors and Affiliations

  1. Laboratoire de Nutrition et Sécurité Alimentaire, INRA 78352, Jouy-en-Josas Cédex, France

    I. Denis & A. Pointillart

  2. Hôpital Robert Debré, INSERM U120, 75019, Paris, France

    M. Thomasset

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  1. I. Denis
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  2. M. Thomasset
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  3. A. Pointillart
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Denis, I., Thomasset, M. & Pointillart, A. Influence of exogenous porcine growth hormone on vitamin D metabolism and calcium and phosphorus absorption in intact pigs. Calcif Tissue Int 54, 489–492 (1994). https://doi.org/10.1007/BF00334330

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  • DOI: https://doi.org/10.1007/BF00334330

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