Summary
Kinetic analysis of induction of recA protein synthesis after U.V. irradiation does not show correspondence with the kinetics of division inhibition in lon + and lon - strains, but there is correlation between induction and DNA repair activity. Protein X is stable and identical in both lon + and lon - strains. When the induction of recA protein after U.V. is drastically reduced by rifampicin treatment, no effect on the kinetics of division inhibition is observed.
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Adler, H.I., Hardigree, A.A.: Growth and division of filamentous forms of Escherichia coli. J. Bacteriol. 90, 223–226 (1965)
Blumenthal, R.M., Dennis, P.P.: Gene expression in Escherichia coli during partial Rifampicin-mediated restrictions of transcription initiation. Mol. Gen. Genet. 165, 79–86 (1978)
Boyd, A.: Aspects of outer membrane protein synthesis in Escherichia coli B/r. Ph.D. Thesis, University of Leicester, England (1979)
Castellazzi, M., George, J., Buttin, G.: Prophage induction and cell division in E. coli. I. Further characterisation of a thermosensitive mutantion tif1 whose expression mimics the effect of U.V. irradiation. Mol. Gen. Genet. 119, 139–152 (1972)
Defais, M., Caillet-Fauquet, P., Fox, M.S., Radman, M.: Induction kinetics of mutagenic DNA repair activity in E. coli following irradiation. Mol. Gen. Genet. 148, 125–130 (1976)
Defais, M., Caillet-Fauquet, P., Radman, M., Errera, N.: U.V. reactivation and U.V. mutagenesis of λ in different genetic systems. Virology 43, 495–503 (1971)
Emmerson, P.T., West, S.C.: Identification of protein X of Escherichia coli as the recA +/tif + gene product. Mol. Gen. Genet. 155, 77–85 (1977)
George, J., Castellazzi, M., Buttin, G.: Prophage induction and cell division of E. coli. III. Mutations sfiA and sfiB restore division in tif and lon strains and permit the expression of mutator properties of tif. Mol. Gen. Genet. 140, 309–332 (1975)
Gudas, L.J., Mount, D.W.: Identification of the recA (tif) gene product of Escherichia coli. Proc. Natl. Acad. Sci U.S.A. 74, 5280–5284 (1977)
Gudas, L.J., Pardee, A.B.: A model for the regulation of Escherichia coli DNA repair functions. Proc. Natl. Acad. Sci. U.S.A. 72, 2330–2334 (1975)
Howard-Flanders, P., Simson, E., Theriot, L.: A locus that controls filament formation and sensitivity to radiation in Escherichia coli. Genetics 49, 237–246 (1964)
Inouye, M., Pardee, A.B.: Changes of membrane proteins and their relation to DNA synthesis and cell division of E. coli. J. Biol. Chem. 245, 5813–5819 (1970)
Johnson, B.F.: Fine structure mapping and properties of mutations suppressing the lon mutation in Escherichia coli K12 and B strains. Genet. Res. 30, 273–286 (1977)
Laemmli, U.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685 (1970)
McEntee, K.: Protein X is the product of the recA gene of Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 74, 5275–5279 (1977)
Molnár, J., Holland, I.B., Mándi, Y.: Selection of lon mutants in Escherichia coli by treatment with phenothiazines. Genet. Res. 30, 13–21 (1977)
O'Farrell, P.H.: High resolution two-dimensional electrophoresis of proteins. J. Biol. Chem. 250, 4007–4021 (1975)
Pederson, S., Bloch, P.L., Reeh, S., Neidhardt, F.C.: Patterns of protein synthesis in E. coli: a catalog of the amount of 140 individual proteins at different growth rates. Cell 14, 179–190 (1978)
Radman, M.: In: Molecular mechanisms for repair of DNA. (P.C. Hanawalt, R.B. Setlow, eds.). Basic Life Sci. 5, 355–369 (1975)
Roberts, J.W., Roberts, C.W., Craig, N.L.: Escherichia coli recA gene product inactivates phage λ repressor. Proc. Natl. Acad. Sci. U.S.A. 75, 4714–4718 (1978)
Satta, G., Gudas, L.J., Pardee, A.B.: Degradation of Escherichia coli DNA: Evidence for limitation in vivo by protein X, the recA gene product. Mol. Gen. Genet. 168, 69–80 (1979)
Satta, G., Pardee, A.B.: Inhibition of Escherichia coli division by protein X. J. Bacteriol 133, 1492–1500 (1978)
Smith, K.C.: DNA synthesis in sensitive and resistant mutants of Escherichia coli B after ultraviolet irradiation. Mutat. Res. 8, 481–495 (1969)
Weinstock, G.M., McEntee, K., Lehman, I.R.: ATP dependent renaturation of DNA catalysed by the recA protein of Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 76, 126–130 (1979)
Witkin, E.M.: Ultraviolet mutagenesis and inducible DNA repair in Escherichia coli. Bacteriol. Rev. 40, 869–907 (1976)
Zehnbauer, B., Foley, E.C., Markovitz, A.: DNA binding protein and the lon (capR) gene of E. coli K12. Fed. Proc. 38, 623 (1979)
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Darby, V., Holland, I.B. A kinetic analysis of cell division, and induction and stability of recA protein in U.V. irradiated lon + and lon-strains of Escherichia coli K12. Molec. Gen. Genet. 176, 121–128 (1979). https://doi.org/10.1007/BF00334303
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DOI: https://doi.org/10.1007/BF00334303