Summary
Spontaneous tetracycline-sensitive, transfer-deficient mutants of R100-1 were selected and analysed by genetic complementation tests and with the restriction endonculease EcoR1. While some of the Tets Tra- mutants were caused by a single deletion event which removed the Tetr genes and extended into the neighbouring transfer genes, other mutants were the result of the deletion of the Tetr genes within Tn10 which was accompanied by an inversion of adjacent DNA sequences. A clustering of deletion and inversion endpoints occurred in the traA gene. Some of the transfer genes of R100-1 were assigned to EcoR1 fragments.
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Achtman, M., Kusecek, B., Timmis, K.N.: tra Cistrons and proteins encoded by the Escherichia coli antibiotic resistance plasmid R6-5. Mol. Gen. Genet. 163, 169–179 (1978)
Achtman, M., Willetts, N.S., Clark, A.J.: Beginning a genetic analysis of conjugational transfer determined by the F factor in E. coli by isolation and characterisation of transfer-deficient mutants. J. Bacteriol. 106, 529–538 (1971)
Achtman, M., Willetts, N.S., Clark, A.J.: A conjugational complementation analysis of transfer-deficient mutants of Flac in E. coli. J. Bacteriol. 110, 831–842 (1972)
Cohen, S.N.: Transposable genetic elements and plasmid evolution. Nature (Lond.) 263, 731–738 (1976)
Dempsey, W.B., Willetts, N.S.: Plasmid co-integrates of prophage lambda and R factor R100. J. Bacteriol. 126, 166–176 (1976)
Egawa, R., Hirota, Y.: Inhibition of fertility by multiple drugresistance factor in E. coli K12. Jpn. J. Hum. Genet. 37, 66–69 (1962)
Foster, T.J.: Tetracycline-sensitive mutants of the F-like R factors R100 and R100-1. Mol. Gen. Genet. 137, 85–88 (1975)
Foster, T.J.: R factor-mediated tetracycline resistance in Escherichia coli K12. Dominance of some tetracycline sensitive mutants and relief of dominance by deletion. Mol. Gen. Genet. 143, 339–344 (1976)
Foster, T.J., Howe, T.G.B.: Deletion map of the chloramphenicol resistance region of R1 and R100-1. J. Bacteriol. 116, 1062–1063 (1973)
Foster, T.J., Howe, T.G.B., Richmond, K.M.V.: Translocation of the tetracycline resistance determinant from R100-1 to the Escherichia coli K12 chromosome. J. Bacteriol. 124, 1153–1158 (1975)
Foster, T.J., Walsh, A.: Phenotypic characterisation of R-factor tetracycline resistance determinants. Genet. Res. 24, 333–343 (1974)
Foster, T.J., Willetts, N.S.: Genetic analysis of deletions of R100-1 that are both transfer-deficient and tetracycline sensitive. J. Gen. Microbiol. 93, 133–140 (1976)
Foster, T.J., Willetts, N.S.: Characterisation of transfer-deficient mutants of the R100-1 Tcs plasmid pDU202, caused by insertion of Tn10. Mol. Gen. Genet. 156, 107–114 (1977)
Hu, S., Ohtsubo, E., Davidson, N., Saedler, H.: Electron microscope heteroduplex studies of sequence relations among bacterial plasmids: Identification and mapping of the insertion sequences IS1 and IS2 in F and R plasmids. J. Bacteriol. 122, 764–775 (1975)
Kehoe, M.A., Foster, T.J.,: Genetic analysis of mutations in the transfer genes of pDU202 tra::Tn10 plasmids, caused by the excision of Tn10. Mol. Gen. Genet. 157, 109–118 (1977)
Kleckner, N.: Translocatable elements in procaryotes. Cell 11, 11–23 (1977)
Kleckner, N., Chan, R.K., Tye, B., Botstein, D.: Mutagensis by insertion of a drug-resistance element carrying an inverted repetition. J. Mol. Biol. 97, 561–575 (1975)
Kleckner, N., Reichardt, K., Botstein, D.: Imprecise excision of the translocatable tetracycline-resistance element Tn10: Generation of deletions and inversions (in press 1978)
Miki, T., Horiuchi, T., Willetts, N.S.: Identification and characterization of four new tra cistrons on the E. coli sex factor F. Plasmid 1, 316–323 (1978)
Miller, J.H.: In: Experiments in molecular genetics. New York, Cold Spring Harbour: Cold Spring Harbour Laboratory, 1972
Ohtsubo, E., Nishimura, Y., Hirota, Y.: Transfer-defective mutants of sex factors in Escherichia coli: 1. Defective mutants and complementation analysis. Genetics 64, 173–188 (1970)
Ptashne, K., Cohen, S.N.: Occurrence of insertion sequence (IS) regions on plasmid deoxyribonucleic acid as direct and inverted nucleotide sequence duplications. J. Bacteriol. 122, 776–781 (1975)
Sharp, P.A., Cohen, S.N., Davidson, N.: Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli: II. Structure of drug resistance (R) factors and F factors. J. Mol. Biol. 75, 235–255 (1973)
Shurray, R.A., Nagaishi, H., Clark, A.J.: Molecualr cloning of DNA from the F sex factor of E. coli K12. Proc. Natl. Acad. Sci. U.S.A. 73, 64–68 (1976)
Tanaka, N., Cramer, J.H., Rownd, R.H.: EcoR1 restriction endonuclease map of the composite R plasmid NR1. J. Bacteriol. 127, 619–636 (1976)
Timmis, K.N., Cabello, F., Cohen, S.N.: Cloning and characterisation of EcoR1 and HindIII restriction endonuclease-generated fragments of antibiotic resistance plasmids R6-5 and R6. Mol. Gen. Genet. 162, 121–137 (1978)
Willetts, N.S.: The plasmid-specificity of two proteins required for conjugation in E. coli K12. Nature New Biol. 230, 183–185 (1971)
Willetts, N.S., Achtman, M.: Genetic analysis of transfer by the Escherichia coli sex factor F, using P1 transductional complemention. J. Bacteriol. 110, 843–851 (1972)
Willetts, N.S.: Characterisation of the F transfer cistron, tra L. Genet. Res. 21, 205–213 (1973)
Yoshimoli, A.N.: Genetic and biochemical analysis of the restriction and modification of DNA by resistance transfer factors. Ph. D. thesis, University of California (1971)
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Kehoe, M.A., Foster, T.J. Transposon 10 promoted deletions and inversions in the transfer genes of R100-1. Molec. Gen. Genet. 176, 113–120 (1979). https://doi.org/10.1007/BF00334302
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DOI: https://doi.org/10.1007/BF00334302