Skip to main content
Log in

Temperature-sensitive mutants in cysteinyl-tRNA ligase of E. coli K-12

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

Two mutants with a defective cysteinyl-tRNA synthetase have been found in a collection of spontaneous temperature sensitive mutants. The mutated gene, which is designated cysS, is closely contransduced with purE.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Bachmann, B.J., Low, K.B., Taylor, A.L.: Recalibrated linkage map of Escherichia coli K-12. Bacteriol. Rev. 40, 116–167 (1976)

    Google Scholar 

  • Bohman, K., Isaksson, L.A.: Mutations in the tryptophanyl-transfer ribonucleic acid ligase of E. coli causing temperature-sensitivity for growth. Mol. Gen. Genet. 161, 285–289 (1978)

    Google Scholar 

  • Harris, J.D., Heilig, J.S., Martinez, J.J., Calender, R., Isaksson, L.A.: Temperature sensitive Escherichia coli mutant producing a temperature-sensitive δ subunit of DNA-dependent RNA polymerase. Proc. Natl. Acad. Sci. U.S.A. 75, 6177–6181 (1978)

    Google Scholar 

  • Isaksson, L.A., Sköld, S.E., Skjöldebrand, J., Takata, R.: A procedure for isolation of spontaneous mutants with temperaturesensitive synthesis of RNA and/or protein. Mol. Gen. Genet. 156, 233–237 (1977)

    Google Scholar 

  • Low, K.B.: Escherichia coli K-12 F-prime factors, old and new. Bacteriol. Rev. 36, 587–607 (1972)

    Google Scholar 

  • Miller, J.H.: Experiments in molecular genetics. New York: Cold Spring Harbor Laboratory 1972

    Google Scholar 

  • Wu, T.T.: A model for three-point analysis of random general transduction. Genetics 54, 405–410 (1966)

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Communicated by A. Böck

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bohman, K., Isaksson, L.A. Temperature-sensitive mutants in cysteinyl-tRNA ligase of E. coli K-12. Molec. Gen. Genet. 176, 53–55 (1979). https://doi.org/10.1007/BF00334295

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00334295

Keywords

Navigation