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Nalidixic acid causes a transient G1 arrest in the yeast Saccharomyces cerevisiae

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Summary

The addition of nalidixic acid to growing cells of the yeast Saccharomyces cerevisiae resulted in a transient depression in the rate of ribosomal precursor RNA production and a transient arrest of cells in G1. Protein synthesis rates were less affected. Lower concentrations of nalidixic acid also affected RNA synthesis and progression through G1 but had no effect on protein synthesis rates. We suggest that nalidixic acid has a primary effect on RNA synthesis leading to a G1 arrest.

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References

  • Cashel, M., Lazzarini, R.A., Kalbacher, B.: An improved method for thin-layer chromatography of nucleotide mixtures containing 32P-labelled orthophosphate. J. Chromatogr. 40, 103–109 (1969)

    Google Scholar 

  • Hartwell, L.H.: Saccharomyces cerevisiae cell cycle. Bacteriol. Rev. 38, 164–198 (1974)

    Google Scholar 

  • Hereford, L.M., Hartwell, L.H.: Sequential gene function in the initiation of Saccharomyces cerevisiae DNA synthesis. J. Mol. Biol. 84, 445–461 (1974)

    Google Scholar 

  • Johnston, G.C., Singer, R.A.: RNA synthesis and control of cell division in the yeast Saccharomyces cerevisiae. Cell 14, 951–958 (1978)

    Google Scholar 

  • Johnston, G.C., Singer, R.A., McFarlane, E.S.: Growth and cell division during nitrogen starvation of the yeast Saccharomyces cerevisiae. J. Bacteriol. 132, 723–730 (1977)

    Google Scholar 

  • Michaels, C.A., Blamire, J., Goldfinger, B., Marmur, J.: Studies on the action of nalidixic acid in the yeast Saccharomyces cerevisiae. Antimicrob: Agents Chemother. 3, 562–567 (1973)

    Google Scholar 

  • Smith, C.L., Kubo, M., Imamoto, F.: Promoter-specific inhibition of transcription by antibiotics which act on DNA gyrase. Nature 275, 420–423 (1978)

    Google Scholar 

  • Singer, R.A., Johnston, G.C., Bedard, D.: Methionine analogs and cell division regulation in the yeast Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. U.S.A. 75, 6083–6087 (1978)

    Google Scholar 

  • Wehr, C.T., Kudrna, R.D., Parks, L.W.: Effect of putative deoxyribonucleic acid inhibitors on macromolecular synthesis in Saccharomyces cerevisiae. J. Bacteriol. 102, 636–641 (1970)

    Google Scholar 

  • Whittaker, P.A., Carnevali, R.: Inhibition by nalidixic acid of nucleic acid and protein synthesis in Saccharomyces cerevisiae. Biochem. Soc. Trans. 5, 1503–1505 (1973)

    Google Scholar 

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Authors and Affiliations

  1. Department of Medicine, Biochemistry and Microbiology, Dalhousie University, B3H 4H7, Halifax, Nova Scotia, Canada

    R. A. Singer & G. C. Johnston

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  1. R. A. Singer
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  2. G. C. Johnston
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Communicated by F. Kaudewitz

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Singer, R.A., Johnston, G.C. Nalidixic acid causes a transient G1 arrest in the yeast Saccharomyces cerevisiae . Molec. Gen. Genet. 176, 37–39 (1979). https://doi.org/10.1007/BF00334293

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  • DOI: https://doi.org/10.1007/BF00334293

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