Summary
The plasmid pHA10 provides a good positive selection system for P L promoter mutations. It contains the λEcoR1-D fragment cloned into the pBR322 plasmid. Inactivation of the temperature sensitive λ repressor at 42°C leads to transcription of the kil gene and host death. One group of survivors at 42°C is saved due to mutations in the P L promoter. After several screening procedures one such mutant was isolated. Restriction enzyme analysis of the plasmid DNA followed by sequence determination showed it to be a 24 base pair deletion beginning half-way through the “Pribnow box” continuing through part of the-35 region. It removes the O L1 section of the O L operator plus one base pair of O L2. This mutation (called dpl) may be useful to the study of the λO L P L regulation system. The effect of base changes within the P L promoter on the promoter activity are discussed.
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Communicated by E. Bautz
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Hyman, H.C., Honigman, A. The use of the plasmid pHA10 in the isolation of λP L promoter mutations. Mol Gen Genet 185, 515–517 (1982). https://doi.org/10.1007/BF00334152
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DOI: https://doi.org/10.1007/BF00334152