Summary
An Escherichia coli mutant has been isolated (Hek) in which the kil gene of bacteriophage Mu is not functionally expressed. The hek locus has been mapped between rpoD (66.2 min) and argR (69.5 min) on the E. coli chromosome. No influence of the hek mutation on phage or E. coli development could be detected.
References
Giphart-Gassler M, van de Putte P (1979) Thermo-inducible expression of cloned early genes of bacteriophage Mu. Gene 7: 33–50
Greer H (1975) The kil gene of bacteriophage lambda. Virology 66: 589–604
Low KB (1972) Escherichia coli K-12 F-prime factors, old and new. Bacteriol Rev 36: 587–607
Nakamura Y, Osawa T, Yura T (1977) Chromosomal location of a structural gene for the RNA polymerase σ factor in E. coli. Proc Natl Acad Sci USA 74: 1831–1835
Reiner AM (1969) Isolation and mapping of polynucleotide phosphorylase mutants of E. coli. J Bacteriol 97: 1431–1436
Van de Putte P, Westmaas G, Giphart M, Wijffelman C (1977) On the kil gene of bacteriophage Mu. In: Bukhari AI, Shapiro JA, Adhya S (eds) DNA insertion elements, plasmids and episomes. Cold Spring Harbor Laboratory, New York, pp 287–294
Van de Putte P, Giphart-Gassler M, Goosen T, van Meeteren A, Wijffelman C (1978) Is integration essential for Mu development? In: Hofschneider P, Starlinger P (eds) Integration and excision of DNA molecules. Springer Verlag, Berlin Heidelberg New York, pp 33–40
Van de Putte P, Giphart-Gassler M, Goosen N, Goosen T, van Leerdam E (1981) Regulation of integration and replication functions of bacteriophage Mu. Cold Spring Harbor Symp Quant Biol 45: 347–353
Author information
Authors and Affiliations
Additional information
Communicated by J. Schell
Rights and permissions
About this article
Cite this article
Goosen, N., van de Putte, P. Hek: an Escherichia coli function invoved in functional expression of the kil gene of bacteriophage Mu. Mol Gen Genet 196, 170–172 (1984). https://doi.org/10.1007/BF00334111
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00334111