Summary
About 300 revertants were derived from 44 cob - mutants, mapping in the structure coding regions (exon 1, 3, 4, 5, or 6) of the mitochondrial apocytochrome b gene in Saccharomyces cerevisiae, strain 777-3A. Most of the revertants could not be distinguished from the wild-type by means of physiological properties. Twenty-two revertants different in phenotype are described here in more detail.
The suppressor mutations (sup a) that compensate the primary cob - mutations (i.e., restore growth on glycerol) are mitochondrially inherited. They were localized in the same cob exon regions as the respective primary mutations, except for one revertant with a primary mutation in exon 6 and a suppressor, 4.2 map units distant, which may be located either in intron 5 or downstream in exon 6.
Of 21 suppressors 17 are closely coupled to the primary mutation with recombination frequencies of ≦0.1%–0.3%. An estimate predicts that in more than 80% of these revertants only one amino acid is altered at that point of the polypeptide corresponding to the cob - site in the gene.
The most interesting revertant phenotypes are: (1) reduced growth rate on glycerol. The respective cob -/supa mutations are scattered over the whole cob region and cannot be correlated exclusively with special gene regions. (2) decreased cytochrome b content. The most extreme reductions (28% and 30% of wild-type level) were observed to be due to mutations located in the 5′ proximal part of exon 1. The highest percentage of revertants with decreased cytochrome b content was predominantly found mapping in exon 3. Complications in protoporphyrin attachment or the chelatase reaction were assumed to be the basic lesion causing reduced cytochrome b content, since in 10 out of 11 revertants examined the polypeptide is produced at wild-type level. (3) shifted maximum absorption wavelength of cytochrome b. The double mutations of the respective revertants map in the middle part of exon 1, in exon 4 and exom 5. The corresponding regions in the polypeptide presumably surround the heme group.
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Communicated by W. Gajewski
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Burger, G. Cytochrome b of cob revertants in yeast. Mol Gen Genet 196, 158–166 (1984). https://doi.org/10.1007/BF00334109
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DOI: https://doi.org/10.1007/BF00334109