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Cotransfer and phenotypic stabilisation of syntenic and asyntenic mink genes into mouse cells by chromosome-mediated gene transfer

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Summary

By means of metaphase chromosomes, the genes for mink thymidine kinase (TK) and hypoxanthine-phosphoribosyltransferase (HPRT) were transferred to mutant mouse cells, LMTK-, A9 (HPRT-) and teratocarcinoma cells, PCC4-aza 1 (HPRT-). Eighteen colonies were isolated from LMTK- (series A), 9 from A9 (series B) and none from PCC4-aza 1. The transformed clones contained mink TK or HPRT. Analysis of syntenic markers in series B demonstrated that one clone contained mink glucose-6-phosphate dehydrogenase (G6PD) and the other alpha-galactosidase; in series A, nine clones contained mink galactokinase (GALK) and six mink aldolase C (ALDC). Analysis of 12 asyntenic markers located in ten mink chromosomes showed the presence of only aconitase-1 (ACON1) (the marker of mink chromosome 12) in three clones of series A. The clones lost mink ACON1 between the fifth to tenth passages. Cytogenetic analysis established the presence of a fragment of mink chromosome 8 in eight clones of series A, but not in series B. The clones of series A lost mink TK together with mink GALK and ALDC during back-selection; in B, back-selection retained mink G6PD. No stable TK+ phenotype was detected in clones with a visible fragment of mink chromosome 8. Stability analysis demonstrated that about half of the clones of series B have stable HPRT+ phenotype whereas only three clones of series A have stable TK+ phenotype. It is suggested that the recipient cells, LMTK- and A9, differ in their competence for genetic transformation and integration of foreign genes.

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Authors and Affiliations

  1. Institute of Cytology and Genetics, Siberian Branch of Academy of Sciences of the USSR, Novosibirsk-90, USSR

    M. A. Sukoyan, N. M. Matveeva, S. D. Pack, A. A. Gradov, A. G. Shilov, N. S. Zhdanova & O. L. Serov

  2. Institute of Organic Chemistry, Siberian Branch of Academy of Sciences of the USSR, Novosibirsk-90, USSR

    N. D. Belyaev

Authors
  1. M. A. Sukoyan
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  2. N. M. Matveeva
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  3. N. D. Belyaev
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  4. S. D. Pack
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  5. A. A. Gradov
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  6. A. G. Shilov
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  7. N. S. Zhdanova
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  8. O. L. Serov
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Communicated by H. Böhme

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Sukoyan, M.A., Matveeva, N.M., Belyaev, N.D. et al. Cotransfer and phenotypic stabilisation of syntenic and asyntenic mink genes into mouse cells by chromosome-mediated gene transfer. Mol Gen Genet 196, 97–104 (1984). https://doi.org/10.1007/BF00334099

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  • DOI: https://doi.org/10.1007/BF00334099

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