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Chromosomal location of the Escherichia coli cytochrome b556 gene, cybA

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Summary

The amounts of cytochrome b556 in the cytoplasmic membranes of several Escherichia coli K12 strains having F-prime factors and a lambda transducing phage were determined. The amount was amplified about two-fold in strains having F100-12 and F152, but not in strains having F100-11, F8 and λpsu + 2glnS +. The strain TK3D11, which lacks the kdp-gltA region (deletion D-01) of the E. coli chromosome, did not synthesize cytochrome b556 at all. From these results, the gene cybA encoding cytochrome b556 was located in the kdp-gltA region.

In the cytochrome b556-deficient mutant, a novel b type cytochrome, cytochrome b561 which is a product of the gene cybB, was identified. It seems to function as a physiological electron transferring cytochrome in place of cytochrome b556 in this mutant.

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Abbreviations

HPLC:

high performance liquid chromatography

EDTA:

ethylenediamine tetraacetic acid

SDS:

sodium dodecyl sulfate

NADH:

reduced form of nicotinamide adenine dinucleotide

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Authors and Affiliations

  1. Department of Biology, Faculty of Science, University of Tokyo, 113, Hongo, Tokyo, Japan

    Hiroshi Murakami & Yasuhiro Anraku

  2. Department of Parasitology, Juntendo University, School of Medicine, 113, Hongo, Tokyo, Japan

    Kiyoshi Kita & Hiroshi Oya

Authors
  1. Hiroshi Murakami
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  2. Kiyoshi Kita
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  3. Hiroshi Oya
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  4. Yasuhiro Anraku
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Communicated by M. Takanami

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Murakami, H., Kita, K., Oya, H. et al. Chromosomal location of the Escherichia coli cytochrome b556 gene, cybA . Mol Gen Genet 196, 1–5 (1984). https://doi.org/10.1007/BF00334084

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  • DOI: https://doi.org/10.1007/BF00334084

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